中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (34): 6276-6280.doi: 10.3969/j.issn.1673-8225.2011.34.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

重组人骨形态发生蛋白2-肝素-人工骨复合材料的骨诱导活性

周智优1，何水连1，孙  莉1，王丁丁2，汪  炬1   

  1. 1暨南大学生命科学技术学院生物工程研究所，广东省广州市  510632
    2广东药学院生命科学与生物制药学院，广东省广州市  510006
  • 收稿日期:2011-05-24 修回日期:2011-07-07 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 汪炬，博士，副教授，暨南大学生命科学技术学院生物工程研究所，广东省广州市 510632 wang_ju1688@yahoo.cn
  • 作者简介:周智优★，男，1986年生，湖南省长沙市人，暨南大学在读硕士，主要从事生物材料及基因药物研究。 gikyo@yahoo.cn
  • 基金资助:

    省科技攻关项目(2009B030801237)；广东省教育部科技部企业科技特派员行动计划项目(2009B090600134)。

Bioactivities of recombinant human bone morphogenetic protein 2-heparin artificial bone composite materials

Zhou Zhi-you1, He Shui-lian1, Sun Li1, Wang Ding-ding2, Wang Ju1   

  1. 1Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou  510632, Guangdong Province, China
    2College of Life Science and Biological Pharmacy, Guangdong Pharmaceutical University, Guangzhou  510006, Guangdong Province, China
  • Received:2011-05-24 Revised:2011-07-07 Online:2011-08-20 Published:2011-08-20
  • Contact: Wang Ju, Doctor, Associate professor, Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China wang_ju1688@yahoo.cn
  • About author:Zhou Zhi-you★, Studying for master’s degree, Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China gikyo@yahoo.cn
  • Supported by:

    Guangdong Provinical Science and Technology Tackle Key Program, No. 2009B030801237*; the Enterprise Technological Special Correspondent Action Plan by Guangdong Province & Ministry of Education &Ministry of Science, No. 2009B090600134*

PDF

294

被引次数

3

摘要：

背景：已有将重组人骨形态发生蛋白2应用于骨再生及修复的报道，但由于其在生物体内半衰期短而导致诱导骨形成的能力受到限制。
目的：制备具有较好缓释效果的重组人骨形态发生蛋白2-肝素-人工骨复合材料，并检测其缓释性能及骨诱导活性。
方法：通过高效液相色谱法检测重组人骨形态发生蛋白2-肝素复合物对于酶解的保护作用。将重组人骨形态发生蛋白2与肝素溶液混匀后复合于人工骨材料表面，ELISA方法检测其体外释药性质，茜素红染色法检测其诱导成骨细胞的能力，应用小鼠体内实验评价其异位骨诱导能力。
结果与结论：成功制备了具有良好缓释效果的重组人骨形态发生蛋白2-肝素-人工骨复合材料，具有较强的诱导骨钙蛋白及异位骨形成能力。

关键词: 重组人骨形态发生蛋白2, 肝素, 人工骨复合材料, 缓释, 骨形成, 异位骨

Abstract:

BACKGROUND: Recombinant human bone morphogenetic protein 2 (rhBMP-2) has been applied to bone regeneration and repairing, but its ability to induce bone formation is limited because of its short half-life in vivo.
OBJECTIVE: To prepare rhBMP-2-heparin composite materials and to evaluate rhBMP-2 sustained delivery and osteoinductive effects.
METHODS: The effect of heparin on protecting rhBMP-2 from enzyme digestion was assayed by high-performance liquid chromatography. The materials were combined with rhBMP-2-heparin to produce rhBMP-2-heparin artificial bone composite materials, detecting the rhBMP-2 release effect by ELISA. Alizarin red staining was used to detect osteogenic ability. The ability to induce ectopic bone was detected through an in vivo mouse experiment.
RESULTS AND CONCLUSION: It is successful to prepare rhBMP-2 artificial composite materials with good sustained release effect of rhBMP-2, which can effectively induce osteocalcin and ectopic bone formation.

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