Biocompatibility of magnetic chitosan microspheres

doi:10.3969/j.issn.2095-4344.2013.42.012

Abstract

Abstract:

BACKGROUND: Chitosan modification for magnetic iron oxide nanoparticles can improve the agglomeration and stability of magnetic iron oxide nanoparticles, and in addition, it can be used for tumor hyperthermia and gene therapy in the future.
OBJECTIVE: To explore the biocompatibility of magnetic chitosan microspheres which have the potential application in tumor hyperthermia and gene therapy.
METHODS: Magnetic iron oxide nanoparticles were prepared using the modified chemical precipitation. Phacoemulsification method was used to add chitosan into magnetic iron oxide nanoparticles in the preparation of magnetic chitosan microspheres. Then, the following tests were performed. (1) Cytotoxicity test: L-929 cells were cultured in 1640 medium, polyacrylamide monomer solution, 100%, 75%, 50%, 25% of magnetic chitosan microsphere extracts. (2) Hemolysis test: Magnetic chitosan microspheres leaching solution, saline and distilled water were added. (3) Micronucleus test: Kunming mice were intraperitoneally injected with magnetic chitosan microspheres suspension containing 5, 3.75, 2.5, 1.25 g/kg iron oxide magnetic fluid, cyclophosphamide and saline, respectively.
RESULTS AND CONCLUSION: Magnetic chitosan microspheres of 200-300 nm in diameter had an increased dispersion effect. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the toxicity of the material on L-929 cell lines belonged to no cytotoxicity. In the hemolysis test, the hemolysis rate of magnetic chitosan microspheres was 0.69% far less than 5%. In the micronucleus test, magnetic chitosan microspheres suspension did not cause DNA fragmentation and aneuploidy, and did not result in genetic toxicological effects produced by micronuclei. The magnetic chitosan microspheres did not appear with cacogenesis and mutagenesis. From the results, magnetic chitosan microspheres are a kind of high biocompatibility material.

Key words: microspheres, chitosan, biocompatible materials, bone matrix

CLC Number:

R318

Wang Zi-yu, Dong Ju, Zhang Dong-sheng . Biocompatibility of magnetic chitosan microspheres[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(42): 7414-7419.

Figures/Tables 4

References

[1] 李广峰,杨建东.壳聚糖纳米粒子基因载体的研究现状[J].中国组织工程研究与临床康复,2011,15(47):8879-8882.
[2] Lin YH,Mi FL,Chen CT,et al.Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery. Biomacromolecules. 2007;8(1):146-152.
[3] 徐缨龙,蒋辉,汪景洲,等.制备肝靶向载体半乳糖基化壳聚糖/ DNA 纳米颗粒转染肝癌细胞SMMC- 7721 的体外实验[J].现代预防医学,2010,37(18):3507-3510.
[4] 陈道桢,李向东,唐秋莎,等. 超顺磁性氧化铁纳米粒子的制备、表征及生物相容性研究[J].山东医药,2012,52(39):1-4.
[5] 张皓,侯欣欣,张东生.磁性纳米材料在肿瘤热化疗中的研究进展[J].东南大学学报:医学版,2012,31(4):483-488.
[6] 杜益群,张东生,倪海燕,等. 肿瘤热疗用Fe3O4 磁性纳米粒的制备及表征[J].电子显微学报,2005,24(6):608-612.
[7] 葛玉卿,张宇,顾宁. 壳聚糖修饰氧化铁磁性纳米颗粒的制备和性能研究[J].功能材料与器件学报,2012,18(2):147-152.
[8] 季业,邵惠萍,郭志猛,等. 超声乳化法制备纳米Fe3O4磁性颗粒及壳聚糖表面改性[J].北京科技大学学报,2011,33(6):751-755.
[9] 周恒,罗聪,黄华. 医用磁性纳米粒的研究进展[J].中国医院药学杂志,2010,30(8):689-692.
[10] 陈朝婷,高峰,陆平,等.磁性壳聚糖纳米介导eNOS 基因转染受损平滑肌细胞初探[J].东南大学学报:医学版,2011,30(6): 827-831.
[11] 何荣芬,袁刚,邓旭晖. 磁性壳聚糖微球研究进展[J].检验医学与临床,2011,8(12):1500-1502.
[12] 颜士岩,张东生,顾宁,等.肿瘤热疗用Fe2O3 纳米磁性粒子的生物相容性研究[J].东南大学学报:医学版,2005,24(1):8-12.
[13] Wang ZY,Song J,Zhang DS.Nanosized As2O3/Fe2O3 complexes combined with magnetic fluid hyperthermia selectively target liver cancer cells. World J Gastroenterol. 2009;15(24):2995-3002.
[14] 杜益群,张东生,倪海燕,等.肿瘤热疗用Fe3O4 磁性纳米粒子的生物相容性研究[J].南京大学学报:自然科学版,2006,43(2): 324-330.
[15] 邹芬,潘一峰,张红,等. 聚乙二醇-聚乙烯亚胺/四氧化三铁纳米磁流体的毒性及其生物相容性[J].中国组织工程研究与临床康复, 2010,14(3):447-451.
[16] 吕晓迎,Kappert HF. 牙科材料细胞毒性评定的新方法(MTT 试验) [J].中华口腔医学杂志,1995,30(6):377-379.
[17] 刘静,张东生. 肿瘤热疗用纳米磁性材料的生物相容性评价方法研究进展[J]. 东南大学学报:医学版,2010,29(5):587-592.
[18] oxide nanoparticle aqueous suspensions to Zebrafish (Danio rerio) early developmental stage. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2008;43(3):278-284.