中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8631-.doi: 10.3969/j.issn.1673-8225.2010.46.019

• 组织构建与中医药 • 上一篇    下一篇

外伤性膝关节僵硬模型兔中药熏洗治疗后内侧副韧带组织中糖胺聚糖的表达

王锋，徐勇，夏云建   

  1. 武汉商业服务学院体育系，湖北省武汉市  430056
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 作者简介:王锋★，男，1977年生，湖北省当阳市人，汉族，2004年武汉体育学院毕业，硕士，讲师，主要从事体育教育与运动训练医务监督方面的研究。 trainer-wong@yahoo.com.cn

Glycosaminoglycan expression in medial collateral ligaments of knee traumatic stiff rabbits following traditional Chinese medicine fumigation therapy

Wang Feng, Xu Yong, Xia Yun-jian   

  1. Department of Physical Education, Wuhan Commercial Service College, Wuhan  430056, Hubei Province, China
  • Online:2010-11-12 Published:2010-11-12
  • About author:Wang Feng★, Master, Lecturer, Department of Physical Education, Wuhan Commercial Service College, Wuhan 430056, Hubei Province, China trainer-wong@yahoo.com.cn

PDF

292

摘要：

背景：中医药治疗膝关节外伤性僵硬取得了较好的疗效，但其机制尚不清楚。
目的：构建膝关节外伤性僵硬模型兔，探讨伤科熏洗汤治疗外伤性膝关节僵硬的可能机制。
方法：向24只兔右膝关节腔内注射自身血液后经管型石膏伸直位固定7周制备兔膝关节外伤性僵硬模型。中药组用伤科熏洗汤局部熏洗膝关节，水熏组用热水熏洗，模型组不做处理。2周后，测定兔血液中丙氨酸氨基转移酶、尿素氮、肌酐及膝内侧副韧带组织中糖胺聚糖水平，同时观察治疗前后兔右膝关节活动度及患侧膝关节的病理改变。
结果与结论：造模7周后，所有动物右膝关节活动度较对侧明显降低(P < 0.01)。伤科熏洗汤治疗2周后，右膝关节活动度无明显增加，关节存在关节囊增厚，新生胶原纤维排列紊乱，滑膜结构消失等病理改变。血液中丙氨酸氨基转移酶、尿素氮、肌酐水平与水熏组、模型组比较差异无显著性意义(P > 0.05)，但膝内侧副韧带组织中糖胺聚糖水平明显增加(P < 0.01)。说明伤科熏洗汤可提高韧带组织中糖胺聚糖水平，对膝关节外伤性僵硬有预防和辅助治疗作用。

关键词: 膝关节, 外伤性僵硬, 伤科熏洗汤, 糖胺聚糖, 兔

Abstract:

BACKGROUND: Traditional Chinese medicine fumigation therapy has shown good curative effects in the treatment of knee traumatic stiff, but the mechanism remains unclear.
OBJECTIVE: To construct a traumatic stiff knee model, and to explore the mechanism of Shangkexungxitang (SKXXT) for treating traumatic stiff knee.
METHODS: Totally 24 rabbits were prepared for traumatic stiff knee models by injecting one’s own blood into right knee joint and immobilized the knee in extension position with plaster for 7 weeks. The traumatic stiff knees were steamed and washed with SKXXT or hot water in the Chinese medicine group and water control group. There was no treatment in the model group. The level of serum alanine transaminase, blood urea nitrogen, creatinine, as well as glycosaminoglycan content was measured at 2 weeks after treatment. At the same time, the range of motion (ROM) of the knee before and after therapeutic period and pathological changes of affected sides were observed. 
RESULTS AND CONCLUSION: After 7 weeks of immobilization, the ROM of right knee joints were significantly reduced (P < 0.01). No obvious change of ROM happened, but the articular capsule became thickening, collagen fiber arranged disorderly, and membrane structure disappeared at 2 weeks after treatment. There were no significances of the alanine transaminase, blood urea nitrogen and creatinine levels in three groups (P > 0.05). But the content of glycosaminoglycan in Chinese medicine group was much greater (P < 0.01). SKXXT can enhance glycosaminoglycan content in ligaments, which has an effect of prevention and auxiliary therapy on traumatic stiff knee.

中图分类号: