中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (25): 4034-4045.doi: 10.12307/2022.411
• 干细胞综述 stem cell review • 上一篇 下一篇
闵子洋,穆妮热·艾力,郑耘昊,曾幸芷,边楠雁,邓双珊,谢 静
收稿日期:
2020-11-20
接受日期:
2021-03-06
出版日期:
2022-09-08
发布日期:
2022-01-26
通讯作者:
谢静,教授,博士生导师,四川大学华西口腔医学院,口腔疾病研究国家重点实验室,四川省成都市 610041
作者简介:
闵子洋,男,2000年生,云南省玉溪市人,汉族,四川大学华西口腔医学院本科生在读,现随谢静教授进行基础医学(主要是组织工程支架力学性质方面)方向的学习研究。
基金资助:
Min Ziyang, Munire·Aili, Zheng Yunhao, Zeng Xingzhi, Bian Nanyan, Deng Shuangshan, Xie Jing
Received:
2020-11-20
Accepted:
2021-03-06
Online:
2022-09-08
Published:
2022-01-26
Contact:
Xie Jing, Professor, Doctoral supervisor, State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
About author:
Min Ziyang, State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
Supported by:
摘要:
文题释义:
细胞外基质力学微环境:组织工程进行细胞培养时,支架材料为细胞提供的生存环境称细胞外基质微环境,而此处的“力学”强调了材料界面的硬度、孔隙率、拓扑结构和亲/疏水等力学性质。
背景:细胞外基质力学微环境通过调控细胞黏附、迁移、增殖和分化等,在人体器官发育、生理功能维持和疾病发生等方面发挥了重要作用。在再生医学和干细胞疗法中,基质力学微环境能引导植入细胞的存活、生长、增殖与分化,对调控植入细胞的行为发挥了重要的作用,对组织再生和干细胞治疗的成功率也起到关键作用。
目的:综述细胞外基质微环境的力学信号对细胞行为包括细胞骨架重建、迁移、增殖、分化和细胞间交流的影响,从中阐明现有的分子调控机制,以期为组织工程和干细胞疗法中细胞与其力学微环境相互作用的实践性转化提供理论支撑。
方法:检索PubMed数据库、万方数据库、CNKI中国期刊全文数据库收录的相关文献。英文检索词为“cytoskeleton,cell spreading,cell migration,cell proliferation,cell differentiation,cell communication,mechanotransduction,stiffness of substrate,surface topography,extracellular matrix,matrix”,中文检索词为“细胞骨架,细胞扩展,细胞迁移,细胞增殖,细胞分化,细胞交流,机械传导,基质硬度,表面形貌,细胞外基质,基质”,最终纳入161篇文献进行归纳总结。
结果与结论:细胞外基质各种力学信号,如材料界面硬度、拓扑结构和亲/疏水性等,从力学识别、力学-化学信号转导、信号通路级联、下游蛋白活化、转录启动到蛋白表达来调控细胞的扩展、增殖、迁移、分化、交流和其他各种特殊生理性质,这对组织工程中细胞的定向培养和临床医学中的细胞靶向治疗具有重要意义。
https://orcid.org/0000-0001-8156-0322 (谢静)
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
中图分类号:
闵子洋, 穆妮热·艾力, 郑耘昊, 曾幸芷, 边楠雁, 邓双珊, 谢 静. 细胞外基质力学微环境与细胞间相互作用的机制与特征[J]. 中国组织工程研究, 2022, 26(25): 4034-4045.
Min Ziyang, Munire·Aili, Zheng Yunhao, Zeng Xingzhi, Bian Nanyan, Deng Shuangshan, Xie Jing. Mechanism and characteristics of mechanical microenvironment of extracellular matrix and intercellular interaction[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(25): 4034-4045.
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文题释义:
细胞外基质力学微环境:组织工程进行细胞培养时,支架材料为细胞提供的生存环境称细胞外基质微环境,而此处的“力学”强调了材料界面的硬度、孔隙率、拓扑结构和亲/疏水等力学性质。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
目前,组织工程已在生命科学和医学领域展现出不可忽视的潜力和影响,成功取得临床转化的研究更是治愈或挽救了无数患者。支架材料的生物力学评价/制备方法、干细胞筛选、外泌体作用、生物活性因子-细胞-细胞外基质的相互作用、数字化/人工智能在组织工程中的交叉运用等日益成为组织工程未来研究的热点,而该综述正是立足于生物微环境、机械微环境和细胞与材料基质间的相互作用这3大创新点,以力学信号组成和细胞受影响的生理功能为分类依据,阐述了细胞外基质力学微环境与细胞的相互作用。经检索,尚未有文献从此角度出发综述各种力学信号对细胞各项生理活动的影响,由此侧面证明了文章的独特与创新性,以期对未来组织工程方面的研究及其分子生物学机制提供参考。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
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