中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5975-5978.doi: 10.3969/j.issn.1673-8225.2011.32.019

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

胎儿真皮间充质干细胞中的全能性相关转录因子的表达

杨少光，邢  文，田  堃，刘  蒙，卢士红，赵钦军，任红英，庞爱明，池  颖，马凤霞，韩忠朝   

  1. 中国医学科学院北京协和医学院血液学研究所血液病医院，实验血液学国家重点实验室，天津市300020
  • 收稿日期:2011-02-01 修回日期:2011-05-13 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 韩忠朝，博士，教授，中国医学科学院北京协和医学院血液学研究所血液病医院，实验血液学国家重点实验室，天津市300020 tihzchan@public.tpt.tj.cn
  • 作者简介:杨少光，男，1953年生，重庆市人，汉族，1977年中山医科大学毕业，副主任技师，主要从事血液病相关造血干细胞和再造血微环境的研究。 Yangsg666@163.com 并列第一作者：邢文★，男，1969年生，山东省莱阳市人，汉族，2004年上海第二医科大学毕业，硕士，助理研究员，主要从事实验血液学方面的研究。 xw2318@yahoo.com.cn
  • 基金资助:

    国家973项目(2011CB964800)，名称：造血干细胞维持、衰老与再生的调控机制研究。国家自然科学基金(30900557)，名称：间充质干细胞对正常造血干细胞和白血病干细胞的作用。天津市应用基础与前沿技术研究计划项目(09JCYBJC11000)，名称：抗内皮细胞噬菌体抗体在HSCT内皮损伤研究中的作用。

Detection of pluripotent transcription factors in fetal dermis-derived mesenchymal stem cells

Yang Shao-guang, Xing Wen, Tian Kun, Liu Meng, Lu Shi-hong, Zhao Qin-jun, Ren Hong-ying, Pang Ai-ming, Chi Ying, Ma Feng-xia, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300020, China
  • Received:2011-02-01 Revised:2011-05-13 Online:2011-08-06 Published:2011-08-06
  • Contact: Han Zhong-chao, Doctor, Professor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China tihzchan@public.tpt.tj.cn
  • About author:Yan Shao-guang, Associate chief technician, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China Yangsg666@163.com Xing Wen, Master, Assistant researcher, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China xw2318@yahoo.com.cn Yang Shao-guang and Xing Wen contributed equally to this paper.
  • Supported by:

    the National 973 Program of China, No. 2011CB964800*; the National Natural Science Foundation of China, No. 30900557*; the Applied Basic and Cutting-edge Technology Research Plan of Tianjin City, No.09JCYBJC11000*

PDF

375

被引次数

4

摘要：

背景：人真皮纤维母细胞或间充质干细胞可形成诱导性多能干细胞，但不同研究者所用的转录因子组合却并不相同。
目的：分离人胎儿真皮间充质干细胞，检测其全能性相关转录因子的表达。
方法：水囊引产5月龄胎儿，按照既往分离培养间充质干细胞的方法，得到胎儿真皮间充质干细胞。
结果与结论：胎儿真皮间充质干细胞高表达Oct4和C-myc、中度表达Sox2，是形成诱导性多能干细胞较好的体细胞。

关键词: 胎儿真皮, 间充质干细胞, 诱导性多能干细胞, 全能性, 转录因子

Abstract:

BACKGROUND: The induced pluripotent stem (iPS) cells have been reprogrammed from human dermal fibroblasts and mesenchymal stem cells (MSCs). Various combinations of transcription factors are used by different investigators.
OBJECTIVE: To detect the pluripotent transcription factors expressed in fetal dermis-derived MSCs. 
METHODS: Using previous methodology in isolation and cultivation of MSCs, fetal dermis-derived MSCs were obtained from 5-month-old fetus, inducing abortion by water bag.
RESULTS AND CONCLUSION: Transcription factors of Oct4 and C-myc were expressed highly and Sox2 was expressed moderately. These results suggest that fetal dermis-derived MSCs are better somatic cells for iPS cells.

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