中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 281-285.doi: 10.3969/j.issn.1673-8225.2011.02.022

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

碱性成纤维细胞生长因子联合透明质酸钠对角膜保存液中内皮细胞的作用

杨  月1，2，赵  敏1   

  1. 1重庆医科大学附属第一医院眼科，重庆市  400016
    2重庆市第五人民医院眼科，重庆市   400062
  • 收稿日期:2010-07-10 修回日期:2010-08-13 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 赵敏，教授，重庆医科大学附属第一医院眼科，重庆市 400016 inzhao2002@yahoo.com.cn
  • 作者简介:杨月★，女，1979年生，重庆市人，土家族，2008年重庆医科大学毕业，硕士，主要从事角膜及眼表方面的研究。 yangyue_20049@yahoo.cn
  • 基金资助:

    重庆市卫生局科研资助项目(渝卫科教2008-2-114)，课题名称：改良角膜中期保存液的研制及应用研究。

Effects of basic fibroblast growth factor combined with sodium hyaluronate on endothelial cells in corneal preservation solution

Yang Yue1,2, Zhao Min1   

  1. 1Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
    2Department of Ophthalmology, Chongqing Fifth People’s Hospital, Chongqing  400062, China
  • Received:2010-07-10 Revised:2010-08-13 Online:2011-01-08 Published:2011-01-08
  • Contact: Zhao Min, Professor, Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China inzhao2002@yahoo.com.cn
  • About author:Yang Yue★, Master, Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Ophthalmology, Chongqing Fifth People’s Hospital, Chongqing 400062, China yangyue_20049@yahoo.cn
  • Supported by:

    the Science and Technology Foundation of Health Bureau of Chongqing City, No. 2008-2-114

PDF

383

被引次数

2

摘要：

背景：作者前期将透明质酸钠和碱性成纤维细胞生长因子分别加入基础培养基，制成改良角膜保存液并证实其对角膜内皮细胞活性的保存作用。
目的：探讨碱性成纤维细胞生长因子及透明质酸钠在角膜中期保存液中的作用及其有效浓度，评价该两种物质联合对角膜内皮细胞的保护作用。
方法：在以基础培养基 MEM、M-199为主要成分，添加 6%葡聚糖、HEPES缓冲系、抗生素等制成角膜保存液的基础上，加入不同浓度的透明质酸钠和碱性成纤维细胞生长因子，并设立未加透明质酸钠及碱性成纤维细胞生长因子的对照组。各组于4 ℃下保存新西兰大白兔角膜片，于3，5，7，10，14 d，行裂隙灯检查、锥虫蓝-茜素红染色、角膜组织病理切片及扫描电镜观察检测角膜内皮细胞活性，评价保存质量。 
结果与结论：加入碱性成纤维细胞生长因子及透明质酸钠的中期角膜保存液保存的角膜片其透明度、内皮细胞活性、显微结构、超微结构均明显优于未加组，各实验组同期比较，浓度较高组效果优于浓度较低组。碱性成纤维细胞生长因子及透明质酸钠同时加入基础保存液中能够对角膜内皮细胞进行有效的保护，其效果与浓度有关。

关键词: 碱性成纤维细胞生长因子, 透明质酸钠, 内皮细胞, 中期保存液, 角膜

Abstract:

BACKGROUND: In our previous studies, corneal preservation solution was improved by adding basic fibroblast growth factor (bFGF) combined with sodium hyaluronate, which be confirmed has effect on maintain endothelial cell activity.
OBJECTIVE: To explore effects of bFGF and sodium hyaluronate in corneal preservation solution and effective concentrations, in addition, to evaluate the protect effect of these two materials on corneal endothelial cells.
METHODS: 6% glucosan, HEPES buffer solution and antibiotics were added into MEM and M-199 to prepared corneal preservation solution, additionally, bFGF and sodium hyaluronate with different concentrations were added. Simultaneously, control group without bFGF and sodium hyaluronate was established. Cornea of New-Zealand white rabbits preserved in each group were detected by slit-lamp examination, trypan blue-alizarin red staining, and scanning electron microscope at 3, 5, 7, 10 and 14 days after operation.
RESULTS AND CONCLUSION: The transparency, endothelial cell activity, microstructure, as well as ultrastructure of the improved corneal preservation solution group were obviously better than those of the other group. Compared among the experimental groups, the high concentration group was superior to the low concentration group. The findings demonstrated that bFGF and sodium hyaluronate in corneal preservation solution can protect corneal endothelial cells, and the effectiveness is related to concentration.

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