Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (16): 2488-2493.doi: 10.3969/j.issn.2095-4344.3114
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Zhang Xin1, Lu Ying1, Yao Qingqiang2, Zhu Yishen1
Received:
2020-02-18
Revised:
2020-02-27
Accepted:
2020-06-05
Online:
2021-06-08
Published:
2021-01-07
Contact:
Yao Qingqiang, Professor, Department of Orthopedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China
Zhu Yishen, Professor, The College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu Province, China
About author:
Zhang Xin, Master, Nanjing Tech University, Nanjing 211816, Jiangsu Province, China
Supported by:
CLC Number:
Zhang Xin, Lu Ying, Yao Qingqiang, Zhu Yishen. Preparation and in vitro evaluation of self-assembling peptide hydrogel loaded with doxorubicin[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2488-2493.
2.6 SAPH体外细胞相容性实验 由CCK-8检测结果可知(图8a),20 g/L SAPH浸提液中第1,3,5 天的兔骨髓间充质干细胞增殖高于30,40 g/L SAPH浸提液(P < 0.05);第7天时,由于皿底细胞量高细胞接触抑制,3组间细胞增殖比较差异无显著性意义(P > 0.05);其中培养第1天时,40 g/L SAPH浸提液中的细胞增殖率为86%,有一定的细胞毒性。结合体外释放率结果,选30 g/L SAPH负载DOX进行细胞毒性实验。 2.7 DOX-SAPH细胞毒性实验 根据图8b结果所示, DOX-SAPH浸提液与DOX溶液都能明显抑制MG63细胞的增殖。DOX-SAPH浸提液的IC50值为6.26 mg/L,DOX溶液的IC50值为2.91 mg/L。当DOX的质量浓度<12.50 mg/L时,DOX溶液和DOX-SAPH浸提液的细胞毒性无明显差异(P > 0.05);当DOX质量浓度≥12.50 mg/L时,与DOX-SAPH浸提液相比,DOX溶液对MG63细胞展现出更强的细胞毒性。"
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