中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6985-6988.doi: 10.3969/j.issn.1673-8225.2011.37.034

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

重组人促红细胞生成素后处理缺血/再灌注前后骨骼肌诱导型一氧化氮合酶及一氧化氮的变化

张  谦1，耿喜林1，杨同群1，冯宝明2，李惠萍3，史继德1，王秉义1   

  1. 1兰州大学第二医院急救中心创伤外科，甘肃省兰州市  730030
    2甘肃省第三人民医院药剂科，甘肃省兰州市 730020
    3甘肃省紧急医疗救援中心医教科，甘肃省兰州市  730030
  • 收稿日期:2011-06-05 修回日期:2011-08-07 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 张谦，男，1967年生，陕西省人，汉族，1991年于兰州医学院毕业，副主任医师，主要从事急诊创伤骨科疾病研究。 zqzyz@126.com
  • 作者简介:张谦，男，1967年生，陕西省人，汉族，1991年于兰州医学院毕业，副主任医师，主要从事急诊创伤骨科疾病研究。 zqzyz@126.com

Effects of recombinant human erythropoietin postconditioning on inducible nitric oxide synthase and nitric oxide in rabbit hindlimb gastrocnemius before and after ischemia/reperfusion injury

Zhang Qian1, Geng Xi-lin1, Yang Tong-qun1, Feng Bao-ming2, Li Hui-ping3, Shi ji-de1, Wang Bing-yi1   

  1. 1Department of Emergency Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China
    2Department of Pharmacy, Third People’s Hospital of Gansu Province, Lanzhou 730020, Gansu Province, China
    3Department of Medical Education, Emergency Medical Rescue Center in Gansu Province, Lanzhou  730030, Gansu Province, China
  • Received:2011-06-05 Revised:2011-08-07 Online:2011-09-10 Published:2011-09-10
  • Contact: Zhang Qian, Department of Emergency Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China zqzyz@126.com
  • About author:Zhang Qian, Associate chief physician, Department of Emergency Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China zqzyz@126.com

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被引次数

3

摘要：

背景：促红细胞生成素对包括心脑在内的多种组织器官的缺血/再灌注损伤有保护作用。
目的：观察重组人促红细胞生成素后处理对家兔后肢腓肠肌缺血/再灌注前后诱导型一氧化氮合酶、一氧化氮和超微结构的影响。
方法：将家兔随机分为空白对照组、模型组和重组人促红细胞生成素组，后2组建立兔左后肢腓肠肌缺血/再灌注实验模型，其中重组人促红细胞生成素组在兔左后肢缺血2 h后静脉注射重组人促红细胞生成素。
结果与结论：再灌注后4，12 h，重组人促红细胞生成素组诱导型一氧化氮合酶表达水平及一氧化氮浓度增高幅度较模型组低(P < 0.05)。电镜下腓肠肌细胞超微结构显示，模型组内皮细胞膜溶解，肿胀明显，肌纤维内线粒体水肿。重组人促红细胞生成素组肌纤维损伤较轻，肌节内Z线及肌节内各带结构基本正常，大部分线粒体结构正常，显示重组人促红细胞生成素组超微结构损伤程度明显轻于模型组。结果证实，重组人促红细胞生成素后处理可通过抑制诱导型一氧化氮合酶蛋白的表达，减少再灌注后过量一氧化氮生成，改善骨骼肌缺血/再灌注损伤。

关键词: 重组人促红细胞生成素, 骨骼肌, 缺血/再灌注损伤, 诱导型一氧化氮合酶, 一氧化氮, 超微结构, 组织构建

Abstract:

BACKGROUND: In recent years, many studies have shown that erythropoietin plays a protective role in many tissues and organs from ischemia/reperfusion injury including the heart and brain.
OBJECTIVE: To observe the effects of recombinant human erythropoietin postconditioning on inducible nitric oxide synthase, nitric oxide and ultrastructure in rabbit hindlimb gastrocnemius before and after ischemia/reperfusion injury.
METHODS: Adult rabbits were randomly divided into three groups: blank control, model, and recombinant human erythropoietin postconditioning. The rabbits in the latter two groups were created into experimental models of ischemia/reperfusion injury of left hind limb gastrocnemius. At 2 hours after ischemia, the rabbits in the recombinant human erythropoietin postconditioning group were intravenously injected with recombinant human erythropoietin.
RESULTS AND CONCLUSION: At 4 and 12 hours after reperfusion, inducible nitric oxide synthase and nitric oxide levels were significantly lower in the recombinant human erythropoietin postconditioning group than in the model group (P < 0.05). The electronic microscope results of gastrocnemius cell ultrastructure showed that in the model group, endothelial cell membrane was lysed and obviously swollen, and mitochondrion in the muscle fiber was also swollen; compared with the model group, the muscle fiber injury was obviously mild in the recombinant human erythropoietin postconditioning group: Z line and the structure of each band within the sarcomere was basically normal, and the structure of most mitochondria was normal. These results showed that recombinant human erythropoietin postconditioning can downregulate the expression of inducible nitric oxide synthase protein expression and decrease nitric oxide levels after perfusion, and thereby improve ischemia/reperfusion injury of the skeletal muscle.

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