中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1244-1247.doi: 10.3969/j.issn.1673-8225.2010.07.024

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

强直性脊柱炎患者外周血可溶性CD146的表达及意义

薛  勤，汪年松，高许萍，范  瑛，张晓光，唐令诠   

  1. 上海交通大学附属第六人民医院肾脏风湿科，上海市  200233
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 作者简介:薛 勤★，女，1964年生，江苏省常州市人，汉族，1989年上海第二医科大毕业，硕士，副主任医师，主要从事强直性脊柱炎、其他风湿病及肾脏病的研究。 xueqinkcb@126.com

Significance and expression of soluble CD146 in peripheral blood from patients with ankylosing spondlitis

Xue Qin, Wang Nian-song, Gao Xu-ping, Fan Ying, Zhang Xiao-guang, Tang Ling-quan   

  1. Department of Nephrology and Rheumatology, the Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai  200233, China
  • Online:2010-02-12 Published:2010-02-12
  • About author:Xue Qin★, Master, Associate chief physician, Department of Nephrology and Rheumatology, the Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China xueqinkcb@126.com

PDF

458

被引次数

11

摘要：

背景：研究发现在炎症因子的刺激下内皮细胞和活化的T细胞表面表达CD146显著增加，故推测CD146可能参与了组织的炎症反应。
目的：观察强直性脊柱炎患者外周血可溶性CD146的表达水平和临床意义。
方法：选择上海交通大学附属第六人民医院住院的强直性脊柱炎患者62例，分为两组。活动期组46例，非活动组16例。同时选择同期医院健康职工和大学生20名为对照组。在强直性脊柱炎患者初诊时评估BASDAI、BASFI、强直性脊柱炎患者总体评分(PGA)、夜间痛、总体背痛评分、晨僵时间并测定红细胞沉降率、C-反应蛋白水平。应用ELISA法检测62例强直性脊柱炎患者与20例正常人外周血可溶性CD146的表达水平、魏氏法测定血沉及免疫比浊法测定C-反应蛋白。对强直性脊柱炎患者可溶性CD146与各项检测指标相关分析。
结果与结论：强直性脊柱炎患者的外周血清可溶性CD146的表达水平较正常对照组显著增高(P < 0.05)；活动期强直性脊柱炎患者可溶性CD146的表达水平较非活动期和正常对照组显著增高(P < 0.05)；强直性脊柱炎患者血清可溶性CD146的表达水平与BASDAI 呈正相关(P < 0.05)；累及外周关节型者血清可溶性CD146较单独中轴型强直性脊柱炎患者和正常对照组显著增高(P < 0.05)。结果表明，外周可溶性CD146表达水平的增加与强直性脊柱炎患者疾病的活动性有关，其可能参与了强直性脊柱炎的发病过程。

关键词: 脊柱炎, 强直性, 黏附分子, 可溶性CD146, 炎症反应, 外周血

Abstract:

BACKGROUND: Research has been reported that serum soluble CD146 (sCD146) expression was improved on the surface of endothelial cells and activated T cells by the stimulation of inflammatory factor. Therefore, it predicts that CD146 may participate in inflammatory reaction of tissue.
OBJECTIVE: To investigate the expression and clinical significance of serum sCD146 in peripheral blood from patients with ankylosing spondylitis.
METHODS: A total of 62 patients with ankylosing spondylitis were selected from the Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University. All patients were divided into two groups: active group (n=46) and inactive group (n=16); while, 20 healthy subjects were selected as the control group. Indicators including Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Bath Ankylosing Spondylitis Functional Index (BASFI), patient's global assessment (PGA), night pain, visual analogue scale (VAS), morning stiffness time, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were measured in all patients. The serum concentration of sCD146 from 62 patients with ankylosing spondlitis and 20 healthy controls was measured by enzyme-linked immunosorbent assay. Westergren method was used to measure ESR and immunoturbidimetry for CRP. Clinical data of the patients were collected as well. 
RESULTS AND CONCLUSION: sCD146 levels of patients with ankylosing spondlitis were significantly higher than normal control group (P < 0.05). The sCD146 expression in the active group was significantly higher than inactive and normal control groups (P < 0.05). Positive correlations were observed between sCD146 and BASDAI index of patients with ankylosing spondlitis (P < 0.05). The sCD146 levels of ankylosing spondlitis patients with peripheral joint involvement were significantly higher than the patients with axial involvement alone or the normal controls (P < 0.05).
The expression level of sCD146 in peripheral blood was positively correlated with disease activities of patients with ankylosing spondlitis. It may play important roles in the pathogenesis in ankylosing spondlitis.

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