中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (30): 7869-7879.doi: 10.12307/2026.961

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

基于牙本质基质蛋白1构建纳米仿牙本质陶瓷根管封闭剂材料及表观性能

韩友军1，2，罗超凡1，2，李一凡1，2，邵子瑜1，2，胡  杨1，2   

  1. 1新疆医科大学第一附属医院(附属口腔医院)口腔修复种植科，新疆维吾尔自治区乌鲁木齐市   830054；2新疆维吾尔自治区口腔医学研究所，新疆维吾尔自治区乌鲁木齐市   830054
  • 收稿日期:2026-05-28 修回日期:2026-06-08 出版日期:2026-10-28 发布日期:2026-09-11
  • 通讯作者: 胡杨，硕士，主任医师，副教授，新疆医科大学第一附属医院(附属口腔医院)口腔修复种植科，新疆维吾尔自治区乌鲁木齐市 830054；新疆维吾尔自治区口腔医学研究所，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:韩友军，男，1998年生，贵州省遵义市人，在读硕士研究生，住院医师，主要从事口腔修复学方面的研究。
  • 基金资助:
    新疆维吾尔自治区重点研发任务专项(2016B03049-2)，项目负责人：胡杨；上海宝山区卫生健康委员会科研课题计划任务书[BSZK-2023-BP02(01)]，项目参与人：胡杨

Construction and characterization of a dentin matrix protein-1-based nanobionic dentin ceramic root canal sealer

Han Youjun1, 2, Luo Chaofan1, 2, Li Yifan1, 2, Shao Ziyu1, 2, Hu Yang1, 2   

  1. 1Department of Prosthodontics and Implantology, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 2Xinjiang Uygur Autonomous Region Institute of Stomatology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2026-05-28 Revised:2026-06-08 Online:2026-10-28 Published:2026-09-11
  • Contact: Hu Yang, MS, Chief physician, Associate professor, Department of Prosthodontics and Implantology, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Xinjiang Uygur Autonomous Region Institute of Stomatology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Han Youjun, MS candidate, Physician, Department of Prosthodontics and Implantology, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Xinjiang Uygur Autonomous Region Institute of Stomatology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:
    Xinjiang Uygur Autonomous Region Key Research & Development Task Special Project, No. 2016B03049-2 (to HY); Shanghai Baoshan District Health Commission Scientific Research Project Plan Task Book, No. BSZK-2023-BP02(01) (to HY [project participant])

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摘要：



文题释义：
牙本质基质蛋白1：是一种酸性非胶原基质蛋白，在牙本质和骨组织中高度表达，也是牙本质细胞的特异性蛋白，对牙本质和骨的形成及矿化具有重要作用。
仿牙本质陶瓷：是一类通过纳米尺度材料设计与制备方法，模拟天然牙本质化学成分、微观结构和力学性能的陶瓷类生物材料。

