中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8183-8185.doi: 10.3969/j.issn.1673-8225.2010.44.004

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

改良三套管法建立大鼠左肺原位移植模型

范  凯，王建军，翟  伟，唐  建，万  黎   

  1. 华中科技大学附属协和医院胸外科，湖北省武汉市  430022
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 通讯作者: 翟伟，博士，副主任医师，副教授，华中科技大学附属协和医院胸外科，湖北省武汉市 430022
  • 作者简介:范凯★，男，1984年生，湖北省武汉市人，汉族，华中科技大学同济医学院附属协和医院在读硕士，主要从事肺移植和肺恶性肿瘤综合治疗的研究。 fankay0922@gmail.com
  • 基金资助:

    国家自然科学基金(30700767)。

Establishment of a rat orthotopic left lung transplantation model using modified three-cuff method

Fan Kai, Wang Jian-jun, Zhai Wei, Tang Jian, Wan Li   

  1. Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Zhai Wei, Doctor, Associate Chief physician, Associate professor, Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
  • About author:Fan Kai★, Studying for master’s degree, Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China fankay0922@gmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30700767*

PDF

490

被引次数

19

摘要：

背景：肺移植是治疗终末期肺部疾病的惟一方法。建立肺移植动物模型是肺移植基础研究的首要条件。
目的：建立一种简便、稳定和有效的同种大鼠异体左肺原位移植模型。
方法：50只雄性SD大鼠随机分为供体以及受体，采用改良三套管法进行大鼠左肺原位移植。首先完成供肺制备，并将供肺置于受体原左肺上方并保持供肺于4 ℃ LPD液环境中。按照左肺动脉-左肺静脉-左肺支气管的顺序依次完成供肺与受体相应结构的吻合。然后依次开放左肺静脉-肺动脉，调整潮气量，离断受体原左肺。观察手术成功率、手术时间，5周后对受体行血气分析。
结果与结论：手术成功率92%，手术时间为(55±3) min，术后存活均达5周。5周后阻断右肺门15 min前、后左肺静脉取血，血气分析差异无显著性意义(P > 0.05)。提示新改良的三套管法是一种简便稳定有效的大鼠原位左肺移植模型。

关键词: 原位肺移植, 大鼠, 动物模型, 套管, 同种异体

Abstract:

BACKGROUND: Lung transplantation is the sole cure for end-stage lung disease. The basic research addressing lung transplantation is based on animal models.
OBJECTIVE: To establish a new simple, stable, and available experimental model of rat lung orthotopic allograft transplantation.
METHODS: Fifty male rats were randomly grouped into donors and recipients; lung transplantation was performed with using new three-cuff technique. The donor lung was harvested, placed over left lung of recipients and maintained in 4℃ LPD solution. The donor lung was anastomosed to the recipient following the sequence from the left pulmonary artery, vein to the bronchus. Then the left pulmonary vein-artery was opened successively, and the left lung of recipient was removed. Blood gas of recipients was tested after 5 weeks.
RESULTS AND CONCLUSION: The successful rate of surgery was 92%, and the mean time for all the proceedings was (55±3) minutes. All the lung grafts were survivable over 5 weeks. Blood was collected from the left vein at 15 minutes prior to and after right pulmonary hilum occlusion, and the blood gas analysis showed there was no significant difference of the blood gas before and after occlusion (P > 0.05). The findings suggested that, new cuff method is a simple, reproducible and valid method for establishing models of rat orthotopic left lung transplantation.

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