中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (25): 4597-4601.doi: 10.3969/j.issn.1673-8225.2011.25.010

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

细菌纤维素减轻兔耳增生性瘢痕的作用

邱  竣1，柳大烈1，张  阳2，王晋煌1，奚廷斐3，张志雄3，赖  琛3，钟春燕4，盛高铭1   

  1. 1南方医科大学珠江医院整形美容外科，广东省广州市  510282
    2 解放军第四五八医院整形外科，广东省广州市  510602
    3北京大学深圳研究院，生物医学工程研究中心，广东省深圳市 518057
    4海南椰国食品有限公司，海南省海口市 570311
  • 收稿日期:2010-11-20 修回日期:2010-12-20 出版日期:2011-06-18 发布日期:2014-01-10
  • 通讯作者: 柳大烈，主任医师，教授，博士生导师，南方医科大学珠江医院整形美容科主任，广东省广州市 510282
  • 作者简介:邱竣★，男，1984年生，江西省上饶市人，汉族，南方医科大学珠江医院在读硕士，医师，主要从事整形外科的研究。 303548558@qq.com

Effects of bacterial cellulose on reducing hypertrophic scar in rabbit ears

Qiu Jun1, Liu Da-lie1, Zhang Yang2, Wang Jin-huang1, Xi Ting-fei3, Zhang Zhi-xiong3, Lai Chen3, Zhong Chun-yan4, Sheng Gao-ming1   

  1. 1Department of Plastic Surgery, Zhujiang Hospital, South Medical University, Guangzhou  510282, Guangdong Province, China
    2Department of Plastic Surgery, the 458 Hospital of Chinese PLA, Guangzhou  510602, Guangdong Province, China
    3Biomedical Engineering Research Center, Peking University Shenzhen Graduate School, Shenzhen  518057, Guangdong Province, China
    4Hainan Yeguo Foods Co., Ltd., Haikou  570311, Hainan Province, China
  • Received:2010-11-20 Revised:2010-12-20 Online:2011-06-18 Published:2014-01-10
  • Contact: Liu Da-lie, Chief physician, Professor, Doctoral supervisor, Department of Plastic Surgery, Zhujiang Hospital, South Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Qiu Jun★, Studying for master’s degree, Physician, Department of Plastic Surgery, Zhujiang Hospital, South Medical University, Guangzhou 510282, Guangdong Province, China 303548558@qq.com

PDF

360

被引次数

10

摘要：

背景：国内外已有研究报道细菌纤维素对皮肤创伤愈合具有促进作用，但是其对增生性瘢痕是否有治疗作用尚不清楚。
目的：观察细菌纤维素对兔耳增生性瘢痕的疗效。
方法：建立兔耳腹侧增生性瘢痕模型，术后第21天创面上皮化后，对每只兔耳5个不同瘢痕面随机给予5种不同处理方式：持水性分别为1∶5，1∶6，1∶8细菌纤维素组、阳性对照组(贴敷瘢痕贴)、阴性对照组(未贴任何敷料且瘢痕自然生长)。观察不同处理后第0，14，21，28，42，56天瘢痕面大体形态学及组织学变化。
结果与结论：持水性1∶5，1∶6，1∶8细菌纤维素组瘢痕增生厚度低于阴性对照组，但高于阳性对照组(P < 0.01)。与阴性对照组比较，持水性1∶5，1∶6，1∶8细菌纤维素组瘢痕组织中真皮层薄，成纤维细胞少，胶原纤维较细、排列较整齐；与阳性对照组组比较，持水性1∶5，1∶6，1∶8细菌纤维素组成纤维细胞数稍多，胶原也稍粗、排列也稍不整齐。3种细菌纤维素组间瘢痕厚度及成纤维细胞数量为1∶5细菌纤维素组> 1∶6细菌纤维素组> 1∶8细菌纤维素组(P  < 0.05)。说明细菌纤维素有效抑制了兔耳创面愈合后增生性瘢痕的形成，并且持水性越高，效果越好。

关键词: 细菌纤维素, 纳米生物材料, 动物模型, 增生性瘢痕, 胶原

Abstract:

BACKGROUND: Bacterial cellulose (BC) as a kind of new biological materials for the wound healing before scarring has been reported in the world, but the efficacy of bacterial cellulose used in hypertrophic scar after wound healing remains unclear.
OBJECTIVE: To investigate the effects of BC on reducing rabbit ear hypertrophic scar.
METHODS: Hypertrophic scar model were established in rabbit ears. At 21 days postoperative wound epithelization was observed, five different scars of each rabbit ear which were given different treatments were randomly divided into five groups: patching absorbent film (water holding capacity: 1:5, 1:6, 1:8) as the BC treated groups, patching silicone scar film as a positive control group, without sticking any litter and natural growth of scar as a negative control group. At 0, 14, 21, 28, 42, 56 days of givening scar surface different treatments, the development process of hypertrophic scar  was observed grossly and histologically
RESULTS AND CONCLUSION: The scars tissue hyperplasia thickness of the BC treated groups was lower than that of negative control group, but higher than that of positive control group (P < 0.01). Compared with the negative control group, the dermal thickness in scars was thinner, number of fibroblasts was fewer, collagen fibers in scars were losser and more regular in the BC treated groups; compared with the positive control group, number of fibroblasts was slightly increased, collagen fibers in scars were slightly denser and irregular in the BC treated groups. The number of fibroblasts and the scars tissue thickness in the BC treated-group were compared, the BC (1: 5) treated-group > the BC (1: 6) treated-group > the BC (1: 8) treated-group (P< 0.05). It has been proved that BC effectively inhibits the rabbit ear hyperplastic scar after the wound healing. and the more water content the BC has, the better effect it appears in hypertrophic scar.

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