中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9208-.doi: 10.3969/j.issn.1673-8225.2010.49.020

• 干细胞因子及调控因子 • 上一篇    下一篇

树突状细胞-细胞因子诱导杀伤细胞体外抗白血病K562细胞的效应

宋盈盈，苏荣英，艾丽梅   

  1. 辽宁医学院附属第一医院血液科，辽宁省锦州市 121001
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 艾丽梅，主任医师，辽宁医学院附属第一医院血液科，辽宁省锦州市 121001 alm121001@yahoo.com.cn
  • 作者简介:宋盈盈★，女，1985年生，山东省临沂市人，汉族，辽宁医学院在读硕士，主要从事血液肿瘤的诊断和治疗研究。
  • 基金资助:

    辽宁省教育厅科研项目(2008390)“免疫效应细胞逆转血液肿瘤耐药”和辽宁医学院博士启动基金(2009)项目“DC-CIK抗淋巴瘤免疫效应机制研究”。

Effects of dendritic cells-cytokine-induced killer cells against leukemia K562 cell lines in vitro

Song Ying-ying, Su Rong-ying, Ai Li-mei   

  1. Department of Hematology, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Ai Li-mei, Chief physician, Department of Hematology, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China alm121001@yahoo.com.cn
  • About author:Song Ying-ying★, Studying for master’s degree, Department of Hematology, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Supported by:

    the Scientific Research Program of Department of Education of Liaoning Province, No. 2008390*; the Doctor Priming Foundation of Liaoning Medical University in 2009*

PDF

504

被引次数

10

摘要：

背景：将细胞因子诱导的杀伤细胞与树突状细胞联合起来治疗恶性肿瘤，将有助于解除部分肿瘤患者T细胞的免疫无能，从而发挥协同抗肿瘤作用。
目的：观察细胞因子诱导的杀伤细胞与树突状细胞共同培养对体外抗白血病K562细胞株的效应。
方法：分离正常人外周血单个核细胞，诱导成树突状细胞和细胞因子诱导的杀伤细胞。将树突状细胞和细胞因子诱导的杀伤细胞共同培养3 d作为效应细胞，流式细胞术检测细胞表型；以K562为靶细胞，分别以单个核细胞，细胞因子诱导的杀伤细胞，树突状细胞和树突状细胞-细胞因子诱导的杀伤细胞为效应细胞，采用MTT法进行体外杀伤实验。
结果与结论：随着效靶比的增加，各组效应细胞对K562细胞的杀伤活性也增加；同一效靶比下，各组效应细胞对K562细胞的杀伤活性不同，其中树突状细胞-细胞因子诱导的杀伤细胞对肿瘤细胞的杀伤活性最强，可达(77.88±1.57)%(P < 0.01)。提示树突状细胞-细胞因子诱导的杀伤细胞抗白血病细胞的作用显著，较单纯应用细胞因子诱导的杀伤细胞或树突状细胞具有更强的抗肿瘤活性。

关键词: 细胞因子诱导的杀伤细胞, 树突状细胞, 效应, 白血病K562细胞, 免疫治疗

Abstract:

BACKGROUND: The combined application of cytokine-induced killer cells (CIK) and dendritic cells to treatment of malignant tumors will help to remove T cell incompetence of some cancer patients, and plays synergistic anti-tumor effects.
OBJECTIVE: To observe the effect of CIK cells co-cultured with dendritic cells against K562 cell lines.
METHODS: CIK and dendritic cells were induced by peripheral blood mononuclear cells of normal persons. Dendritic cells and CIK were co-cultured for 3 days as effector cells. Immunophenotype of cells was measured by flow cytometry. K562 served as target cells, and peripheral blood mononuclear cells, CIK, dendritic cells, dendritic cells-CIK as effector cells. The killing activity was assayed by MTT assay.
RESULTS AND CONCLUSION: With the increased ratio of effector to target, effector killing activity of each group also increased; for the same ratio, the killing activity of each group was different, of which dendritic cells-CIK was the strongest, up to (77.88± 1.57)% (P < 0.01). It also showed that dendritic cells-CIK has a stronger anti-tumor activity, and the toxicity of dendritic cells-CIK against leukemia cells was significantly higher than simple application of CIK or dendritic cells.

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