中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (18): 3339-3342.doi: 10.3969/j.issn.1673-8225.2010.18.026

• 移植与中医药 transplantation and traditional Chinese medicine • 上一篇    下一篇

大鼠背部超长原位回植皮瓣成活：人参皂甙Rb1影响的验证

曹  畅，李  棋，岑  瑛   

  1. 四川大学华西医院烧伤整形科，四川省成都市 610041
  • 出版日期:2010-04-30 发布日期:2010-04-30
  • 通讯作者: 岑 瑛，教授，四川大学华西医院烧伤整形科，四川省成都市 610041
  • 作者简介:曹 畅★，女，1982年生，四川省绵阳市人，汉族，四川大学烧伤整形在读硕士，医师，主要从事创面修复及瘢痕防治研究。 viviennecao@yahoo.cn

Effect of ginsenoside rb1 on survival of dorsal random-pattern skin flap with large length-to-width ratio in rats

Cao Chang, Li Qi, Cen Ying   

  1. Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu   610041, Sichuan Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Cen Ying, Professor, Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Cao Chang★, Studying for master’s degree, Physician, Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China viviennecao@yahoo.cn

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摘要：

背景：国内外将人参皂甙Rb1应用于心、脑、肺、肾及肝损害的保护与治疗的报道较多，但是将人参皂甙Rb1用于皮瓣的研究还未见报道。
目的：观察腹腔注射人参皂甙Rb1对大鼠背部超长宽比例随意皮瓣成活的影响。
方法：将健康成年SD大鼠以数字表法随机分为实验组及对照组。于大鼠背部设计制备蒂部在尾部的超长皮瓣80 mm× 20 mm(长宽比4∶1)，原位回植。术中即刻、术后第1~5天腹腔注射2 mg/kg人参皂甙Rb1生理盐水溶液，对照组腹腔注射等量生理盐水。术后1 d取材测定皮瓣一氧化氮和丙二醛含量，术后10 d测定皮瓣存活率，取皮瓣近、中、远段行苏木精-伊红染色观察。
结果与结论：术后1 d实验组皮瓣一氧化氮含量高于对照组(P < 0.01)，丙二醛含量低于对照组(P < 0.01)。术后10 d，实验组皮瓣成活率明显高于对照组(P < 0.001)。组织学观察显示，实验组水肿及炎性细胞浸润较对照组明显减轻，纤维增生较对照组明显。结果提示围手术期腹腔注射人参皂甙Rb1能明显改善大鼠皮瓣血供，提高皮瓣的成活面积和成活率，其作用机制可能与促进内皮细胞分泌释放NO、清除氧自由基、降低脂质过氧化产物、促进皮瓣血管新生有关。

关键词: 人参皂甙Rb1, 超长皮瓣, 血管新生, 一氧化氮, 丙二醛

Abstract:

BACKGROUND: Ginsenoside Rb1 has been extensively used in the protection and treatment of heart, encephalon, lung, kidney and liver damage. However, its application in skin flap is rare.
OBJECTIVE: To investigate the effects of intraperitoneal injection of ginsenoside Rb1 on the survival of the dorsal random-pattern skin flap with large length-to-width ratio in rats.
METHODS: Healthy adult SD rats were randomly divided into experimental and control groups. A caudally based dorsal random pattern skin flap, 80 mm×20 mm (length: width = 4: 1), was symmetrically made. Ginsenoside Rb1 (2 mg/kg) was intraperitoneally injected into the experimental group rats, and the same volume of normal saline was injected into the control group. Malonyl dialdehyde (MDA) and nitric oxide (NO) level of the flaps were tested 1 day after operation; the amount of viable tissues of the flaps were examined by planimetry 10 days after operation. Specimens from the proximal, middle and distal flaps were harvested for HE staining to examine the microstructure. 
RESULTS AND CONCLUSION: At the first day after operation, NO level was higher in the experimental group than the control group (P < 0.01), while MDA level was lower than the control group (P < 0.01). At the 10th day after operation, the survival rate of the flap was significantly greater in the experimental group than the control group (P < 0.001). Histological observation showed that compared with the control group, the edema and inflammatory cells infiltration were less, while the fiber hyperplasia and the microvascular growth were more obvious in the experimental group. Results show that intraperitoneal injection of ginsenoside Rb1 can enhance the blood supply of the flaps and improve the survival of the random-pattern skin flaps with large length-to-width ratio in rats. This may involve its effects of improving NO activity, decreasing lipid peroxidation, and promoting angiogenesis of skin flaps.

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