中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (12): 2265-2269.doi: 10.3969/j.issn.1673-8225.2011.12.043

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

动物源性骨软骨支架复合骨髓间充质干细胞/软骨细胞修复兔膝关节骨软骨复合缺损

谭文成1，查振刚2，张嘉晴3，郑立恒4，梁耀中2，夏吉生4，黄馨霈1，吴  昊2，林宏生2   

  1. 1澳门医学科技研究协会，澳门特别行政区  999078
    2暨南大学附属第一医院，暨南大学骨科疾病研究所，广东省广州市  510630
    3 暨南大学医学院，广东省广州市  510632
    4 澳门仁伯爵医院，澳门特别行政区  999078
  • 收稿日期:2010-12-12 修回日期:2011-01-18 出版日期:2011-03-19 发布日期:2011-03-19
  • 通讯作者: 林宏生，主任医师，硕士生导师，暨南大学附属第一医院，暨南大学骨科疾病研究所，广东省广州市 510630
  • 作者简介:谭文成☆，男，1976年生，广东省新会市人，汉族，暨南大学在读博士，医师，主要从事骨关节疾病与内外科技术研究。
  • 基金资助:

    澳门医学科技研究基金项目(031/2009/A)，广州市科技计划项目(2008Z1-E411)。

Animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells/chondrocytes for repair of composite osteochondral defects in rabbit knee joints

Tan Wen-cheng1, Zha Zhen-gang2, Zhang Jia-qing3, Zheng Li-heng4, Liang Yao-zhong2, Xia Ji-sheng4, Huang Xin-pei1, Wu Hao2, Lin Hong-sheng2   

  1. 1Macau Medical Science & Technology Research Association, Macao Special Administrative Region  999078, China
    2Institute of Orthopaedic Diseases, the First Affiliated Hospital, Jinan University, Guangzhou  510630, Guangdong Province, China
    3Medical College, Jinan University, Guangzhou  510632, Guangdong Province, China
    4Centro Hospitalar Conde de SÃo Janu?rio, Macao Special Administrative Region  999078, China
  • Received:2010-12-12 Revised:2011-01-18 Online:2011-03-19 Published:2011-03-19
  • Contact: Lin Hong-sheng, Chief physician, Master’s supervisor, Institute of Orthopaedic Diseases, the First Affiliated Hospital, Jinan University, Guangzhou 510630, Guangdong Province, China woohaoo@163.com
  • About author:Tan Wen-cheng☆, Studying for doctorate, Physician, Macau Medical Science & Technology Research Association, Macao Special Administrative Region 999078, China 1002358969@qq.com
  • Supported by:

    the Macau Medical Science & Technology Research Fund Project, No. 031/2009/A*; Technological Planning Projects of Guangzhou, No. 2008Z1-E411*

PDF

566

被引次数

12

摘要：

背景：以往支架材料修复骨软骨的实验大都存在骨软骨耦合界面修复不良的情况。
目的：观察骨髓间充质干细胞/软骨细胞复合动物源性骨软骨支架修复兔膝关节骨软骨复合缺损的可行性。
方法：将新西兰大白兔随机抽签分为实验组、对照组、空白组，制作单侧膝关节骨软骨复合缺损后，实验组于骨缺损处植入自体骨髓间充质干细胞/诱导分化的软骨细胞与同种异体动物源性骨软骨复合支架，对照组于骨缺损处植入同种异体动物源性骨软骨支架、空白组未植入任何材料。术后4，8，12周行大体观察、苏木精-伊红染色、甲苯胺蓝染色。
结果与结论：实验组大体观察见复合缺损区完全修复，局部无凹陷，新生组织和周围组织融合，苏木精-伊红染色和甲苯胺蓝染色见软骨缺损区由新生的透明软骨样组织修复，细胞柱状排列，极性好，软骨陷窝明显，骨缺损区由骨样组织修复，新生软骨和软骨下骨以及宿主骨界面耦合良好，甲苯胺蓝染色阳性率和组织学评分优于对照组、空白组(P < 0.05)。说明诱导分化的自体软骨细胞和骨髓间充质干细胞共培养复合动物源性骨软骨支架所构建的细胞-支架复合体能成功修复兔膝关节软骨和软骨下骨的复合缺损，是一种理想的骨软骨复合缺损修复方法。

关键词: 动物源性, 支架, 骨髓间充质干细胞, 软骨细胞, 修复, 缺损

Abstract:

BACKGROUND: Though there were many experiments addressing repairing osteochondral defects before, faulty restoration occurred at coupling interfaces.   OBJECTIVE: To investigate the feasibility of repairing of osteochondral composite defects in rabbit knees with animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells (BMSCs)/chondrocytes.
METHODS: New Zealand white rabbits were randomly divided into the experimental, control and blank groups and prepared for unilateral knee joint osteochondral defects. Animal-origin osteochondral scaffold combined with BMSCs/chondrocytes, animal-origin osteochondral scaffold and no material was implanted to repair the defects in the experimental, control and blank groups, respectively. Healing condition was evaluated by gross observation, hematoxylin-eosin staining, and toluidine blue staining at 4, 8, and 12 weeks after operation.  
RESULTS AND CONCLUSION: At 12 weeks after operation, gross observation showed the defects were repaired completely without local depression and the regenerated tissues were fused with surrounding tissues in the experimental group. Hematoxylin-eosin staining and toluidine blue staining revealed that there were many new hyaline cartilages in the cartilage defects in which columnar cells were lined well and cartilage lacuna was obviously, also, there were many bony tissues in the bone defects. The regeneration cartilage, the underlying subchondral bone and host bone were coupled completely. The toluidine blue positive rate and histologic scores of the experimental group were superior to those of the control and blank groups (P < 0.05). It is demonstrated that animal-origin osteochondral scaffold combined with BMSCs/chondrocytes is an ideal method to repair defects between cartilage and the underlying subchondral bone.

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