中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (8): 1149-1155.doi: 10.12307/2022.1014

• 组织构建实验造模 experimental modeling in tissue construction •    下一篇

运动强度与高脂饮食模型大鼠脂代谢紊乱和氧化应激的相关性

阮  凌，王光华，吴荣平，晋  战，吕镇庆，张  楠，李寿邦   

  1. 西安石油大学体育系，陕西省西安市  710065
  • 收稿日期:2021-12-13 接受日期:2022-02-15 出版日期:2023-03-18 发布日期:2022-07-27
  • 通讯作者: 李寿邦，博士，教授，硕士生导师，西安石油大学体育系，陕西省西安市 710065
  • 作者简介:阮凌，女 ，1983年生，陕西省安康市人，汉族，2013年华南师范大学毕业，博士，副教授，主要从事运动与慢性病的研究。
  • 基金资助:
    陕西省自然科学基础研究计划项目(2021JQ-586)，项目名称：运动通过调控细胞自噬实现对NAFLD的保护及其机制研究，项目负责人：阮凌

Correlation between exercise intensity and lipid metabolism disorder and oxidative stress in a high-diet rat model

Ruan Ling, Wang Guanghua, Wu Rongping, Jin Zhan, Lyu Zhenqing, Zhang Nan, Li Shoubang   

  1. Department of Physical Education, Xi’an Shiyou University, Xi’an 710065, Shaanxi Province, China
  • Received:2021-12-13 Accepted:2022-02-15 Online:2023-03-18 Published:2022-07-27
  • Contact: Li Shoubang, PhD, Professor, Master’s supervisor, Department of Physical Education, Xi’an Shiyou University, Xi’an 710065, Shaanxi Province, China
  • About author:Ruan Ling, PhD, Associate professor, Department of Physical Education, Xi’an Shiyou University, Xi’an 710065, Shaanxi Province, China
  • Supported by:
    Shaanxi Natural Science Basic Research Program, No. 2021JQ-586 (to RL)

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摘要：

文题释义：
脂代谢紊乱：是指先天性或获得性因素造成的血液及其他组织器官中脂质(脂类)及其代谢产物质和量的异常。
氧化应激：是指体内氧化与抗氧化作用失衡的一种状态，倾向于氧化，导致中性粒细胞炎性浸润，蛋白酶分泌增加，产生大量氧化中间产物。氧化会产生自由基，自由基会对细胞造成伤害，当自由基产生过多时，就会发生氧化应激。

背景：肥胖是一种多因素的慢性疾病，经常运动可以改善肥胖群体脂代谢紊乱和氧化应激的现象，但不同强度运动对脂肪代谢的具体机制尚不清楚。
目的：探讨不同强度运动对肥胖大鼠脂代谢和氧化应激等指标的影响，并进一步阐明过氧化物酶体增殖物激活受体α、肝X受体α及相关通路蛋白与脂代谢指标的相关性及具体作用机制。
方法：将大鼠随机分为5组：即正常对照组(n=10)、肥胖模型组(n=10)、小强度运动组(n=10)、中等强度运动组(n=9)和大强度运动组(n=8)，正常对照组给予普通饲料，后4组均喂饲高脂饮食，喂养16周制备肥胖大鼠模型，造模成功后各运动组每周运动5 d，每次1 h。运动6周后测试大鼠体质量、肝质量、心脏质量、体脂率、血脂、脂酶和氧化应激指标，脂肪组织病理切片观察脂肪细胞的形态和结构；用Western blot法测定肝脏中载脂蛋白A5、过氧化物酶体增殖物激活受体α、固醇调节元件结合蛋白1c和肝X受体α的蛋白表达水平。
结果与结论：①与肥胖模型组相比，中等强度运动显著降低肥胖大鼠三酰甘油、低密度脂蛋白和游离脂肪酸水平(P < 0.01)，同时显著升高脂蛋白脂酶和肝脂酶活性(P < 0.05)；②各运动组中血浆丙二醛水平显著降低(P < 0.01)，而过氧化氢酶活性显著升高(P < 0.01)；③各运动组载脂蛋白A5蛋白表达水平均显著升高(P < 0.05，P < 0.01，P < 0.01)，固醇调节元件结合蛋白1c和肝X受体α的表达均降低(P < 0.01)，而过氧化物酶体增殖物激活受体α水平仅在中等强度运动组显著升高(P < 0.05)；④与小强度运动组和大强度运动组相比，中等强度运动组载脂蛋白A5水平显著升高(P < 0.01，P < 0.05)；而与小强度运动组相比，中强度运动组固醇调节元件结合蛋白1c表达水平显著降低(P < 0.01)；⑤提示不同强度运动对肥胖大鼠脂代谢相关指标和氧化应激指标均有改善，中等强度运动依赖过氧化物酶体增殖物激活受体α和肝X受体α信号通路的双重调节，改善效果更优。
缩略语：过氧化物酶体增殖物激活受体α：peroxisome proliferators-activated receptors α，PPARα；固醇调节元件结合蛋白1c：Sterol regulatory element-binding protein 1c，SREBP-1c

https://orcid.org/0000-0002-6338-8052(阮凌) ；https://orcid.org/0000-0003-2413-9632(李寿邦)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 运动, 肥胖, 运动强度, 高脂饮食, 脂代谢, 氧化应激, 动物模型

Abstract: BACKGROUND: Obesity is a multifactorial chronic disease. Regular exercise can improve lipid metabolism disorders and oxidative stress in obese groups, but the specific exercise intensity is unclear.
OBJECTIVE: To explore the effect of different-intensity exercises on lipid metabolism and oxidative stress in obese rats and to clarify the correlation of peroxisome proliferators-activated receptor α, liver X receptor α and related pathway proteins with lipid metabolism as well as the specific mechanism.
METHODS: Rats were randomly divided into five groups: normal control group (n=10), obesity model group (n=10), low-intensity exercise group (n=10), moderate-intensity exercise group (n=9) and high-intensity exercise group (n=8). The normal control group was given ordinary feed, and the other four groups were fed with high-fat diet for 16 weeks to prepare the obese rat model. After modelling, rats in each group were arranged to run on the treadmill 1 hour per day and 5 days per week. After 6 weeks of training, body mass, liver mass, heart mass, and body fat percentage, lipids, lipases and oxidative stress were tested, and adipose tissue were cut into sections for cell morphological and structural observations. The protein expression of apolipoprotein A5, peroxisome proliferators-activated receptor α, sterol regulatory element-binding protein 1c and liver X receptor α in liver tissue were detected by western blot assay. 
RESULTS AND CONCLUSION: Compared with the obesity model group, the levels of triacylglycerols, low-density lipoproteins, and free fatty acids were significantly reduced, while the activities of lipoprotein lipase and hepatic lipase increased significantly in the moderate-intensity exercise group (P < 0.01). Plasma malondialdehyde level showed a significant reduction in each exercise group (P < 0.01), but the activity of catalase increased significantly in all exercise groups (P < 0.01). Compared with the obesity model group, the protein expression of apolipoprotein A5 was significantly increased in each exercise group (P < 0.05, P < 0.01, P < 0.01) and the expression levels of sterol regulatory element-binding protein 1c (P < 0.01) and liver X receptor α (P < 0.01) were both decreased, whereas the expression of peroxisome proliferators-activated receptor α only increased significantly in the moderate-intensity exercise group (P < 0.05). Compared with the low- and high-intensity exercise groups, the expression of apolipoprotein A5 was significantly increased in the moderate-intensity exercise group (P < 0.01, P < 0.05). Compared with the low-intensity exercise group, the expression of sterol regulatory element-binding protein 1c was significantly lower in the moderate-intensity exercise group (P < 0.01). To conclude, lipid metabolism disorder and oxidative stress can be improved after different-intensity exercises. The moderate-intensity exercise has better effects, which may be related to the double regulation of exercise-dependent peroxisome proliferators-activated receptor α and liver X receptor α signaling pathways.

Key words: exercise, obesity, exercise intensity, high-fat diet, lipid metabolism, oxidative stress, animal model

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