中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (38): 7069-7074.doi: 10.3969/j.issn.1673-8225.2010.38.010

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

羧甲基壳聚糖-羧甲基纤维素防粘连膜的制备及其理化特性

肖海军1，侯春林2，薛  锋1   

  1. 1上海市奉贤区中心医院骨科，上海市  201400；2解放军第二军医大学长征医院骨科，上海市  200003
  • 出版日期:2010-09-17 发布日期:2010-09-17
  • 通讯作者: 侯春林，主任医师，教授，博士生导师，解放军第二军医大学长征医院骨科，上海市 200003 chunlin_hou@yahoo.com
  • 作者简介:肖海军☆，男，1971年生，四川省中江县人，2006年解放军第二军医大学毕业，博士，副主任医师，副教授，主要从事创伤骨科及修复重建外科研究。xiaohaijun89@163.com
  • 基金资助:

    国家自然科学基金资助项目(30170956)。

Preparation and physicochemical properties of carboxymethyl chitosan-carboxymethyl cellulose anti-adhesive film

Xiao Hai-jun1, Hou Chun-lin2, Xue Feng1   

  1. 1 Department of Orthopedics, Fengxian District Center Hospital, Shanghai   201400, China; 2 Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai   200003, China
  • Online:2010-09-17 Published:2010-09-17
  • Contact: Hou Chun-lin, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China chunlin_hou@yahoo.com
  • About author:Xiao Hai-jun☆, Doctor, Associate chief physician, Associate professor, Department of Orthopedics, Fengxian District Center Hospital, Shanghai 201400, China xiaohaijun89@163.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30170956*

PDF

625

被引次数

27

摘要：

背景：壳聚糖能有效预防粘连，可制成不同的剂型，如凝胶、溶液、海绵状以及薄膜等，但其缺点是溶液、凝胶易流动，不易在局部形成较高浓度，而单纯应用壳聚糖制成海绵状及薄膜机械强度及韧性不够。
目的：以羧甲基壳聚糖、羧甲基纤维素为主要成分，制备具有优良物理、生物性能，预防粘连的生物膜。
方法：将羧甲基壳聚糖和羧甲基纤维素按一定比例混合，加入戊二醛、硫酸铝铵进行双交联，甘油增塑。根据膜的色泽、拉伸强度、吸水率、溶胀比等指标通过正交试验筛选处方构成，优化膜的制备工艺，初步确定膜的制备工艺流程。通过扫描电镜、红外光谱等对制备样本的理化特性进行检测，并将膜植入SD大鼠体内观察膜的降解情况。
结果与结论：得到优化后膜的处方构成为：羧甲基壳聚糖/羧甲基纤维素为1∶1；硫酸铝铵浓度为0.15%；甘油含量为0.8%；戊二醛含量为0.003%。在以上工艺条件下制得的膜呈半透明状，微黄，上表面略粗躁，下表面光滑；膜的平均厚度为 0.09 mm；吸水率为964%；溶胀比为3.25；干态下膜的最大拉伸强度为20 MPa，湿态下膜的最大拉伸强度为5 MPa；接触角平均为35°。羧甲基壳聚糖和羧甲基纤维素间形成了较强的分子间作用力。膜表面结构呈相互交错的纤维状，表面有不规则的孔状结构。膜植入鼠体内后10 d左右水化成凝胶，1个月后体内完全降解吸收。提示羧甲基壳聚糖-羧甲基纤维素膜是一种生物相容性良好、可降解吸收、具有一定手术缝合强度的生物膜。

关键词: 羧甲基壳聚糖, 羧甲基壳聚糖-羧甲基纤维素, 膜, 粘连, 生物材料

Abstract:

BACKGROUND: Chitosan can prevent adhesion, and be made into different forms, such as gel, solution, sponge-like and thin films, etc., but the solution and the gel flow easily, and it is difficult to form a high local concentration, while simple chitosan-made sponge and thin films have insufficient mechanical strength and toughness.
OBJECTIVE: To prepare a kind of carboxymethyl chitosan (CMCH)-carboxymethyl cellulose (CMC) crosslinked film with fine physical and biologic properties for postoperative anti-adhesion.
METHODS: CMCH-CMC film were prepared successfully at different ratios of CMCH and CMC, glutaraldehyde and ammonium aluminium sulfate cross-linked, followed by glycerin plasticity. The formula was chosen through orthogonal experimental design according to film color, tensile strength, breaking elongation, swelling ratio, water absorption ratio, touching angle. The preparation techniques were optimized and the preparation procedure was determined. The physiochemical characteristics of the film sample were detected through scanning electron microscopy and infrared spectrum. The biodegradable time of the film in SD rats was observed.
RESULTS AND CONCLUSION: The optimized formula was as follows: the ratio of CMCH to CMC was 1: 1; the concentration of ammonium aluminium sulfate, glycerin, and glutaraldehyde was 0.15%, 0.8%, 0.003 0% respectively. The obtained film was yellowish, translucent, at the thickness of 0.09 mm. The water absorption ratio of the prepared film was 964%, the swelling ratio was 3.25; the maximal tensile strength was 20 MPa in dry state while 5 MPa in wet state; the touching angle was averagely 35°. There are some strong interactions and good compatibility between CMCH and CMC molecules in the cross-linked film. The morphology of CMCH-CMC film had crossed fibroid structure with irregular porous structures at the surface. The CMCH-CMC film placed in rats hydrated to a gel within 10 days and absorbed completely at the site 1 month later. The CMCH-CMC film has good biocompatibility, biodegradability, and feasible strength that could be sutured.

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