中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8186-8190.doi: 10.3969/j.issn.1673-8225.2010.44.005

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

袖套法建立大鼠肺移植模型的手术技巧

林江波1，陈道中2，康明强1，陈  椿1，林若柏1   

  1. 福建医科大学附属协和医院，1胸外科，2心外科，福建省福州市    350001
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 作者简介:林江波☆，男，1974年生，福建省泉州市人，汉族，2008年福建医科大学毕业，博士，副主任医师，主要从事同种异体肺移植、胸部微创外科研究。 linjiangbo.lin@yahoo.com.cn

Surgical skills for establishing a rat model of lung transplantation using cuff method

Lin Jiang-bo1, Chen Dao-zhong2, Kang Ming-qiang1, Chen Chun1, Lin Ruo-bai1   

  1. 1 Department of Thoracic Surgery, 2 Department of Cardiac Surgery, Union Hospital of Fujian Medical University, Fuzhou   350001, Fujian Province, China
  • Online:2010-10-29 Published:2010-10-29
  • About author:Lin Jiang-bo☆, Doctor, Associate chief physician, Department of Thoracic Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China linjiangbo.lin@yahoo.com.cn

PDF

729

被引次数

8

摘要：

背景：建立大鼠肺移植动物模型是研究肺移植后病理生理过程及其治疗的基础和关键，但因其需要精细外科技术，难度较大，限制了这一模型的应用。
目的：探讨袖套法建立稳定的大鼠肺移植模型的手术操作技巧。
方法：纳入60只大鼠，采用改良的三袖套技术建立大鼠左肺原位移植模型30例。供肺植入血管、支气管套接吻合的技巧：受体肺静脉采用90°角分布的“4针悬吊法”，使塌陷的静脉血管开口完全张开且方向固定，减少受体静脉干短拙或强行套入导致血管壁撕裂的可能。受体肺动脉采用120°角分布的“3针悬吊”兜开，套接时应注意供肺套管的方向。最后支气管采用内支架吻合。计算各阶段手术时间及移植成功率。
结果与结论：30例大鼠肺移植手术成功率为90%，移植后存活率100%，左供肺摘取需时(3±1) min，供肺完成体外套管时间分别为：肺动脉(2.0±0.5) min、肺静脉(3±1) min、支气管(2.0±0.5) min，供受体动静脉和支气管套管吻合时间分别为：肺动脉(4±1) min、肺静脉(8±3) min、支气管(2.0±0.5) min，总手术时间(55±10) min。提示改良的三袖套技术建立大鼠肺移植模型操作简便，手术成功率高，可为大鼠肺移植缺血再灌注损伤研究提供稳定可靠的动物模型。

关键词: 肺移植, 手术技巧, 动物模型, 大鼠, 方法改良

Abstract:

BACKGROUND: To establish a lung transplantation model in rats is the foundation and the key to pathophysiological process and treatment following lung transplantation; however, a precise surgical technique is required, as the difficulty is great to limit the application of the model.
OBJECTIVE: To investigate the surgical skills of establishing a stable rat lung transplantation model using cuff method.
METHODS: A total of 60 rats were included. Orthotopic left lung transplantation models were established in 30 rats by the modified three-cuff anastomotic technique. The techniques of pulmonary vascular and bronchial anastomosis are as follows: The recipient pulmonary vein was distracted by the“4-pin suspension”with the distribution of 90°angle，so that the collapsed vein was fully open and fixed, and reducing the possibility of tearing the vessel wall which was due to short recipient veins or forcible insection. The recipient pulmonary artery was distracted by the “3-pin suspension” with the distribution of 120° angle. However, the direction of donor lung casing should be pay more attention during anastomosis. Finally, the endo-stent was used for bronchial anastomosis. Meanwhile, the operation time and achievement ratio of transplantation at different stages were calculated.
RESULTS AND CONCLUSION: The achievement ratio of lung transplantation in 30 cases of rats was 90%; the survival rate of rats after lung transplantation was 100%. The time of left donor lung extraction was (3±1) minutes. The time of in vitro casing in donor lung was pulmonary artery (2±0.5) minutes, pulmonary vein (3±1) minutes, and bronchus (2±0.5) minutes, respectively. The time of arteriovenous and bronchus casing anastomosis was pulmonary artery (4±1) minutes, pulmonary vein (8±3) minutes, bronchus (2±0.5) minutes, respectively. The total operation time was (55±10) minutes. It is indicated that the modified three-cuff technique for establishing lung transplantation models in rats is simple with high achievement ratio of operation. It is a stable model for the study of ischemia-reperfusion injury in rats following lung transplantation.

中图分类号: