Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (22): 3493-3499.doi: 10.3969/j.issn.2095-4344.3240

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Effect of modification of β-tricalcium phosphate/chitosan hydrogel on growth and mineralization of dental pulp stem cells

Li Xinping, Cui Qiuju, Zeng Shuguang, Ran Gaoying, Zhang Zhaoqiang, Liu Xianwen, Fang Wei, Xu Shuaimei   

  1. Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China
  • Received:2020-08-12 Revised:2020-08-14 Accepted:2020-10-09 Online:2021-08-08 Published:2021-01-19
  • Contact: Zeng Shuguang, Professor, MD, Doctoral supervisor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China
  • About author:Cui Qiuju, MD, Attending physician, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China Li Xinping and Cui Qiuju contributed equally to this article.
  • Supported by:
    the Guangzhou Science and Technology Project, No. 201707010193 (to ZSG); the National Natural Science Foundation of China, No. 81800957 (to XSM); the Hospital Level Project of Stomatological Hospital of Southern Medical University, No. PY2019023 (to CQJ)

Abstract: BACKGROUND: Using the two materials of genipin crosslinking β-tricalcium phosphate and chitosan, we explored an effective and simple synthetic method to form a stable hydrogel material with good biocompatibility. This will lay a foundation for the application of tissue engineering in bone defect repair in the future.
OBJECTIVE: To investigate the effects of β-tricalcium phosphate modified chitosan hydrogel on the growth and mineralization of dental pulp stem cells.
METHODS: Chitosan and β-tricalcium phosphate were selected as raw materials to construct biomimetic bone tissue engineering hydrogel with different ratios. The physical and chemical properties of the hydrogel were tested, including morphology, porosity and release of calcium in the extract. The human dental pulp stem cells were then co-cultured with hydrogel to observe their growth, adhesion and mineralization.
RESULTS AND CONCLUSION: (1) The chitosan hydrogel with β-tricalcium phosphate was more uniform in pore size and more stable in morphology. The hydrogel could release calcium continuously. (2) The hydrogel added with β-tricalcium phosphate showed good biocompatibility after co-culture with dental pulp stem cells, and was able to induce mineralization of dental pulp stem cells without adding osteogenic inducer. (3) The hydrogel obtained in this experiment has good biocompatibility and certain osteoinductivity, which provides a new idea for the preparation of new tissue engineering materials. 

Key words: dental pulp stem cells, β-tricalcium phosphate, chitosan, hydrogel, osteogenesis, mineralization, biocompatibility

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