中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7497-7500.doi: 10.3969/j.issn.1673-8225.2010.40.019

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

转化生长因子β3诱导骨髓间充质干细胞分化为软骨细胞

赵基栋，苗宗宁，钱寒光，彭  伟   

  1. 无锡第三人民医院修复重建外科，江苏省无锡市 214041
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 作者简介:赵基栋，男，1961年生，浙江省宁波市人，汉族，1983年南京医科大学毕业，副教授，主任，副主任医师，主要从事骨组织工程方面的研究。 zid66@hotmail.com

Differentiation of bone marrow mesenchymal stem cells into chondrocyte lineage induced by transforming growth factor beta 3

Zhao Ji-dong, Miao Zong-ning, Qian Han-guang, Peng Wei   

  1. Department of Cineplastic Surgery, Wuxi Third People’s Hospital, Wuxi  214041, Jiangsu Province, China
  • Online:2010-10-01 Published:2010-10-01
  • About author:Zhao Ji-dong, Associate professor, Associate chief physician, Department of Cineplastic Surgery, Wuxi Third People’s Hospital, Wuxi 214041, Jiangsu Province, China zid66@hotmail.com

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摘要：

背景：转化生长因子β是一族多肽类生长因子，胚胎形成期可诱导原始的间充质干细胞分化形成软骨组织，有研究报道转化生长因子β3和碱性成纤维细胞生长因子可促进软骨细胞的代谢和增殖。
目的：验证转化生长因子β3体外诱导骨髓间充质干细胞分化为软骨细胞的可行性。
方法：骨髓来源于髋关节手术时的松质骨碎片或于下肢骨开放性手术时收集，分离培养骨髓间充质干细胞，鉴定其表面抗原，用含体积分数10%胎牛血清以及10 μg/L转化生长因子β3的条件培养基诱导，诱导后细胞通过软骨细胞特征性染色，即甲苯氨蓝染色以及Ⅱ型胶原免疫组织化学染色鉴定。
结果与结论：骨髓间充质干细胞表面抗原CD73，CD90，CD105阳性，CD14，CD34，CD45，CD106以及HLA-DR阴性。诱导后细胞形态明显改变，甲苯氨蓝以及Ⅱ型胶原染色结果阳性。提示骨髓间充质干细胞在转化生长因子β3作用下，体外可分化为软骨细胞，可以作为组织工程种子细胞的一种有效来源。

关键词: 转化生长因子, 骨髓间充质干细胞, 软骨细胞, 体外, 诱导

Abstract:

BACKGROUND: Transforming growth factor β，a polypeptide growth factor, can induce original mesenchymal stem cells (MSCs) differentiation into cartilage tissue during embryogenesis phase. Previous studies have reported that transforming growth factor β3 and basic fibroblast growth factor can contribute to metabolism and proliferation of chondrocytes.
OBJECTIVE: To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocyte lineage by transforming growth factor β3 in vitro.
METHODS: Bone marrow was collected from cancellous bone chips during hip joint surgery or during lower limb bones open operation to isolate and culture BMSCs. Its surface antigen was identified. Conditioned medium containing 10% fetal bovine serum and 10 μg/L transforming growth factor β3 was used. Following induction, cells were stained using toluidine blue staining and type II collagen immunohistochemistry.
RESULTS AND CONCLUSION: BMSCs were positive for CD73, CD90, CD105, but negative for CD14, CD34, CD45, CD106 and HLA-DR. The morphology of induced cells was obviously changed. The results of toluidine blue staining and type II collagen staining were positive. These indicated that transforming growth factor β3 has the ability to promote BMSCs differentiation into chondrocytes. BMSCs may prove to be a useful source of tissue engineered seed cells.

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