中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (12): 1811-1816.doi: 10.12307/2023.135

• 组织工程骨材料 tissue-engineered bone • 上一篇    下一篇

聚乙二醇衍生物水凝胶为载体的力生长因子E肽仿生骨基质材料性能

彭  坤   

  1. 重庆医药高等专科学校，重庆市  401331
  • 收稿日期:2022-03-14 接受日期:2022-05-17 出版日期:2023-04-28 发布日期:2022-07-30
  • 通讯作者: 彭坤，博士，副教授，重庆医药高等专科学校，重庆市 401331
  • 作者简介:彭坤，男，1982年生，重庆市人，博士，副教授，主要从事生物医学、智能医学的研究。
  • 基金资助:
    重庆市自然科学基金(cstc2018jcyjAX0828)，项目负责人：彭坤；第三批重庆市高等学校青年骨干教师资助计划(渝教人发[2016]41号)，项目负责人：彭坤

Properties of force growth factor E peptide bionic bone matrix with polyethylene glycol derivative hydrogel as a carrier

Peng Kun   

  1. Chongqing Medical and Pharmaceutical College, Chongqing 401331, China
  • Received:2022-03-14 Accepted:2022-05-17 Online:2023-04-28 Published:2022-07-30
  • Contact: Peng Kun, MD, Associate professor, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China
  • About author:Peng Kun, MD, Associate professor, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China
  • Supported by:
    the Natural Science Foundation of Chongqing, No. cstc2018jcyjAX0828 (to PK); the Third Batch of Funding Plan for Young Backbone Teachers in Chongqing Institutions of Higher Learning, No. [2016]41 (to PK)

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摘要：

文题释义：
仿生骨基质材料：是一种具有天然骨基质类似的结构(结构仿生)和功能(功能仿生)的骨基质材料，可在体内降解，无毒性及其他不良反应，能够促进成骨细胞在材料表面形成新骨组织，是细胞生长的外微环境，能够提供必要的支撑条件和细胞因子。
力生长因子E肽：属于胰岛素样生长因子1(IGF-1)的选择性剪接变体，成熟后的IGF-1表现出3种亚型，即IGF-1Ea，IGF-1Eb和IGF-1Ec，其中IGF-1Ec就称之为力生长因子(MGF)，属于典型的应力敏感型生物活性物质。MGF在氨基端具有特殊的E肽结构，氨基端多出了氨基酸序列，出现了延伸肽(E肽)，即特异性的MGF氨基酸序列结构，也称之为MGF-Ct24E。

背景：具有生物学功能的骨修复材料一直是生物医学工程领域的研究热点。
目的：从分子结构仿生与生物功能仿生的需求出发，研制具有仿生空间结构和仿生功能的仿生骨基质材料。
方法：以氨基封端的聚乙二醇和均苯四甲酸酐为反应物，通过调节丁二胺加入的量(15，30，45，60 µL)，获得4种性状的聚乙二醇衍生物水凝胶，向4种水凝胶中分别加入基于聚乳酸和力生长因子的生物活性物质(MGF-Ct24E-MPLA-EG-g-HAP)，采用溶剂共混法制备生物活性仿生骨基质材料(PAPI-BDA/MGF-Ct24E-MPLA-EG-g-HAP)。表征生物活性仿生骨基质材料的生物活性物质负载率、孔隙率、表面形貌、亲/疏水性能、吸水率及微观形貌。
结果与结论：①在仿生骨基质材料制作过程中，在pH=5.8的PBS中，随着丁二胺加入量的增加，仿生骨基质材料中生物活性物质负载量呈先增多再减少的现象，当丁二胺加入量为30 µL时生物活性物质负载量最高；在pH=3.5-7.4的PBS中，仿生骨基质材料(丁二胺加入量为30 µL)中生物活性物质负载量呈减少趋势，当pH值增加到9.8时，仿生骨基质材料中生物活性物质负载量基本保持稳定；②在相同pH值的PBS中，随着丁二胺加入量的增加，仿生骨基质材料的孔隙率降低；当丁二胺加入量相同时，随着PBS pH值的增大，仿生骨基质材料的孔隙率呈先降低后升高的趋势；③在pH=5.8的PBS中，随着丁二胺加入量的增加，仿生骨基质材料的静态接触角减小，吸水率增加；④扫描电镜下可见，仿生骨基质材料呈连续开放孔状结构，孔间相互贯通，孔径分布230-690 nm，且孔径大小与丁二胺加入量呈反向相关，其中丁二胺加入量为30 µL制备的仿生骨基质材料类似天然骨基质结构。

https://orcid.org/0000-0002-4703-7893(彭坤)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 聚乙二醇衍生物, 丁二胺, 水凝胶, 聚乳酸, 力生长因子E肽, 仿生骨基质材料

Abstract: BACKGROUND: Bone repair materials with biological functions have always been a research focus in biomedical engineering.
OBJECTIVE: To develop bionic bone matrix material with bionic space structure and bionic function based on the requirements of molecular structure biomimetic and biological function biomimetic. 
METHODS: Using amino-terminated polyethylene glycol and pyromellitic anhydride as reactants, four kinds of polyethylene glycol derivative hydrogels were obtained by adjusting the amount of butanediamine added (15, 30, 45, 60 µL). Bioactive substances based on polylactic acid and force growth factor (MGF-Ct24E-MPLA-EG-g-HAP) were added to the four hydrogels separately. The solvent method was used to prepare bioactive biomimetic bone matrix material (PAPI-BDA/MGF-Ct24E-MPLA-EG-g-HAP). The bioactive material loading rate, porosity, surface morphology, hydrophilic/hydrophobic properties, water absorption and microscopic morphology of the bioactive bionic bone matrix materials were characterized.
RESULTS AND CONCLUSION: (1) In the production process of the bionic bone matrix material, in PBS with pH=5.8, with the increased amount of butanediamine added, the loading of bioactive substances in the bionic bone matrix material first increased and then decreased. The loading of bioactive substances was the highest when the amount was 30 µL; in PBS with pH=3.5-7.4, the loading of bioactive substances in the biomimetic bone matrix material (the addition amount of butanediamine was 30 µL) showed a decreasing trend. When the pH value increased to 9.8, the loading of bioactive substances in the bionic bone matrix material remained basically stable. (2) In PBS with the same pH value, the porosity of the bionic bone matrix material decreased with the increase of the addition amount of butanediamine. When the addition amount of butanediamine was the same, with the increase of the pH value of PBS, the porosity of the bionic bone matrix material first decreased and then increased. (3) In PBS with pH=5.8, with the increase of the addition of butanediamine, the static contact angle of the bionic bone matrix material decreased and the water absorption increased. (4) It can be seen under the scanning electron microscope, the bionic bone matrix material has a continuous open pore structure, the pores are interconnected, the pore size distribution is 230-690 nm, and the pore size is inversely related to the amount of butanediamine added. The bionic bone matrix material prepared by adding butanediamine in an amount of 30 µL is similar to the structure of natural bone matrix.  

Key words: polyethylene glycol derivatives, butanediamine, hydrogel, polylactic acid, force growth factor E peptide, bionic bone matrix material

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