Preparation of the surface patterned polyacrylamide-acrylic
hydrogel

doi:10.3969/j.issn.2095-4344.2230

Abstract

Abstract:

BACKGROUND: Polyacrylamide hydrogels have good biocompatibility, but their mechanical properties are poor, which affect their application in the field of biomaterials.

OBJECTIVE: To prepare the polyacrylamide-acrylic hydrogels with a particular size by micromolding graphical imprinting.

METHODS: Polyacrylamide-acrylic hydrogel was prepared by sequentially mixing different volumes of polyacrylamide solution, acrylic acid and ammonium persulfate solution into an orifice plate containing a micromolded patterned seal. Group A: polyacrylamide solution 1.4 mL, acrylic acid 0.1 mL; group B: polyacrylamide solution 1.3 mL, acrylic acid 0.2 mL; group C: polyacrylamide solution 1.2 mL, acrylic acid 0.3 mL; group D: polyacrylamide solution 1.1 mL, acrylic acid 0.4 mL; group E: polyacrylamide solution 1.0 mL, acrylic acid 0.5 mL; group F: polyacrylamide solution 0.9 mL, acrylic acid 0.6 mL. Six groups of ammonium persulfate solution were all 50 μL. The patterned structure of the hydrogel was observed under a light microscope. The mechanical properties of the hydrogel were examined by an electronic universal testing machine.

RESULTS AND CONCLUSION: Light microscope showed that the stripes on the surface of each group of hydrogels were clearly visible. The addition of acrylic acid effectively improved the mechanical properties of hydrogels. As the proportion of acrylic acid increased, the mechanical properties of hydrogels gradually increased. These results suggest that polyacrylic acid/acrylamide hydrogel has good mechanical properties and is expected to have good application prospects in the field of tissue engineering damage repair.

Key words:

"> surface patterned, polyacrylamide, acrylic acid, hydrogel, mechanical property, tissue engineering, biomaterials, preparation

CLC Number:

Li Shuhua, Li Meilan. Preparation of the surface patterned polyacrylamide-acrylic hydrogel[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(10): 1553-1556.

Figures/Tables 3

在实验初期，印章的使用方法定为将带有图案的一面倒扣至多孔板上，并用胶带将印章固定在孔板上，但由于印章的疏水性质，丙烯酸、聚丙烯酰胺、过硫酸铵混合液并没有流进孔板与印章之间的缝隙，水凝胶没有得到有效的图案化。这主要是由于印章的疏水结构，在加热过程中丙烯酸、聚丙烯酰胺、过硫酸铵的混合液会自动疏离印章上带有图案的地方，这样在水凝胶成胶之后会出现“空心”的现象。之后将印章带有图案的一面朝上，另一面用双面胶紧贴多孔板单孔的中心，用手指压紧以防止在加热过程中印章漂浮，这样水凝胶可以很好地将印章覆盖，不会出现水凝胶中间产生空洞的现象，使水凝胶很好的图案化。在加热过程中丙烯酸由于遇水蒸气而放热，导致还未成胶的混合液产生大量气泡，而在产生气泡的过程中水凝胶也在不断地成胶凝固，这时产生的气泡便会残留在成型的水凝胶中，气泡的残留对水凝胶的图案化产生了很大影响，因此此次实验对此采取的措施是：在加热过程中对其进行抽真空处理，选择的时段是在将要开始产生气泡的时间点，大约在开始加热后的20 min。抽真空时将多孔板的板盖去掉，之后开始抽真空，重复3次抽真空后将多孔板的盖子盖回原处，这样处理过，水凝胶中的气泡大幅减少。水凝胶在加热过程中，加热时间越长水蒸气在多孔板的盖子上凝结的越多，以至于水滴滴落造成丙烯酸放热，使孔板变形。加热温度是影响水凝胶成型时长的因素之一，加热温度越高水蒸气蒸发越快，水滴就会越多。第二个因素是交联剂(过硫酸铵)的浓度，交联剂浓度越高成胶的速度则越快。根据以上2点，此次实验对传统水凝胶的制备方法加以改进，将加热温度确定为55 ℃，将交联剂(过硫酸铵)的用量确定为50 μL。 2.2 复合水凝胶的力学性能检测结果为了测试加入不同体积丙烯酸是否能够改变生物材料的弹性模量，对6种加入不同体积丙烯酸的水凝胶进行了力学分析，在对样品施加相同应力时，不同水凝胶所产生的应变是不同的，其中所表现出的不同正好可以反映出不同水凝胶的力学性能的强弱；当应力不断增加时，样品会发生变形直至断裂，见图2。 "

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