中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1248-1252.doi: 10.3969/j.issn.1673-8225.2010.07.025

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

OX40L在C57BL/6与BALB/c小鼠组织的差异性表达及分析

韩学府，王  飞，朱舒舒，徐晋妉，张寄南，杨  笛   

  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 通讯作者: 杨 笛，研究员，博士生导师，南京医科大学第一附属医院心血管病研究所，江苏省南京市 210029 dyang@njmu.edu.cn
  • 作者简介:韩学府★，男，1983年生，山东省潍坊市人，汉族，南京医科大学第一附属医院在读硕士，主要从事分子心脏病学研究。 xf-han@hotmail.com
  • 基金资助:

    国家自然科学基金资助项目(30770894)；江苏省医学重点人才基金资助项目(RC2007037)。

Differential expression of OX40L in C57BL/6 and BALB/c mouse tissues

Han Xue-fu, Wang Fei, Zhu Shu-shu, Xu Jin-dan, Zhang Ji-nan, Yang Di   

  1. Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Yang Di, Researcher, Doctoral supervisor, Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China dyang@njmu.edu.cn
  • About author:Han Xue-fu★, Studying for master’s degree, Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China xf-han@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30770894*; Advanced Training Fund for Medicine of Jiangsu Province, No. RC2007037*

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被引次数

1

摘要：

背景：有研究发现C57BL/6小鼠对动脉粥样硬化易感，而BALB/c小鼠却对动脉粥样硬化不易感。OX40L的表达情况与动脉粥样硬化的狭窄程度和心肌梗死的严重度相关，其在两种品系小鼠中是否存在表达差异？
目的：分析OX40L在BALB/c和C57BL/6小鼠心脏、大脑、肾脏、骨骼肌和脾脏组织的表达差异。
方法：取C57BL/6和BALB/c小鼠心脏、大脑、肾脏、骨骼肌和脾脏组织，以Trizol提取总RNA， RIPA Buffer提取组织总蛋白。采用RT-PCR和Western Blot方法检测两种品系小鼠心脏、脑、肾脏、脾脏和骨骼肌的OX40L mRNA和蛋白的表达。OX40L在两种品系小鼠不同器官间的表达差异。
结果与结论：RT-PCR结果显示，C57BL/6小鼠心脏OX40L mRNA表达显著高于BALB/c小鼠(P < 0.05)，脾脏OX40L mRNA表达明显低于BALB/c小鼠(P < 0.05)，两种品系小鼠大脑、肾脏及骨骼肌OX40LmRNA表达差异无显著性意义；Western Blot结果显示，两种品系小鼠OX40L的蛋白表达均在心脏最高；C57BL/6小鼠心、脑及肾OX40L蛋白表达均显著高于BALB/c小鼠(P < 0.05)，两种品系小鼠骨骼肌和脾脏OX40L蛋白表达差异无显著性意义。两个品系小鼠OX40L mRNA的表达水平与蛋白表达水平不完全一致。C57BL/6小鼠心脏中OX40L mRNA转录水平较BALB/c高，但在脾脏中表达量较后者低；C57BL/6小鼠心脏、大脑和肾脏OX40L蛋白水平均较BALB/c小鼠高；两种品系小鼠之间的表达差异提示OX40L可能与C57BL/6小鼠易感动脉粥样硬化有关。

关键词: OX40L, mRNA , 蛋白表达, 组织工程, 动脉粥样硬化

Abstract:

BACKGROUND: Previous studies found that C57BL/6 mouse was susceptible to atherosclerosis, while BALB/c mouse was resistant to atherosclerosis. The stenosis of the culprit vessel and the severity of myocardial infarction were correlated to the levels of OX40L expression. Whether OX40L has differential expression between C57BL/6 and BALB/c mouse was not identified.
OBJECTIVE: To observe the differential expression of OX40L mRNA and protein in C57BL/6 and BALB/c mouse.
METHODS: Total RNA and protein were extracted by Trizol and RIPA Buffer from heart, brain, kidney, skeletal muscle and spleen tissues of C57BL/6 and BALB/c mice. RT-PCR and Western Blot were used to detect OX40L mRNA and protein expression in heart, brain, kidney, spleen and skeletal muscle of two kinds of mice. The differential expression of OX40L mRNA and protein between C57BL/6 and BALB/c mice was observed.
RESULTS AND CONCLUSION: RT-PCR results showed that the mRNA expression level of OX40L in heart of C57BL/6 mice mouse was significantly higher than BALB/c mice (P < 0.05); the mRNA expression level of OX40L in spleen of BALB/c mice was significantly higher than C57BL/6 mice (P < 0.05). There were no significant differences in the brain, kidney and skeletal muscle between these two strains. The results of Western Blot showed that the protein expression level of OX40L in heart, brain and kidney of C57BL/6 mice were significantly higher than BALB/c mice (P < 0.05). There were no significant differences in skeletal muscle and spleen between these two strains. The OX40L mRNA transcription level in heart was higher in C57BL/6 mouse than BALB/c mouse, while the expression in spleen was lower than the latter. The OX40L protein levels in C57BL/6 mouse heart, brain and kidney were higher than BALB/c mouse. The differences of OX40L expression between the two strains of mice indicated that OX40L may promote to C57BL/6 mouse susceptible to atherosclerosis.　

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