中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (2): 295-301.doi: 10.12307/2023.863
• 组织构建综述 tissue construction review • 上一篇 下一篇
王京峰,文登台,王士杰,高颖晖
收稿日期:
2022-11-24
接受日期:
2023-01-04
出版日期:
2024-01-18
发布日期:
2023-06-30
通讯作者:
文登台,博士,副教授,硕士研究生导师,鲁东大学,体育学院,山东省烟台市 264025
作者简介:
王京峰,男,1997年生,山东省泰安市人,汉族,鲁东大学在读硕士研究生,主要从事运动人体科学研究。
基金资助:
Wang Jingfeng, Wen Dengtai, Wang Shijie, Gao Yinghui
Received:
2022-11-24
Accepted:
2023-01-04
Online:
2024-01-18
Published:
2023-06-30
Contact:
Wen Dengtai, PhD, Associate professor, Master’s supervisor, College of Physical Education, Ludong University, Yantai 264025, Shandong Province, China
About author:
Wang Jingfeng, Master candidate, College of Physical Education, Ludong University, Yantai 264025, Shandong Province, China
Supported by:
摘要:
文题释义:
Atg基因(蛋白):Atg基因在进化过程中是高度保守的,从酵母和果蝇到脊椎动物和人类中都可以找到参与自噬的同源基因,Atg蛋白之间相互作用于自噬的连续过程中,参与自噬过程的调节。
背景:运动作为一种可行的非药物治疗方法,有可能逆转随着年龄增长而恶化的骨骼肌衰老。自噬在骨骼肌衰老过程中的作用是不可缺少的。在骨骼肌衰老期间,参与调节自噬的Atg基因以或促进或抑制的方式调节自噬过程,以改善骨骼肌的生理形态。然而自噬在运动调节骨骼肌衰老中的具体分子机制仍令人困惑。
目的:通过对该领域文献的回顾,寻找运动中自噬机制对骨骼肌衰老影响的一般规律。结果与结论:Atg家族介导的自噬对于延缓骨骼肌衰老是不可或缺的。参与调节自噬的Atg基因以或促进或抑制的方式调节自噬过程,以改善骨骼肌的生理形态及功能。不同的运动模式,如开始运动的年龄、时间或者强度,可能对自噬相关蛋白的表达有异质性的影响,但长期的有氧运动可以调节Atg相关蛋白,诱导骨骼肌自噬,并延缓肌肉质量的损失。
https://orcid.org/0000-0002-9581-6381(王京峰)
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程
中图分类号:
王京峰, 文登台, 王士杰, 高颖晖. Atg介导的自噬、运动和骨骼肌衰老[J]. 中国组织工程研究, 2024, 28(2): 295-301.
Wang Jingfeng, Wen Dengtai, Wang Shijie, Gao Yinghui. Atg-mediated autophagy, exercise and skeletal muscle aging[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(2): 295-301.
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1.1.7 检索策略 以使用WOS数据库检索文献为例,在确定文章方向之后,围绕文章关键词“Atg基因、自噬、运动和骨骼肌衰老”进行关键词的组合搜索,例如输入核心词“Autophagy and skeletal muscle aging”若搜索文献量过少,则选择减少限制词aging再次搜索相关领域文献。在确定文献阅读范围之后,通过文献的阅读逐步排除非相关文献。在阅读期间可利用CitespaceV软件将所确定文献进行进一步的精简,更精准地把握相关文献与所撰写文章的契合度。具体操作步骤:在WOS数据库中输入关键词“Autophagy and skeletal muscle aging”搜索文献,将所有文献以纯文本形式,以及限定全记录和参考文献,下载并以download.txt的格式保存到已经建好的date文件夹中,利用CitespaceV软件将文献数据做可视化分析,锁定需要浏览的与文章相关的文献范围。图1,2。
1.1.8 检索文献量 共检索到1 203篇,其中CNKI数据库检索到203篇、WOS数据库检索到1 000篇。
1.3 数据的提取 1 203篇剔除重复文献后得到816篇,其中国内文献174篇、国外文献642篇;阅读文题排除404篇,初筛得到412篇;浏览全文后排除306篇,复筛纳入106篇;最后进行综合分析,纳入88篇文献进行总结。文献筛选流程图,见图3。
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文题释义:
Atg基因(蛋白):Atg基因在进化过程中是高度保守的,从酵母和果蝇到脊椎动物和人类中都可以找到参与自噬的同源基因,Atg蛋白之间相互作用于自噬的连续过程中,参与自噬过程的调节。Atg家族介导的自噬对于延缓骨骼肌衰老是不可或缺的。参与调节自噬的Atg基因以或促进或抑制的方式调节自噬过程,以改善骨骼肌的生理形态及功能。目前仍有Atg家族的部分基因在骨骼肌中的作用还未得到探究,尤其是在运动干预之下,例如Atg2、Atg10、Atg17、Atg101等。运动作为一种可行的非药物治疗方法可以改善骨骼肌随年龄增长的恶化,但是一些特殊疾病除外,如糖尿病、癌症等。不同的运动模式,如开始运动的年龄、时间或者强度,可能对自噬相关蛋白的表达有异质性的影响,但长期的有氧运动可以调节Atg相关蛋白,诱导骨骼肌自噬,并延缓肌肉质量的损失。从长远来看,精确控制运动量对于最大限度地发挥运动的益处至关重要。然而运动引起的适宜自噬的阈值还有待实验的推敲,而对于Atg基因分子机制的实验探究可能会为该量度提供一个很好的方向。相信在不久的将来针对于Atg家族基因联合运动在骨骼肌衰老中的研究将会陆续的展开,Atg家族体系的作用将会不断完善,这将会是一个充满期待且有趣的过程。
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