中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8635-.doi: 10.3969/j.issn.1673-8225.2010.46.020

• 组织构建与中医药 • 上一篇    下一篇

电针“后三里”穴位促进血中丹参酮ⅡA的吸收

李忠汗1，黄  仑2，刘力维1，鲁  佳1，喻  斌2，朱 毅1，徐  斌1，方泰惠2   

  1. 南京中医药大学，1针灸结合药物实验室，针药结合省部共建教育部重点实验室；2药理药效实验室，江苏省中药药效和安全性评价重点实验室，江苏省南京市  210046
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 徐斌，男，研究员，教授，硕士生导师，南京中医药大学针灸结合药物实验室，针药结合省部共建教育部重点实验室，江苏省南京市 210046 xuuuux@sina.com 并列通讯作者：方泰惠，教授，博士生导师，南京中医药大学药理药效实验室，江苏省中药药效和安全性评价重点实验室， 江苏省南京市 210046 fangtaihui@sina.com
  • 作者简介:李忠汗，男，1988年生，汉族，主要从事针药结合效应及其机制的研究。anyedejingling@gmail.com
  • 基金资助:

    江苏省高等学校大学生实践创新计划项目资助(00396)，课题名称：针刺对丹参脂溶性有效成分在小鼠体内吸收情况影响的实验研究。

Electro-acupuncture at Housanli (ST36) promotes the absorption of tanshinoneⅡA

Li Zhong-han1, Huang Lun2, Liu Li-wei1, Lu Jia1, Yu Bin2, Zhu Yi1, Xu Bin1, Fang Tai-hui2   

  1. 1Key Provincial-level Laboratory of Integrative Acupuncture and Drugs, Ministry of Education, Nanjing University of Traditional Chinese Medicine, Nanjing  210029, Jiangsu Province, China; 2Jiangsu Provincial Key Laboratory of the Evaluation of Efficacy and Safety, Nanjing University of Traditional Chinese Medicine, Nanjing  210029, Jiangsu Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: u Bin, Researcher, Professor, Master’s supervisor, Key Provincial-level Laboratory of Integrative Acupuncture and Drugs, Ministry of Education, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China xuuuux@sina.com Fang Tai-hui, Professor, Doctoral supervisor, Jiangsu Provincial Key Laboratory of the Evaluation of Efficacy and Safety, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China fangtaihui@sina.com
  • About author:Li Zhong-han, Key Provincial-level Laboratory of Integrative Acupuncture and Drugs, Ministry of Education, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China anyedejingling@gmail.com
  • Supported by:

    Students Practice Innovative Projects for Colleges and Universities in Jiangsu Province, No. 00396*

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摘要：

背景：近年来大量研究表明针药结合对心肌缺血的改善存在协同作用，但对其作用机制尚未阐明。
目的：观察电针“后三里”穴位结合不同浓度丹参预防心肌缺血的效应及丹参酮ⅡA在血液和心肌中的水平，探究针药结合协同作用的可能途径。
方法：将SD大鼠随机分为假手术组，模型组，低、高剂量药物组，低、高剂量药物+针刺组。低、高剂量药物组分别用0.1和0.2 mg/L的丹参煎剂灌胃。针刺组电针“后三里”穴位。观察大鼠灌胃丹参后心电图T波、心肌和血清中乳酸脱氢酶和肌酸激酶的变化，用高效液相色谱法法观测丹参酮ⅡA在血清及心脏组织中的质量浓度。
结果与结论：电针结合不同浓度的丹参可以降低心电图T波及乳酸脱氢酶和肌酸激酶水平，说明对心肌缺血所致的损伤有保护作用。相同时间内丹参酮ⅡA在低剂量药物+针刺组大鼠血清样品中明显高于低剂量组(P < 0.05)，而在心脏组织匀浆样品与低剂量组相比无明显差异(P > 0.05)，说明电针在药物浓度较低时可以起到促进药物吸收的作用，主要表现在促进血药浓度的提高，但并不增加靶器官上的药物浓度。由此可知，电针“后三里”穴位对丹参酮ⅡA吸收有促进作用，丹参煎剂配合电针刺激能显著提高丹参酮ⅡA在血中的药物浓度。

关键词: 丹参酮ⅡA, 电针, 针药结合, 心肌缺血, 后三里, 组织构建

Abstract:

BACKGROUND: In recent years, a large number of studies have shown that acupuncture combined with medication can synergy to improve myocardial ischemia, but the mechanism of this treatment has not been clarified.
OBJECTIVE: To observe the effects of electro-acupuncture of Housanli (ST36) in combination with oral doses of Salvia (varying concentrations) in the prevention of myocardial ischemia, and to observe the levels of tanshinone ⅡA in the blood and in myocardial muscle tissues after treatment.
METHODS: SD rats were randomly divided into the sham-surgery, model, low- and high- dose drug, and low- and high- dose drug + acupuncture groups. Electro-acupuncture was given on Housanli (ST36) in the adult SD rats following intragastric administration of 0.1 or 0.2 mg/L Danshen decoction. The changes of electrocardiogram T wave, heart and serum lactate dehydrogenase (LDH), and creatine kinase (CK) were observed after intragastric administration. HPLC was used for observation of tanshinone ⅡA concentrations in serum and cardiac tissues.
RESULTS AND CONCLUSION: Electro-acupuncture combined with different concentrations of Dansen decoction could protect the damage caused by myocardial ischemia by reducing the level of T wave and related enzymes. The content of Tanshinone ⅡA in the blood in the low dose and acupuncture group was higher than that in the low dose group in the same period (P < 0.05), but the content of Tanshinone ⅡA in the heart tissue in the low-dose + acupuncture group was not higher than that in the low-dose group (P > 0.05). Electro-acupuncture can promote the drug absorption in the low drug concentration, this role is mainly to promote the improvement of blood concentration, but does not increase the drug concentration on the target organ. Electro-acupuncture can promote drug absorption, thus, increasing the efficacy of the drug.

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