中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6931-6934.doi: 10.3969/j.issn.1673-8225.2010.37.021

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

佐剂性关节炎模型关节液与血清白细胞介素17及肿瘤坏死因子α表达及青蒿琥酯的干预　

莫汉有，石宇红，杨  敏，王丽芳   

  1. 桂林医学院附院风湿免疫科，广西壮族自治区桂林市  541000　
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 石宇红，硕士，副教授，桂林医学院附院风湿免疫科，广西壮族自治区桂林市 541000 stone1977tree@yahoo.com
  • 作者简介:莫汉有★，男，1973年生，广西壮族自治区贵港人，汉族，1995年广西医科大学毕业，硕士，副教授，硕士生导师，主要从事风湿病方面的研究。 mohanyou@sohu.com
  • 基金资助:

    广西卫生厅课题(Z2006204)，课题名称“内皮抑素与佐剂性关节炎的相关性研究”；广西卫生厅课题(Z2009044)，课题名称“MTHFR基因C677T多态性与甲氨蝶呤抗风湿治疗血液不良反应的相关性研究”；广西桂林市课题(20090538)，课题名称“MTHFR基因C677T多态性与甲氨蝶呤抗风湿治疗血液不良反应的相关性研究”。

Influence of artesunate on synovial fluid and serum expressions of interleukin-17 and tumor necrosis factor-alpha in rabbits with adjuvant arthritis

Mo Han-you, Shi Yu-hong, Yang Min, Wang Li-fang   

  1. Department of Rheumatology, Affiliated Hospital of Guilin Medical University, Guilin  541000, Guangxi Zhuang Autonomous Region, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Shi Yu-hong, Master, Associate professor, Department of Rheumatology, Affiliated Hospital of Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China stone1977tree@yahoo.com
  • About author:Mo Han-you★, Master, Associate professor, Master’s supervisor, Department of Rheumatology, Affiliated Hospital of Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China mohanyou@sohu.com
  • Supported by:

    the Health Department Project of Guangxi, No. Z2006204*, Z2009044*; Guilin Project, No. 20090538*

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摘要：

背景：已证实青蒿琥酯具有抗风湿作用，但其具体作用机制尚不明确。
目的：探讨青蒿琥酯对佐剂性关节炎模型兔关节液及血清中白细胞介素17及肿瘤坏死因子α表达的影响。
方法：建立佐剂性关节炎实验兔模型，造模诱发第1天，青蒿琥酯组按20 mg/(kg•d)灌服青蒿琥酯7 d，羟氯喹组按5 mg/(kg•d)灌服羟氯喹7 d，模型对照组灌服生理盐水3 mL/d。另取10只未经干预的大耳白兔作为正常对照组。分别于造模结束时及给药5周后，应用酶联免疫吸附法检测各组关节液及血清中白细胞介素17及肿瘤坏死因子α的水平。
结果与结论：青蒿琥酯组、羟氯喹组关节炎症状得到明显改善，关节液及血清中的白细胞介素17、肿瘤坏死因子α质量浓度均明显降低(P < 0.05或P < 0.01)，且青蒿琥酯组降低幅度明显大于羟氯喹组(P < 0.05)。提示青蒿琥酯可能通过抑制白细胞介素17及肿瘤坏死因子α的分泌而产生免疫调节作用，其抗炎疗效优于羟氯喹。

关键词: 佐剂性关节炎, 关节液, 血清, 白细胞介素17, 肿瘤坏死因子&alpha, 青蒿琥酯

Abstract:

BACKGROUND: It has been proved that rheumatoid can be cured by artesunate. But the specific mechanism remains poorly understood. 
OBJECTIVE: To explore the effects of artesunate on the level of interleukin-17 and tumor necrosis factor-α (TNF-α) in rabbit with adjuvant arthritis.
METHODS: The rabbits were prepared for adjuvant arthritis models. From the first day after model preparation, rabbits in the artesunate group were drenched with artesunate (20 mg/kg per day) for 7 days. Hydroxychlorquine group were infused with hydroxychlorquine (5 mg/kg per day) for 7 days. Model control group was infused with normal saline (3 mL/kg per day) for 7 days. Additional 10 normal rabbits served as controls. The enzyme-linked immunosorbent assay (ELISA) was used to detect synovial fluid and serum level of IL-17 and TNF-α at the end of model preparation and 5 weeks after medication.
RESULTS AND CONCLUSION: Compared with model rats, the serum and synovial fluid levels of IL-17 and TNF-α in the artesunate group and hydroxychlorquine group were obviously decreased (P < 0.05 or P < 0.01), especially in the Artesunate group, which was greater than that of the hydroxychlorquine group (P < 0.05). The results revealed that artesunate can down-regulate the serum and synovial fluid level of IL-17 and TNF-α of adjuvant arthritis. Artesunate has better effect than hydroxychlorquine on treatment of inflammation.

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