中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (14): 2495-2499.doi: 10.3969/j.issn.1673-8225.2011.14.006

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

白细胞介素6体外诱导骨髓间充质干细胞向肝细胞的分化

宋丽华1，王庸晋2，王金胜3，黄  燕2，武翠玲2，石变华2   

  1. 长治医学院，1药理学教研室，2心血管研究所，3病理学教研室，山西省长治市 046000
  • 收稿日期:2010-11-21 修回日期:2010-12-15 出版日期:2011-04-02 发布日期:2013-11-02
  • 作者简介:宋丽华★，女，1970年生，辽宁省盖州市人，汉族，2003年中南大学湘雅医学院毕业，硕士，副教授，主要从事植物雌激素与干细胞的定向分化研究。 slh10282001@yahoo.com.cn
  • 基金资助:

    山西省高校科技研究开发项目(200611034)，长治医学院科研发展基金项目(200715)。

Interleukin 6 induces the differentiation of bone marrow mesenchymal stem cells into hepatocytes in vitro

Song Li-hua1, Wang Yong-jin2, Wang Jin-sheng3, Huang Yan2, Wu Cui-ling2, Shi Bian-hua2   

  1. 1Department of Pharmacology, 2Angiocardiopathy Institute, 3Department of Pathematology, Changzhi Medical College, Changzhi 046000, Shanxi Province, China
  • Received:2010-11-21 Revised:2010-12-15 Online:2011-04-02 Published:2013-11-02
  • About author:Song Li-hua★, Master, Associate professor, Department of Pharmacology, Changzhi Medical College, Changzhi 046000, Shanxi Province, China slh10282001@yahoo.com.cn
  • Supported by:

     the Science and Technology Research Development Project of Higher Learning School of Shanxi Province, No. 200611034*; the Scientific Research Development Foundation of Changzhi Medical College, No. 200715*

PDF

507

被引次数

8

摘要：

背景：骨髓间充质干细胞是肝细胞的重要肝外来源，在多种细胞因子和生长因子诱导下可分化为肝细胞，从而参与肝功能的修复和重建。
目的：观察白细胞介素6是否可诱导骨髓间充质干细胞向肝细胞定向分化。
方法：采用贴壁法分离培养昆明种雄性小鼠骨髓间充质干细胞，在无血清肝细胞培养液HEPATOZYME-SFM中分别加入2.5，5，10，20 µg/L白细胞介素6诱导其向肝样细胞分化；诱导培养0，7，14，21，28 d时取出细胞爬片，细胞免疫组织化学法检测细胞角蛋白18、甲胎蛋白表达情况，过碘酸-Schiff糖原染色检测细胞功能，ELISA和硝酸还原酶法分别检测培养液中白蛋白和一氧化氮水平。
结果与结论：当白细胞介素6质量浓度在2.5~10 µg/L范围内，呈浓度依赖性和时间依赖性增加细胞角蛋白18、糖原表达率及细胞培养液中白蛋白水平；甲胎蛋白表达为先升后降直至21d停止。当白细胞介素6质量浓度增加到20 µg/L时上述诱导作用均不及质量浓度10 µg/L组。只有10 µg/L白细胞介素6组诱导到28 d时细胞培养液中测得微量一氧化氮；提示白细胞介素6可诱导骨髓间充质干细胞向肝系细胞分化，且在10 µg/L作用最强，质量浓度再增高时诱导作用反而减弱。

关键词: 骨髓间充质干细胞, 白细胞介素6, 分化, 肝细胞, 干细胞培养与分化

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are an important source of hepatocytes. BMSCs can differentiate into hepatocytes under the induction of multiple cytokines and growth factors, and participate in repair and reconstruction of liver function.
OBJECTIVE: To explore the effect of interleukin 6 on the differentiation of BMSCs into hepatocytes.
METHODS: BMSCs were isolated from Kunming male mice and cultured by attachment method and treated with interleukin 6 (2.5, 5, 10, 20 µg/L) in HEPATOZYME-SFM nutrient solution to induce the BMSCs differentiation into hepatocytes. On days 0, 7, 14, 21, 28 after induction, the expressions of cytokeratin 18, alpha-fetoprotein and glycogen were identified by immunohistochemical method and periodic acid-schiff. The contents of albumin and nitric oxide in the liquid supernatant were identified by ELISA and nitrate reductase method, respectively.
RESULTS AND CONCLUSION: Interleukin 6 resulted in dose-dependent (2.5, 5, 10 µg/L) and time-dependent increase in the expressions of cytokeratin 18 and glycogen and the content of albumin. The expression of alpha-fetoprotein increased then decreased to disappearance on day 21. The above effects of interleukin 6 (20 µg/L) was weaker than that of interleukin 6 (10 µg/L). The content of small amount nitric oxide was detected when interleukin 6 was 10 µg/L on day 28. These demonstrated that interleukin 6 is able to induce the differentiation of BMSCs into hepatocyte-like cells, which shows strongest effects at 10 µg/L. Increased mass concentration showed decreased induction effects.

中图分类号: