中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (35): 6567-6570.doi: 10.3969/j.issn.1673-8225.2011.35.025

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

静磁场作用下金属离子对成骨细胞的毒性

周通华1，戴  闽1，程细高1，詹  平1，熊  皞1，邹文楠2，严小青2   

  1. 1南昌大学第一附属医院骨二科，江西省南昌市  330006
    2南昌大学建筑工程学院工程力学实验中心，江西省南昌市  330046
  • 收稿日期:2011-03-29 修回日期:2011-06-18 出版日期:2011-08-27 发布日期:2011-08-27
  • 通讯作者: 戴闽，南昌大学第一附属医院骨二科，江西省南昌市330006
  • 作者简介:周通华★，男，1984年生，江西省南昌市人，汉族，2011年南昌大学毕业，硕士，医师，主要从事人工关节的研究。 butong2006@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30760255)；南昌大学学科交叉基金(Z03722)。

Static magnetic field reduces the toxic effect of metal ions on osteoblasts

Zhou Tong-hua1, Dai Min1, Cheng Xi-gao1, Zhan Ping1, Xiong Hao1, Zhou Wen-nan2, Yan Xiao-qing2   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
    2Experimental Center of Engineering Mechanics, Engineering College of Nanchang University, Nanchang  330046, Jiangxi Province, China
  • Received:2011-03-29 Revised:2011-06-18 Online:2011-08-27 Published:2011-08-27
  • Contact: Dai Min, Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Zhou Tong-hua★, Master, Physician, Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China butong2006@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30760255*; Interdisciplinary Fund of Nanchang University, No. Z03722*

PDF

353

被引次数

2

摘要：

背景：体内体外实验研究证明，静磁场具有促进成骨细胞分化及骨形成的作用。
目的：观察静磁场作用下金属离子对成骨细胞毒性的影响。
方法：将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液，将金属离子Co2+、Cr3+分别与小鼠颅骨成骨细胞(MC3T3-E1)在不同的磁场强度(1，10，100 mT)下共培养。实验分4组：设Co2++Cr3+组(对照组)和Co2++Cr3+组分别+1，10，100 mT组。
结果与结论：恒磁场作用下的成骨细胞呈现更加成熟的形态学特征，Co2+、Cr3+金属离子对成骨细胞MC3T3的毒性明显降低；同时G2M(分裂期)分布比例明显增加，细胞停滞在G0G1(休眠期)的比率明显减少；与对照组相比，不同场强磁场加载后，成骨细胞碱性磷酸酶活性明显增强(P < 0.05)。提示一定强度的静磁场可拮抗Co2+、Cr3+金属离子对成骨细胞的毒性作用。

关键词: 静磁场, 成骨细胞, 金属离子, 无菌性松动, 体外实验

Abstract:

BACKGROUND: Static magnetic field can promote the proliferation, differentiation and functional activity of osteoblasts.
OBJECTIVE: To investigate the toxic effect of metal ions Co2+, Cr3+ on osteoblasts under the static magnetic field.
METHODS: CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mouse calvarial osteoblasts (MC3T3-E1) were cultured with Co2+ and Cr3+ ions in different magnetic field of 1, 10, 100 mT. Cells only cultured with Co2+ and Cr3+ ions were taken as control group.
RESULTS AND CONCLUSION: Under the static magnetic field, osteoblasts showed more mature morphological features; the toxic effect of metal ions Co2+ and Cr3+ on osteoblasts reduced significantly; G2M (dividing phase) distribution ratio increased and G0G1 (dormancy stage) distribution ratio decreased; the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously. The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2+ and Cr3+ on osteoblasts.

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