中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8435-8440.doi: 10.3969/j.issn.1673-8225.2010.45.018

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

自体骨髓间充质干细胞移植急性心肌梗死犬血管内皮生长因子及血管新生的变化　

王  永1，吴继雄2，方五旺1，林丙来1，芮祖琴1   

  1. 1芜湖市第二人民医院心内科二科，安徽省芜湖市  241000；2安徽医科大学第一附属医院心内科，安徽省合肥市 230022
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 方五旺，博士，主任医师，主要从事心血管疾病的介入治疗研究。 fangwuwang@sina.com
  • 作者简介:王永★，男，1977年生，安徽省亳州市人，汉族，2008年安徽医科大学心血管内科专业毕业，硕士，主治医师，主要从事冠心病的诊治研究。 hongpingguo2046@sina.com
  • 基金资助:

    安徽省教育厅自然科学重点科研项目（2006KJ088A）。项目名称:骨髓干细胞移植治疗急性心肌梗死的实验研究。

Changes in vascular endothelial growth factor and angiogenesis in dogs with acute myocardial infarction following autologous bone marrow mesenchymal stem cells transplantation

Wang Yong1, Wu Ji-xiong2, Fang Wu-wang1, Lin Bing-lai1, Rui Zu-qin1   

  1. 1 Second Department of Cardiology, Second People’s Hospital of Wuhu, Wuhu  241000, Anhui Province, China;2 Department of Cardiology, First Affiliated Hospital, Anhui Medical University, Hefei  230022, Anhui Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Fang Wu-wang, Doctor, Chief physician, Second Department of Cardiology, Second People’s Hospital of Wuhu, Wuhu 241000, Anhui Province, China fangwuwang@sina.com
  • About author:Wang Yong★, Master, Attending physician, Second Department of Cardiology, Second People’s Hospital of Wuhu, Wuhu 241000, Anhui Province, China hongpingguo2046@sina.com
  • Supported by:

     the Key Scientific Research Program of Natural Science of Department of Education of Anhui Province, No. 2006KJ088A*

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摘要：

背景：研究表明骨髓间充质干细胞在体内外可诱导分化为心肌样细胞，局部移植骨髓间充质干细胞可以促进血管新生，阻止心室重构，改善心功能，但具体机制尚未阐明。
目的：探讨骨髓间充质干细胞心肌移植后犬体内血管内皮生长因子的表达变化以及对心肌梗死区血管新生的影响。
方法：健康杂种犬随机分成实验组和对照组，均建立急性心肌梗死模型。建模后一二小时，实验组犬将BrdU标记的骨髓间充质干细胞悬液注入心肌梗死区四五个不同部位，对照组注入等量的DMEM。骨髓间充质干细胞移植4周后，酶联免疫吸附测定法检测血清中的血管内皮生长因子浓度，并采用免疫组化染色法进行毛细血管密度测定。
结果与结论：移植后第1天及1，2，3，4周血管内皮生长因子的浓度实验组与对照组相比均增加(P < 0.01)，且两组血管内皮生长因子的浓度均随时间呈下降趋势(P < 0.01)；心肌梗死区单位面积毛细血管数实验组较对照组明显增加(P < 0.01)。结果表明心肌梗死区移植骨髓间充质干细胞后使得体内血管内皮生长因子的浓度增加，并可促进心肌梗死后毛细血管新生。

关键词: 急性心肌梗死, 骨髓间充质干细胞, 移植, 血管内皮生长因子, 血管新生

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into cardiomyocyte-like cells in vivo and in vitro. BMSCs transplantation in local region can promote angiogenesis, prevent ventricle reconstruction, and improve cardiac function, but precise mechanisms remain unclear.
OBJECTIVE: To investigate vascular endothelial growth factor (VEGF) expression and angiogenesis in myocardial infarction zone following BMSCs cardiac transplantation in dogs.
METHODS: Healthy hybrid dogs were randomly assigned to experimental and control groups. All dogs were used to establish models of acute myocardial infarction. At 1 and 2 hours following model induction, BrdU-labeled BMSC suspension was infused into four or five regions of myocardial infarction region in the experimental group, whereas an equal volume of Dulbecco’s modified Eagle’s medium was injected into the dogs of control group. At 4 weeks following transplantation, VEGF concentration in serum was determined using enzyme-linked immunosorbent assay (ELISA). Capillary density was detected using immunohistochemical staining.
RESULTS AND CONCLUSION: On day 1, weeks 1, 2, 3 and 4 following transplantation, VEGF concentrations were increased in the experimental group compared with control group (P < 0.01). Moreover, VEGF concentrations were decreased over time in two groups (P < 0.01). Capillaries per unit area of the experimental group were significantly increased in infarct region compared with control group (P < 0.01). Results showed that after BMSCs transplantation, in myocardial infarct area, the concentration of VEGF is increased and promotes angiogenesis after myocardial infarction.

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