中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2847-2850.doi: 10.3969/j.issn.1673-8225.2011.15.045

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    

人工合成多肽FGL对神经细胞模型PC12细胞增殖与凋亡的影响

付洪龙，马学晓，于腾波，陈伯华，李  宁   

  1. 青岛大学医学院附属医院，山东省青岛市  266003
  • 收稿日期:2010-12-01 修回日期:2011-02-25 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 陈伯华，硕士，主任医师，青岛大学医学院附属医院，山东省青岛市 266003
  • 作者简介:付洪龙★，男，1984年生，山东省平度市人，汉族，青岛大学医学院在读硕士，主要从事脊髓损伤方面的研究。
  • 基金资助:

    山东省青年科学家研究奖励基金(2007BS03047)，人工合成多肽FGL和IKVAV促进损伤脊髓功能恢复的实验研究；青岛市科技发展计划(06-2-2-6 nsh-2)，FGL多肽促进脊髓损伤后功能恢复的实验研究。

Effects of synthetic peptides FG loop on PC12 cells proliferation and apoptosis

Fu Hong-long, Ma Xue-xiao, Yu Teng-bo, Chen Bo-hua, Li Ning   

  1. Affiliated Hospital of Qingdao University Medical College, Qingdao  266003, Shandong Province, China
  • Received:2010-12-01 Revised:2011-02-25 Online:2011-04-09 Published:2013-11-06
  • Contact: Chen Bo-hua, Master, Chief physician, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China bohuachen@hotmail.com
  • About author:Fu Hong-long★, Studying for master’s degree, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China fuhonglong@126. com
  • Supported by:

    Research Award Fund for Young Scientists in Shandong Province, No. 2007BS03047*; Science and Technology Development Plan of Qingdao City, No. 06-2-2-6 nsh-2*

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摘要：

背景: FGL是NCAM的核心活性多肽片段，可直接作用于成纤维细胞生长因子受体1，激活NCAM的信号传导途径。
目的：观察FGL人工合成多肽联合培养对PC12细胞增殖和凋亡的作用。
方法：将培养的PC12细胞分为对照组和实验组，实验组预先加入1%的FGL多肽溶液。分别于培养1，3，5，7，9 d采用细胞计数试剂8法检测细胞增殖情况。将PC12细胞分为正常组、实验组和损伤组，损伤组加入H2O2刺激16 h。实验组加入H2O2与FGL人工合成多肽刺激16 h，流式细胞仪检测细胞凋亡，荧光定量PCR法检测PC12中的核转录因子κB mRNA表达。
结果与结论：FGL人工合成多肽与PC12复合培养细胞生长良好，可明显促进PC12细胞的活性并且减低PC12 细胞凋亡并可明显降低凋亡模型中PC12细胞核转录因子κB基因的表达。说明FGL多肽可以明显促进PC12细胞增殖，并可以抑制PC12细胞凋亡。

关键词: PC12细胞, FGL多肽, 细胞增殖, 细胞凋亡, 核转录因子&kappa, B

Abstract:

BACKGROUND: FG loop (FGL) is a core active peptide fragment of neural cell adhesion molecule (NCAM), which can directly act on fibroblast growth factor receptor 1 (FGFR1) to activate NCAM signal pathway.
OBJECTIVE: To observe the effects of synthetic peptides FGL on PC12 cells proliferation and apoptosis.
METHODS: ①PC12 cells proliferation and apoptosis: The cultured PC12 cells were divided into control group and experiment group. The experimental group was added with 1% FGL peptide solution. The control group was pre-coated with poly-lysine plates. The cells were cultured 1, 3, 5, 7, 9 d respectively to detect cell proliferation by using Cell Counting Kit-8. ②PC12 apoptosis and nuclear factor kappa B mRNA detection: The PC12 cells were divided into normal group, experimental group and injury group. H2O2 was added into the injury group for 16 hours stimulation. In the experimental group, H2O2 and FGL were used for 16 hours stimulation. The cell apoptosis were detected by flow cytometry; mRNA expression of nuclear factor kappa B was detected by quantitative fluorescent polymerase chain reaction.
RESULTS AND CONCLUSION: PC12 cells cocultured with FGL peptide grow well, which indicates that FGL peptides can promote PC12 cell proliferation and inhibit PC12 cell apoptosis, as well as decrease mRNA expression of nuclear factor kappa B.

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