Function on 3D printing poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/calcium sulfate hemihydrate scaffold integrated chitosan hydrogel coating

doi:10.12307/2022.206

Abstract

Abstract: BACKGROUND: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was a new type of polymer with good biocompatibility and degradability. However, PHBV has strong hydrophobicity, and was not conducive to cell adhesion, growth, migration, and differentiation, which severely limits its application in the field of bone tissue engineering. Thus, it is necessary to further modify it.
OBJECTIVE: To construct PHBV/calcium sulfate hemihydrate (CSH)/chitosan (CS) scaffolds, and explore its characterization and drug release behavior, biological safety, osteogenic properties and antibacterial activity in vitro.
METHODS: PHBV and PHBV/CSH scaffolds with 20 wt% CSH content were prepared by fused deposition technology. The PHBV/CSH scaffolds were immersed in the chitosan solution and dried, and the dried scaffolds were put into ultrapure water to form a chitosan hydrogel on the surface of the scaffolds, termed the PHBV/CSH/CS scaffolds. The surface morphology, cell adhesion, and cell proliferation of PHBV, PHBV/CSH, PHBV/CSH/CS scaffolds were observed. The swelling behavior and simulated drug release behavior of the PHBV/CSH/CS scaffolds were tested. Alizarin red staining was used to evaluate the osteogenic performance of these scaffolds. The spread plate method was used to observe the antibacterial ability of each group of scaffolds against standard Staphylococcus aureus, standard Escherichia coli, clinical Staphylococcus aureus, and clinical Escherichia coli.
RESULTS AND CONCLUSION: (1) The scaffolds all had a uniform and interconnected porous three-dimensional structure, with an average pore size of about 400 μm and a porosity of 60%. (2) The swelling rate of PHBV/CSH/CS scaffold was 56%. When using bovine serum albumin as a model drug, the loading rate of PHBV/CSH/CS scaffold was 57.9% at 1 hour, and the cumulative release at 6 hours was 56.5%. (3) PHBV scaffolds were not conducive to cell adhesion. PHBV/CSH scaffolds improved cell adhesion. PHBV/CSH/CS scaffolds significantly improved cell adhesion. (4) PHBV, PHBV/CSH, PHBV/CSH/CS scaffolds could promote the proliferation of bone marrow mesenchymal stem cells, and there was no obvious cytotoxicity in the three groups. (5) Alizarin red staining experiments showed that the PHBV/CSH/CS scaffold had a stronger effect on the formation of mineralized nodules in bone marrow mesenchymal stem cells than the other two groups. (6) In vitro antibacterial experiments showed that the PHBV/CSH/CS scaffolds were significantly stronger against standard Staphylococcus aureus, standard Escherichia coli, clinical Staphylococcus aureus, and clinical Escherichia coli compared with PHBV, PHBV/CSH scaffolds (P < 0.01). (7) These results confirm that compared with other scaffolds, PHBV/CSH/CS exhibits better cell adhesion, osteogenesis, antibacterial properties and drug loading potential.

Key words: 3D printing, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), calcium sulfate hemihydrate, chitosan, hydrogel, biocompatibility, antibacterial activity

CLC Number:

Ye Xiangling, Xia Yuanjun, Wang Boqun, Kang Zhengyang, Wu Bin. Function on 3D printing poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/calcium sulfate hemihydrate scaffold integrated chitosan hydrogel coating[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(10): 1574-1581.

Figures/Tables 10

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