中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1253-1256.doi: 10.3969/j.issn.1673-8225.2010.07.026

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

131 I-泡球蚴多抗在泡球蚴大鼠体内的放射免疫显像

孙晓琰1，秦永德1，王  昕2，3，王赛岗1，吕  洁1，谢  彬1，刘立水1，李肖红1   

  1. 1新疆医科大学第一附属医院核医学科，新疆维吾尔自治区乌鲁木齐市  830054；2四川大学华西基础与法医学院寄生虫教研室，四川省成都市  610041；3新疆医科大学基础医学院寄生虫教研室，新疆维吾尔自治区乌鲁木齐市  830054
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 通讯作者: 王 昕，博士，四川大学华西基础与法医学院寄生虫教研室，四川省成都市 610041；新疆医科大学基础医学院寄生虫教研室，新疆维吾尔自治区乌鲁木齐市830054 joneke55@yahoo.com.cn
  • 作者简介:孙晓琰★，女，1980年生，河北省定兴县人，汉族，新疆医科大学在读硕士，医师，助教，主要从事肿瘤核医学的研究。 zy313xnlb@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30660045)。

Radioimmunoimaging of 131 I-labelled anti-alveolar hydatid polyclonal antibodies in rats with Echinococcus multilocularis in vivo

Sun Xiao-yan1, Qin Yong-de1, Wang Xin 2,3, Wang Sai-gang1, Lü Jie1, Xie Bin1, Liu Li-shui1, Li Xiao-hong1   

  1. 1 Department of Nuclear Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumuqi  830054, Xinjing Uygur Autonomous Region, China; 2 Department  of Parasitology, College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu  610041, Sichuan Province, China; 3 Department of Parasitology and Microbiology, Xinjiang Medical University, Urumuqi  830054, Xinjing Uygur Autonomous Region, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Wang Xin, Doctor, Department of Parasitology, College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China; Department of Parasitology and Microbiology, Xinjiang Medical University, Urumuqi 830054, Xinjing Uygur Autonomous Region, China joneke55@yahoo.com.cn
  • About author:Sun Xiao-yan★, Studying for master’s degree, Physician, Teaching assistant, Department of Nuclear Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumuqi 830054, Xinjing Uygur Autonomous Region, China zy313xnlb@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30660045*

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摘要：

背景：泡球蚴病早期不易被发现，各种影像及免疫试验手段不能降低其误诊率。
目的：观察131 I-泡球蚴多抗在泡球蚴大鼠模型的生物学分布及放射免疫显像。
方法：36只Wistar大鼠随机分为3组，每组12只。A组：肝带虫大鼠；B组：正常大鼠；C组：肝带虫大鼠。A、B组大鼠腹腔注射131 I-泡球蚴多抗1 mL(37 MBq)，C组大鼠腹腔注射131 I 1 mL(37 MBq)，注射后24，48，72，96 h进行ECT静态显像，测定泡球蚴感兴趣区值。96 h后测量3组大鼠各组织器官单位质量每分钟放射性计数，A组大鼠计算虫/非虫比值。
结果与结论：A组大鼠肝脏接种泡球蚴部位可见放射性分布，解剖后观察位置相符，72 h泡球蚴感兴趣区值最高，显像效果最好。B组和C组大鼠肝区均未见明显放射性分布，3组大鼠均见甲状腺部位及胃部有放射性聚集。96 h后处死3组大鼠，A组大鼠体外放射性计数测定，泡球蚴虫体为(18.610±3.401)×103 cpm/g，高于体内除甲状腺及胃外的脏器，差异具有显著性意义(P < 0.001)。将泡球蚴组织与其他器官比较计算虫/非虫比值可以看出，除甲状腺和胃组织其余组织虫/非虫值均大于1.28。3组大鼠除虫体组织外，其余各组织器官的单位质量脏器每分放射性计数差异无显著性意义(P > 0.05)。证实131 I-泡球蚴多抗能成功作用于泡球蚴并与之结合。

关键词: 泡状棘球蚴, 多克隆抗体, 放射免疫显像, 大鼠, 131Ⅰ

Abstract:

BACKGROUND: Echinococcosis alveolaris were not easily discovered in prophase. Although used various kinds of imaging and immunity test, misdiagnosis rate of echinococcosis alveolaris was still high.
OBJECTIVE: To study radioimmunoimaging of 131 I-labelled anti-alveolar hydatid polyclonal antibody in the rat model with Echinococcus multilocularis in vivo.
METHODS: Totally 36 Wistar rats were randomly divided into 3 groups. A group included 12 alveolar echinococcosis rats, B group included 12 normal rats, C group included 12 alveolar echinococcosis rats. Rats in the A and B group were injected intraperitoneally 1 mL 131 I-labelled anti-alveolar echinococcosis polyclonal antibodies (37 MBq), those in the C group were injected intraperitoneally 1 mL 131 I (37 MBq). All rats were imaged 24, 48, 72, 96 hours later with ECT to measure region of interest (ROI) of alveolar hydatid. The unit weight radioactive counts per minute of three group rats organs were measured after 96 hours, and the targetto-nontarget (T/NT) ratios were calculated in A group rats.
RESULTS AND CONCLUSION: In A group rat inoculated right lobe of liver alveolar echinococcosis, radioactive parts of the distribution could be seen. And ROI of alveolar echinococcosis were the highest at 72 hours. After dissection the locations were in line, and it was no significant radioactive liver distribution in B and C group. Rats were sacrified after 96 hours, in vitro determination of radioactive counts show that A group echinococcus multilocularis was (18.610±3.401)×103 cpm/g, which was greater than organs except thyroid and gastric tissues (P < 0.001). The rest of organize T/NT ratios were greater than 1.28 apart from the thyroid and gastric tissues. The difference of unit weight radioactive counts per minute of three group had no obviously significance except the polypide tissues (P > 0.05). This proves that 131 I-labelled anti-alveolar echinococcosis polyclonal antibody successfully combinated with alveolar echinococcosis.

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