背景：现阶段，多数根管封闭剂仍存在生物相容性欠佳、机械力学性能不足以及降解性不理想等问题，纳米β-磷酸三钙、纳米羟基磷灰石与牙体硬组织无机成分类似，且具备优异的生物相容性和降解性，在根管封闭剂临床应用领域展现出重要的研究价值。
目的：分别以牙本质基质蛋白1、谷氨酸、甘氨酸为软模板调控纳米羟基磷灰石、纳米β-磷酸三钙的结晶与成核并进行性能表征研究，旨在为构建新型根管封闭剂前体材料提供一定的理论和实验依据。 
方法：分别称取不同质量的纳米羟基磷灰石、纳米β-磷酸三钙加入不同浓度的牙本质基质蛋白1、L-谷氨酸、甘氨酸溶液中，调节pH值至10.0并于37.0 ℃水浴持续振荡24 h后冷却至室温，离心后洗涤沉淀物，然后将各组样品在-50 ℃下冻干24 h，备用。采用扫描电子显微镜、X射线能量色散谱仪、拉曼光谱仪、X射线衍射仪、X射线光电子能谱技术等方法对各组材料的形貌及结构进行表征与分析。
结果与结论：①扫描电子显微镜观察显示牙本质基质蛋白1、谷氨酸与甘氨酸对纳米羟基磷灰石和纳米β-磷酸三钙颗粒的排列及聚集态有明显影响，可呈现出不同特征；②X射线能量色散谱仪检测显示各组样品中含有均匀分布的O、Ca、P元素，纳米羟基磷灰石及纳米β-磷酸三钙各组样品的Ca/P均较接近羟基磷灰石的理想值及β-磷酸三钙的理论值；③拉曼光谱仪、X射线衍射仪、X射线光电子能谱仪检测显示各组复合材料未出现新的特征峰和元素，但出现部分化学态变化以及特征峰强度增强特征；④以牙本质基质蛋白1、谷氨酸、甘氨酸为软模板对纳米羟基磷灰石、纳米β-磷酸三钙有一定的调控作用，且调控后的材料表征性能稳定。牙本质基质蛋白1/纳米羟基磷灰石与牙本质基质蛋白1/纳米β-磷酸三钙表征性能相对较优，为构建新型根管封闭剂奠定了实验基础和理论依据。
https://orcid.org/0009-0009-6061-8295 (韩友军) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 根管封闭剂, 软模板, 牙本质基质蛋白1, 谷氨酸, 甘氨酸, 生物陶瓷, 纳米羟基磷灰石, 纳米β-磷酸三钙, 根管治疗

Abstract: BACKGROUND: Currently, most root canal sealers still suffer from issues such as poor biocompatibility, insufficient mechanical and mechanical properties, and suboptimal degradation. Nano-β-tricalcium phosphate and nano-hydroxyapatite are similar to the inorganic components of dental hard tissues and possess excellent biocompatibility and degradation properties, demonstrating significant research value in the clinical application of root canal sealers.
OBJECTIVE: To soft templates to regulate the crystallization and nucleation of nano-hydroxyapatite and nano-β-tricalcium phosphate with Dentin matrix protein 1, glutamic acid and glycine as soft templates, and to characterize the performance, aiming to provide a theoretical and experimental basis for the construction of new root canal sealer precursor materials.
METHODS: Different masses of nano-hydroxyapatite and nano-β-tricalcium phosphate were weighed and added to solutions of different concentrations of dentin matrix protein-1, L-glutamic acid, and glycine. The pH was adjusted to 10.0, and the mixture was heated in a 37.0°C water bath while continuously shaking for 24 hours, then cooled to room temperature. After centrifugation, the precipitates were washed, and the samples from each group were lyophilized at -50°C for 24 hours for later use. The morphology and structure of the materials in each group were characterized and analyzed using methods such as scanning electron microscopy, X-ray energy dispersive spectroscopy, Raman spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy.  
RESULTS AND CONCLUSION: (1) Scanning electron microscopy results showed that dentin matrix protein-1, glutamic acid, and glycine had a significant impact on the arrangement and aggregation state of nano-hydroxyapatite and nano-β-tricalcium phosphate particles, presenting different characteristics. (2) X-ray energy dispersive spectroscopy results showed that the samples in each group contained uniformly distributed O, Ca, and P elements, with the Ca/P ratios in the nano-hydroxyapatite and nano-β-tricalcium phosphate groups being relatively close to the ideal value of hydroxyapatite and the theoretical value of β-tricalcium phosphate. (3) Raman, X-ray diffraction, and X-ray photoelectron spectroscopy results showed that no new characteristic peaks or elements were observed in the composite materials of each group, but some changes in chemical states and enhanced intensity of characteristic peaks were observed. (4) Using dentin matrix protein-1, glutamic acid, and glycine as soft templates had a certain regulatory effect on nano-hydroxyapatite and nano-β-tricalcium phosphate, and the performance of the regulated materials was stable. Therefore, the characterization performance of dentin matrix protein-1/nano-hydroxyapatite and dentin matrix protein-1/nano-β-tricalcium phosphate is relatively superior, laying an experimental foundation and theoretical basis for constructing novel root canal sealers. 


Key words: root canal sealer, soft template, dentin matrix protein-1, glutamic acid, glycine, bioceramic, nano-hydroxyapatite, nano-β-tricalcium phosphate, root canal treatment

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