Correlation of bioactive glass ceramic coating and Hedgehog signaling pathway in osteogenesis

doi:10.3969/j.issn.2095-4344.2016.43.006

Abstract

Abstract:

BACKGROUND: Bioactive glass ceramics (BGC) has been successfully applied as titanium alloy coating in the clinic. However, the correlation between Hedgehog signaling pathway and BGC coating in osteogenesis has not yet been reported.
OBJECTIVE: To explore the correlation of Hedgehog signaling pathways and BGC coating in osteogenesis.
METHODS: The BGC coating was prepared and observed by scanning and transmission electron microscopes. Primary cultured rat bone marrow mesenchymal stem cells were incubated onto the BGC coating. The expressions of bone morphogenetic protein 2 and Gliloma-association oncogene homoglog in the Hedgehog signaling pathway were detected using fluorescent quantitative RT-PCR, western blot assay, and immunofluorescence staining, and the cell migration ability was observed.
RESULTS AND CONCLUSION: Electron microscopes showed that there were no cracks on the smooth BGC coating that had the dense mesoporous structure, and the coating thickness was 350 nm. The mRNA and protein expressions of bone morphogenetic protein 2 and Gliloma-association oncogene homoglog in the BGC group were significantly higher than those in the control group; and the expresssed bone morphogenetic protein 2 and Gliloma-association oncogene homoglog proteins interacted with each other in the process of osteogenesis. The cell migration ability in the BGC group was obviously enhanced compared with the control group. These results indicate that the BGC coating increases the expression of Gliloma-association oncogene homoglog, then further activates the Hedgehog signaling pathway, and finally accelerates the osteoblast proliferation in combination with bone morphogenetic protein 2.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Osteoblasts, Tissue Engineering

CLC Number:

R318

Ni Wei-dong, Fang Long-yun.

Correlation of bioactive glass ceramic coating and Hedgehog signaling pathway in osteogenesis

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(43): 6424-6430.

Figures/Tables 6

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