Type-2 cannabinoid receptor agonist inhibits titanium particle-induced osteoblast apoptosis

doi:10.3969/j.issn.2095-4344.2016.43.007

Abstract

Abstract:

BACKGROUND: Impaired osteogenesis caused by osteoblast apoptosis is a main reason of aseptic loosening. Type-2 cannabinoid receptor activation can promote osteoblast proliferation and reduce apoptosis.
OBJECTIVE: To investigate the inhibitory effect of HU-308 (type-2 cannabinoid receptor agonist) on the osteoblastic MC3T3-E1 cells co-cultured with titanium particles and its relative mechanism.
METHODS: Mouse MC3T3-E1 cells were cultured in vitro and divided into five groups: cells were cultured in the normal medium as blank control group, cultured in the medium with 2.5 g/L titanium particles as titanium group, cultured in the medium containing 2.5 g/L titanium particles plus 10^-9, 10^-8 and 10-⁷ mol/L HU-308 as low-, medium- and high-concentration groups, respectively. Forty-eight hours later, cell proliferation, apoptosis, levels of reactive oxygen species and mitochondrial membrane potential as well as Caspase-3 activity were assessed.
RESULTS AND CONCLUSION: Compared with the black control group, the cell proliferation activity and the level of mitochondrial membrane potential were significantly reduced and the apoptosis rate, the level of reactive oxygen species and Caspase-3 activity were significantly increased in the titanium group (P < 0.01 or P < 0.05). Compared with the titanium group, the cell proliferation activity and the level of mitochondrial membrane potential significantly increased in the high-concentration group, and the apoptosis rate and Caspase-3 activity significantly decreased in the medium- and high-concentration groups (P < 0.05). These findings demonstrate that HU-308 can effectively improve the impaired osteogenesis induced by titanium particles, probably through modulating reactive oxygen species and elevating mitochondrial membrane potential and Caspase-3 activity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Titanium, Osteoblasts, Apoptosis, Tissue Engineering

CLC Number:

R318

Pei Fang, Zhou Bing, Zheng Wei, Shen Zhen-yu, Wang Chen, Qiu Shang, Guo Kai-jin.

Type-2 cannabinoid receptor agonist inhibits titanium particle-induced osteoblast apoptosis

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(43): 6431-6437.

Figures/Tables 3

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