中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (35): 5702-5708.doi: 10.12307/2024.592
• 组织构建综述 tissue construction review • 上一篇 下一篇
李昱林1,俞海鹏1,唐华菁1,张梓桐2,林兴南1
收稿日期:
2023-11-15
接受日期:
2023-12-20
出版日期:
2024-12-18
发布日期:
2024-03-15
通讯作者:
林兴南,口腔医学博士,硕士生导师,副主任医师,副教授,浙江中医药大学口腔医学院,浙江省杭州市 310000
作者简介:
李昱林,男,1998年生,湖南省邵阳市人,汉族,浙江中医药大学口腔医学在读硕士,住院医师,主要从事骨组织工程和口腔正畸学研究。
基金资助:
Li Yulin1, Yu Haipeng1, Tang Huajing1, Zhang Zitong2, Lin Xingnan1
Received:
2023-11-15
Accepted:
2023-12-20
Online:
2024-12-18
Published:
2024-03-15
Contact:
Lin Xingnan, MD, Master’s supervisor, Associate chief physician, Associate professor, School of Stomatology, Zhejiang Chinese Medical University, Hangzhou 310000, Zhejiang Province, China
About author:
Li Yulin, Master candidate, Physician, School of Stomatology, Zhejiang Chinese Medical University, Hangzhou 310000, Zhejiang Province, China
Supported by:
摘要:
文题释义:
小檗碱:是一种具有骨诱导作用的天然异喹啉类化合物,具有替代骨组织工程中生长因子的潜力。
背景:小檗碱具有诱导各种间充质干细胞在正常状态和高糖、感染及炎症等特殊状态下成骨分化的潜力,可替代生长因子诱导种子细胞成骨,是一种具有骨诱导潜力的天然小分子药物,在骨组织工程领域有着巨大的应用前景。
目的:回顾和总结小檗碱成骨机制及效能,尤其是在高糖、感染及炎症环境下的成骨潜力,以及小檗碱生物安全性方面的研究进展,为其在骨组织工程中的发展应用提供理论依据。结果与结论:①小檗碱能用于治疗包括骨病在内的多种疾病,其具有促进骨再生作用,文章系统综述了小檗碱对骨再生作用机制及体内外研究。②研究表明小檗碱能够通过促骨生成、抑制破骨细胞形成及其活性、抗骨质疏松等方面发挥骨修复作用,主要通过参与Wnt/β-catenin,PI3K/AKT,EGFR/MEK/p38MAPK,cAMP/PKA/CREB和ERK等信号通路,表现出优异的成骨诱导分化潜力。③小檗碱还可以解除因高糖、感染及炎症引起的细胞成骨分化抑制,这为治疗患有糖尿病的骨缺损患者或骨缺损部位有感染及炎症的患者提供了更多可能。④小檗碱还具有毒性小、价格低廉和易于获取(目前已经能够人工合成)等优点,是较为理想的骨诱导潜力药物。⑤近年来应用小檗碱治疗骨缺损趋于局部使用,主要通过与骨组织工程技术结合改善生物利用度,在动物实验中表现出良好的骨修复作用和生物安全性,而且临床前实验表明,小檗碱在糖尿病、局部感染及炎症状态下仍然表现出优秀的骨再生潜力。⑥未来还需要更多的研究完整地揭示小檗碱的成骨机制和生物安全性等问题,并寻求最合适的控释载体制作出机械强度、疗效及生物安全性均佳的人工骨替代材料。
https://orcid.org/0009-0007-0566-5051(李昱林);https://orcid.org/0000-0002-9264-7236(林兴南)
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程
中图分类号:
李昱林, 俞海鹏, 唐华菁, 张梓桐, 林兴南. 小檗碱促进骨再生的机制、安全性及在骨组织工程中的应用[J]. 中国组织工程研究, 2024, 28(35): 5702-5708.
Li Yulin, Yu Haipeng, Tang Huajing, Zhang Zitong, Lin Xingnan. The mechanism, safety and application of berberine in promoting bone regeneration[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(35): 5702-5708.
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1.1.7 检索策略 以中国知网数据库为例,见图1。
1.1.8 检索文献量 初步共检索出401篇,其中PubMed数据库255篇,中国知网数据库122篇,万方数据库24篇,最后纳入105篇。
1.4 文献质量评估和数据的提取 共检索到401篇,其中PubMed数据库255篇,中国知网数据库122篇,万方数据库24篇。排除重复性研究、内容较旧或相关性较差的文献,纳入105篇符合标准的文献进行综述,其中PubMed数据库83篇,中国知网数据库22篇,见图2。
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文题释义:
小檗碱:是一种具有骨诱导作用的天然异喹啉类化合物,具有替代骨组织工程中生长因子的潜力。文章从小檗碱促骨形成机制、安全性研究和骨组织工程中的应用3个大方面介绍小檗碱的促骨生成作用,其中详细介绍了其成骨机制,包括促骨形成信号通路及对成骨细胞作用、破骨细胞作用、抗骨质疏松症和在高糖、感染及炎症等特殊条件下的促成骨作用,在此基础之上总结了其生物安全性研究和在骨组织工程领域中的发展近况,为小檗碱在骨组织工程领域中的应用提供理论指导。
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