中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2842-2846.doi: 10.3969/j.issn.1673-8225.2011.15.044

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

放射性咀嚼肌损伤模型软组织病理改变及转化生长因子β1的表达

沈宗泽1，郑建金2，贾暮云3，董  刚2，李  涛2   

  1. 1青岛大学医学院，山东省青岛市  266067
    2青岛市市立医院口腔科，山东省青岛市  266011
    3青岛大学医学院附属医院口腔颌面外科，山东省青岛市  266002
  • 收稿日期:2010-12-20 修回日期:2011-02-19 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 郑建金，硕士，主任医师，教授，硕士生导师，青岛市市立医院口腔科，山东省青岛市 266011
  • 作者简介:沈宗泽★，男，1985年生，中国台湾人，汉族，青岛大学在读硕士，主要从事口腔颌面外科学放射性损伤修复研究。
  • 基金资助:

    青岛市科技局重点基金项目(08-2-1-5-nsh)。

Pathological changes of soft tissues and transforming growth factor beta 1 expression in radiation masseter injury models

Shen Zong-ze1, Zheng Jian-jin2, Jia Mu-yun3, Dong Gang2, Li Tao2   

  1. 1Medical College of Qingdao University, Qingdao  266067, Shandong Province, China
    2Department of Stomatology, Qingdao Municipal Hospital, Qingdao  266011, Shandong Province, China
    3Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Medical College of Qingdao University, Qingdao  266002, Shandong Province, China
  • Received:2010-12-20 Revised:2011-02-19 Online:2011-04-09 Published:2013-11-06
  • Contact: Zheng Jian-jin, Master, Chief physician, Professor, Master’s supervisor, Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China
  • About author:Shen Zong-ze★, Studying for master’s degree, Medical College of Qingdao University, Qingdao 266067, Shandong Province, China johnnyshen886@ live.cn
  • Supported by:

    the Key Foundation of Science and Technology Bureau of Qingdao, No. 08-2-1-5-nsh*

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摘要：

背景： 长期的放射线累积更可引起周围组织纤维化，张口受限，严重影响肿瘤患者的生活品质。
目的：观察大鼠咀嚼肌放射损伤后软组织的病理改变及体内转化生长因子β1 mRNA的表达。
方法：成年Wistar大鼠30只，随机分成对照组(n=10)和放射组(n=20)。放射组利用直线加速器累积照射咀嚼肌40 Gy。光镜和电镜下观察咀嚼肌放射损伤区血管病理改变及RT-PCR检测咀嚼肌放射损伤区转化生长因子β1基因表达。
结果与结论：放射组咀嚼肌放射损伤区毛细血管密度较对照组明显降低(P < 0.01)，转化生长因子β1 mRNA水平较对照组显著升高(P < 0.01)。提示放射损伤可促使组织纤维化修复放射损伤。

关键词: 放射损伤, 大鼠, 毛细血管损伤, 转化生长因子&beta, 1, 血管密度

Abstract:

BACKGROUND: Long-term radioactive ray accumulation leads to surrounding tissues fibrosis accompanied by limitation of mouth-opening, which seriously affects life quality of tumor patients.
OBJECTIVE: To observe pathological change of soft tissues in rats with radiation masseter injury and the level of transforming growth factor β1 (TGF-β1) mRNA expression in vivo.
METHODS: A total of 30 Wistar rats were randomly divided into control group (n=10) and radiated group (n=20). Radiated group were radiated with linear accelerator at a dose of 40 Gy. The pathological changes of vessels were observed under light microscope and electron microscope and expression of TGF-β1 was detected by RT-PCR.
RESULTS AND CONCLUSION: The microvessel density of radiated group was obviously decreased than that of the control group (P < 0.01), but the mRNA expression of was higher than that in the control group (P < 0.01). It suggests that radiated injury can induce fibrosis to repair the radiated injury.

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