中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (20): 3705-3708.doi: 10.3969/j.issn.1673-8225.2010.20.022

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

反流性食管炎大鼠模型的制备：不同管径内支架支撑幽门及前胃结扎

郑朝旭1，林伟斌2，余俊峰1，陈流华1   

  1. 1中山大学附属第一医院微创外科，广东省广州市   510080；
    2广州市番禺区何贤纪念医院普外科，广东省广州市511400
  • 出版日期:2010-05-14 发布日期:2010-05-14
  • 作者简介:郑朝旭，男，1970年生，福建省泉州市人，汉族，2001年中山医科大学毕业，博士，副主任医师，硕士生导师，主要从事胃肠道、肝胆胰脾疾病的微创治疗研究。 zhengchaoxu@yahoo.com
  • 基金资助:

    广东省科技计划项目资助（2005 B31201015）。

Preparation of reflux esophagitis rat models: Partial pylorus ligation plus forestomach ligation with different diameters of stents

Zheng Chao-xu1, Lin Wei-bin2, Yu Jun-feng1, Chen Liu-hua1   

  1. 1Department of Minimally Invasive Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China;   
    2Department of General Surgery, Hexian Memorial Hospital, Panyu District, Guangzhou 511400, Guangdong Province, China
  • Online:2010-05-14 Published:2010-05-14
  • About author:Zheng Chao-xu, Doctor, Associate chief physician, Master’s supervisor, Department of Minimally Invasive Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China zhengchaoxu@yahoo.com
  • Supported by:

    the Science and Technology Planning Project of Guangdong Province, No. 2005 B31201015*

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摘要：

背景：部分幽门结扎法是制备反流性食管炎动物模型的常用方法之一。幽门结扎口径大小关系到能否有效建立反流性食管炎模型。

目的：比较运用不同管径内支架并部分幽门加前胃结扎制备大鼠反流性食管炎模型的差异，以寻求合理的制备模型方法。

方法：将40只雌性Wistar大鼠随机分为2组。分别采用外径2.9 mm内支架和外径3.7 mm内支架支撑结扎幽门，并加前胃结扎减少胃容量建立动物模型。观察大鼠体质量及7 d存活率。7 d后处死大鼠，取食管下段组织标本观察食管炎发生率。

结果与结论：2.9 mm内支架组和3.7 mm内支架组7 d存活率分别为35%(7/20)和68%(13/19)，差异有显著性意义(P=0.038)；两组食管炎发生率分别为86%(6/7)和77%(10/13)，以及两组存活大鼠结扎后每天体质量差异均无显著性意义(P > 0.05)。结果表明，采用部分幽门加前胃结扎可成功建立反流性食管炎大鼠模型，幽门结扎后管径与大鼠存活率密切相关。

关键词: 反流性食管炎, 动物模型, 幽门结扎法, 大鼠, 管径

Abstract:

BACKGROUND: Partial pylorus ligation is a common method for establishment of reflux esophagitis (RE) model. The diameter of pylorus ligation is closely related to the efficacy of establishment.

OBJECTIVE: To investigate the diversity of partial pylorus ligation with different diameters of stents plus forestomach ligation and to find a rational method to induce RE in rats.

MATERIALS: Forty female Wistar rats were randomly divided into 2 groups, and 2.9 mm diameter and 3.7 mm diameter stents were inserted through the incision to sustain the pylorus and extracted after pylorus ligation. Then ligation of the transitional region between the forestomach and stomach was performed additionally to establish RE model. The body weights and 7-day survival rates of rats in each group were observed. The rats were sacrificed 7 days after the procedures, and the incidences of esophagitis of two groups were counted.

RESULTS AND CONCLUSION: The 7-day survival rates of 2.9 mm diameter and 3.7 mm diameter stents groups were 35% (7/20) and 68.4% (13/19), respectively (P = 0.038). The incidences of esophagitis were 85.7% (6/7) and 76.9% (10/13), respectively. There were no significant differences in the body weights of everyday postoperatively in survival rats between two groups (P > 0.05). Successful RE model in rats can be established by partial pylorus ligation plus forestomach ligation. The pylorus ligation diameter is closely related to the survival rates of rats.

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