中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (50): 9346-.doi: 10.3969/j.issn.1673-8225.2010.50.009

• 织构建与生物活性因子 • 上一篇    下一篇

胰岛素样生长因子1和成纤维细胞生长因子7与毛乳头细胞的生长

张国强1，程毅1，李玲2，高顺强1   

  1. 1河北医科大学第四医院皮肤科，河北省石家庄市 050011；2保定市第一中心医院皮肤科，河北省保定市 071000
  • 出版日期:2010-12-10 发布日期:2010-12-10
  • 通讯作者: 高顺强，主任医师、教授，河北医科大学第四医院皮肤科，河北省石家庄市 050011 gaoshunqiang@medmail.com.cn
  • 作者简介:张国强★，男，1977年生，河北省栾城县人，汉族，2003年河北医科大学毕业，硕士，主治医师，主要从事毛囊组织工程及皮肤肿瘤方面的研究。 zgq810328@sina.com
  • 基金资助:

    中华医学会-欧莱雅中国人健康毛发研究项目(H2009030412)，毛乳头细胞生物学特性及其生长因子的相关性研究。

Effects of insulin-like growth factor-1 and fibroblast growth factor-7 on growth of dermal papilla cells

Zhang Guo-qiang1, Cheng Yi1, Li Ling2, Gao Shun-qiang1   

  1. 1 Department of Dermatology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China; 2 Department of Dermatology, First Central Hospital of Baoding, Baoding 071000, Hebei Province, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Gao Shun-qiang, Chief physician, Professor, Department of Dermatology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China gaoshunqiang@medmail.com.cn
  • About author:Zhang Guo-qiang★, Master, Attending physician, Department of Dermatology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China zgq810328@sina.com
  • Supported by:

    Chinese Medical Association-L’OREAL China Skin/Hair Grant, No. H2009030412*

PDF

605

被引次数

22

摘要：

背景：毛乳头细胞凝集性生长与其诱导毛发生长有关。毛乳头细胞分泌的多种生长因子如胰岛素样生长因子1、成纤维细胞生长因子7可能对毛乳头细胞生长具有重要的影响。

目的：观察正常人毛乳头细胞的增长与生长方式与胰岛素样生长因子1、成纤维细胞生长因子7的相关性。

方法：采用二步酶消化法分离培养毛乳头细胞，免疫组化法和流式细胞仪分别检测两种生长因子在凝集性与非凝集性生长的毛乳头细胞中的表达，MTT法检测2.5-100 μg/L质量浓度胰岛素样生长因子1对人毛乳头细胞增殖的影响。

结果与结论：胰岛素样生长因子1与成纤维细胞生长因子7均表达于细胞胞浆，但表达量随时间减弱，其中第3代明显比第9代毛乳头细胞中成纤维细胞生长因子7的表达强烈(P < 0.05)。与对照组相比，不同质量浓度胰岛素样生长因子1均有明显促进毛乳头细胞增殖的作用(P < 0.05)，其中以2.5 μg/L最为显著。提示毛乳头细胞的凝集性生长状态的改变可能与胰岛素样生长因子1、成纤维细胞生长因子7表达下降有关，胰岛素样生长因子1可明显刺激毛乳头细胞增殖。

关键词: 毛囊, 人毛乳头细胞, 胰岛素样生长因子1, 成纤维细胞生长因子7, 细胞培养

Abstract:

BACKGROUND: Agglutinative growth of dermal papilla cells (DPCs) is related to their abilities to induce hair growth. Growth factors secreted by DPCs, such as insulin-like growth factor-1 (IGF-1) and fibroblast growth factor-7 (FGF-7), play an important role in DPCs growth. 
OBJECTIVE: To observe correlation between DPCs proliferation, growth characteristics and IGF-1 and FGF-7.
METHODS: Dermal papillas were isolated from human scalp hair follicles by two-step digestion method. The expressions of IGF-1, FGF-7 protein in different passages of cultured DPCs were detected by immunohistochemical method and flow cytometry. Effects of different concentration of IGF-1 (2.5-100 μg/L) on the cell proliferation of DPCs were measured by MTT.
RESULTS AND CONCLUSION: The result of immunohistochemistry showed that IGF-1 and FGF-7 were expressed in the cytoplasm, which decreased with time prolonged. The expression of IGF-1 in the 3rd generation DPCs was obviously greater than that of the 9th generation (P < 0.05). Compared with the control group, different concentrations of IGF-1 could significantly promote the proliferation of DPCs (P < 0.05), especially in the 2.5 μg/L. The change of the aggregative growth of DPCs may be related to the decrease of the expressions of IGF-1 and FGF-7. IGF-1 can stimulate the proliferation of DPCs significantly.

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