中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (6): 1043-1047.doi: 10.3969/j.issn.1673-8225.2010.06.019

• 干细胞移植 • 上一篇    下一篇

自体骨髓间充质干细胞心肌移植后的分化及存活

蔡红雁，聂  俊，陈丽星，赵  玲，郭  涛，肖践明   

  1. 昆明医学院第一附属医院心内科，云南省昆明市  650031
  • 出版日期:2010-02-05 发布日期:2010-02-05
  • 通讯作者: 肖践明，教授，昆明医学院第一附属医院心内科，云南省昆明市 650031 Jianmingxiao@163.com
  • 作者简介:蔡红雁，女，1971年生，汉族，云南省昆明市人，汉族，1990年昆明医学院毕业，博士，主治医师，主要从事冠心病基础及介入的研究。 hyflykm@sina.com
  • 基金资助:

    云南省教育厅基金资助项目(5Z0470C)。

Differentiation and survival of autologous bone marrow mesenchymal stem cells following transplantation into the myocardium

Cai Hong-yan, Nie Jun, Chen Li-xing, Zhao Ling, Guo Tao, Xiao Jian-ming   

  1. Department of Cardiology, First Affliated Hospital of Kunming Medical College, Kunming   650031, Yunnan Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Xiao Jian-ming, Professor, Department of Cardiology, First Affliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China Jianmingxiao@163.com
  • About author:Cai Hong-yan☆, Doctor, Attending physician, Department of Cardiology, First Affliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China hyflykm@sina.com
  • Supported by:

    the Foundation of Education Department of Yunnan Province, No. 5Z0470C*

PDF

454

被引次数

7

摘要：

背景：骨髓间充质干细胞移植入急性心肌梗死组织后是否能迁移、分化及存活，目前尚不清楚。

目的：观察兔骨髓间充质干细胞移植入急性心肌梗死组织后，在体内的迁移、分化及其存活。

方法：分离培养兔骨髓间充质干细胞，体外DAPI标记细胞。结扎兔左前降支制作急性心肌梗死模型，造模成功后30只新西兰大耳兔抽签法分为骨髓间充质干细胞组和对照组，每组15只。骨髓间充质干细胞组于冠状动脉结扎后1 h在梗死周边区用微量注射器分4点注射自体骨髓间充质干细胞。对照组于相同部位注射等量生理盐水。每点注射体积30 μL。分别于移植后10 min，3 d，4周时各组各处死5只动物。取心脏行冰冻切片，荧光显微镜观察DAPI标记骨髓间充质干细胞的分布情况；免疫荧光法检测标记细胞是否表达心肌特异性肌钙蛋白Ⅰ和α-肌动蛋白。

结果与结论：DAPI标记的骨髓间充质干细胞在兔急性心肌梗死模型心脏微环境中，蓝色细胞核分布广泛，呈椭圆形，似心肌细胞核，其排列方向与宿主心肌纤维方向一致，间接免疫荧光法检测胞浆心肌特异性蛋白肌钙蛋白Ⅰ、α-肌动蛋白均为阳性。说明缺血心肌内移植入骨髓间充质干细胞，可在局部微环境的刺激下向心肌细胞定向分化。

关键词: 心肌梗死, 骨髓间充质干细胞, 细胞移植, 肌钙蛋白Ⅰ, &alpha, -肌动蛋白

Abstract:

BACKGROUND: It was uncertain that the migration, differentiation and survival of transplanted bone marrow mesenchymal stem cells (BMSCs) into myocardium after the acute myocardial infarction.

OBJECTIVE: To investigate the migration, differentiation and survival of rabbit transplanted autologous BMSCs in myocardium after the acute myocardial infarction.

METHODS: Rabbit BMSCs were isolated and labeled by DAPI in vitro. Rabbit left anterior descending branch was ligated to establish acute myocardial infarction models. Following successful model establishment, 30 New Zealand rabbits were assigned to BMSC and control groups (n = 15). In the BMSC group, autologous BMSCs were infused into the surrounding sites of the infracted region by 4 points 1 hour following coronary artery ligation. In the control group, the same region was injected with an equal volume of saline. Injection volume was 30 μL in each point. Five animals from each group were sacrificed 10 minutes, 3 days and 4 weeks following transplantation. The heart was obtained to undergo frozen sections. The distribution of DAPI-labeled BMSCs was observed using fluorescence microscope. Immunofluorescence method was used to examine the troponin Ⅰ and α-actin.

RESULTS AND CONCLUSION: DAPI-labeled BMSCs with blue nuclei were distributed extensively in the myocardium of the cell transplantation group, ovoid in shape and arranged in parallel with the cardiac muscle fibers. Troponin Ⅰ and α-actin were positive immunofluorescently in the cytoplasm of the labeled BMSCs. Results indicated that transplanted BMSCs in the ischemic myocardium could differentiate into myocardial cells under stimulation of local microenvironment.

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