中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6737-6740.doi: 10.3969/j.issn.1673-8225.2011.36.020

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

干细胞生长因子和粒细胞集落刺激因子与心肌梗死大鼠体内骨髓间充质干细胞的迁移

吴祖常1，2，吴国才1，吴智明1，熊  丹1，吕俊廷1，杨志刚1   

  1. 1广东医学院附属医院血液内科/广东医学院检验学院临床血液检验学教研室，广东省湛江市 524001
    2佛山市第一人民医院临床医学研究所，广东省佛山市  528000
  • 收稿日期:2011-03-04 修回日期:2011-04-20 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 杨志刚，博士，教授，广东医学院附属医院血液内科，广东医学院检验学院临床血液检验学教研室，广东省湛江市524001 yangzhigangcn@yahoo.com.cn
  • 作者简介:吴祖常★，男，1983年生，广东省英德市人，2010年广东医学院毕业，硕士，主要从事实验血液学方面研究。 wuzuchang@163.com
  • 基金资助:

    广东省科技计划资助项目(2008B03030 1021)。

Stem cell factor and granulocyte colony-stimulating factor affect the migration of bone marrow mesenchymal stem cells in rats with myocardial infarction

Wu Zu-chang1,2, Wu Guo-cai1, Wu Zhi-ming1, Xiong Dan1, Lü Jun-ting1, Yang Zhi-gang1   

  1. 1Department of Hematology, Affiliated Hospital of Guangdong Medical College, Zhanjiang  524001, Guangdong Province, China
    2Institute of Clinical Medicine, the First People’s Hospital of Foshan, Foshan  528000, Guangdong Province, China
  • Received:2011-03-04 Revised:2011-04-20 Online:2011-09-03 Published:2011-09-03
  • Contact: Yang Zhi-gang, M.D., Professor, Department of Hematology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China yangzhigangcn@yahoo.com.cn
  • About author:Wu Zu-chang★, Master, Department of Hematology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China; Institute of Clinical Medicine, the First People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China wuzuchang@163.com
  • Supported by:

    the Science and Technology Project of Guangdong Province, No. 2008B030301021*

PDF

403

被引次数

12

摘要：

背景：外周静脉移植间充质干细胞只有1%~5%的移植细胞能归巢到心肌梗死区域。
目的：观察干细胞生长因子、粒细胞集落刺激因子对骨髓间充质干细胞归巢的影响。
方法：采用贴壁培养法分离培养SD大鼠骨髓间充质干细胞，取传至3~5代细胞。建立SD大鼠急性心肌梗死模型，干细胞生长因子组、粒细胞集落刺激因子组、干细胞生长因子+粒细胞集落刺激因子组在骨髓间充质干细胞移植前3 d和移植后3 d单独或混合皮下注射干细胞生长因子、粒细胞集落刺激因子，骨髓间充质干细胞组不注射细胞因子。
结果与结论：荧光显微镜下观察，骨髓间充质干细胞迁移至心肌梗死组织，骨髓间充质干细胞组、干细胞生长因子组、粒细胞集落刺激因子组迁移至心肌梗死区的骨髓间充质干细胞数量没有明显的区别(P > 0.05)，干细胞生长因子+粒细胞集落刺激因子组的骨髓间充质干细胞数量明显高于其他3组(P < 0.05)。免疫荧光组织化学显示，植入的部分骨髓间充质干细胞表达心肌特异蛋白cTnI。结果说明干细胞生长因子和粒细胞集落刺激因子两种细胞因子联合应用可以促进骨髓间充质干细胞归巢至心肌梗死区域，在体内微环境的诱导下，骨髓间充质干细胞能够转化为心肌样细胞。

关键词: 干细胞生长因子, 骨髓间充质干细胞, 粒细胞集落刺激因子, 心肌梗死, 诱导分化, 归巢

Abstract:

BACKGROUND: Only 1%-5% of the transplanted mesenchymal stem cells (MSCs) home to the myocardial infarction area through peripheral venous transplantation.
OBJECTIVE: To investigate the effects of stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) on MSCs homing.
METHODS: MSCs were isolated from SD rats, and purified by adhesive-screening method. MSCs at passages 3-5 were assigned to 4 groups: groups of MSCs, SCF, G-CSF and SCF+G-CSF.
RESULTS AND CONCLUSION: Under fluorescence microscope, the number of MSCs migrating to the myocardial infarction region had significant difference among MSCs group, SCF group and G-CSF group (P > 0.05), but SCF+G-CSF group had the higher number of MSCs migrating to the myocardial infarction region than other three groups (P < 0.05). Immunofluorescence histochemistry showed that some MSCs express cardiac-specific protein cTnI. Combination of SCF and G-CSF can promote MSCs homing, and induced by microenvironment in vivo, MSCs can convert into cardiomyocyte-like cells.

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