Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (3): 329-334.doi: 10.12307/2022.901

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Evaluation of immunotoxicity of the absorbable macroporous polysaccharides composite hemostatic material

Wu Lihao1, Shao Anliang2, Xu Lin1, Ren Kang1, Wang Hongjian1, Chen Liang2, Xu Ling1   

  1. 1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, Fujian Province, China; 2National Institutes for Food and Drug Control, Beijing 102629, China
  • Received:2021-03-27 Accepted:2021-05-09 Online:2023-01-28 Published:2022-05-20
  • Contact: Xu Ling, DE, Professor, Doctoral supervisor, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, Fujian Province, China Chen Liang, MD, Researcher, National Institutes for Food and Drug Control, Beijing 102629, China
  • About author:Wu Lihao, Master candidate, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, Fujian Province, China Shao Anliang, MD, National Institutes for Food and Drug Control, Beijing 102629, China Wu Lihao and Shao Anliang contributed equally to this article.
  • Supported by:
    Fujian Marine Economy Development Subsidy Fund Project, No. FJHJF-L-2019-4 (to XL); Xiamen Ocean and Fisheries Development Special Fund Project, No. 18CYY007HJ04 (to XL); The Fifth Round of Fujian Health Education Joint Key Project, No. 2019-WJ-36 (to XL)

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Abstract: BACKGROUND: Our previous studies manifested that the absorbable macroporous polysaccharides composite hemostatic material has a good hemostatic effect, but its immunotoxicity has not been further studied.
OBJECTIVE: To evaluate the immunotoxicity of absorbable macroporous polysaccharides composite hemostatic materials in mice. 
METHODS: The 48 BALB/c mice were randomly divided into four groups (n=12). No material was implanted in the subcutaneous capsule in the sham-operated group. Mice in the experimental 1 group were implanted with absorbable macroporous polysaccharides composite hemostatic materials without purification in the subcutaneous capsule. Mice in the experimental 2 group were implanted with absorbable macroporous polysaccharides composite hemostatic materials with purification in the subcutaneous capsule. Mice in the control group were implanted with compound microporous polysaccharide hemostatic powder. At 14 and 28 days after implantation, mass and organ/body weight ratio of the heart, liver, spleen, lungs, kidney, adrenal gland, thymus, testis and epididymis, serum IgG and IgM concentraions, proportion of B cells and T cells in spleen lymphocytes, and pathological changes of spleen and thymus were analyzed. 
RESULTS AND CONCLUSION: (1) During the experiment, the mice with implanted material were in good condition without obvious poisoning reaction. (2) On day 14 after implantation, the heart weight of the experimental 1 group was higher than that in the sham-operated group (P < 0.05). Lung organ weight and coefficient were lower in the control group than those in the sham-operated group (P < 0.05). Kidney organ weight and coefficient were higher in the experimental 2 group than those in the sham-operated group (P < 0.05). At 14 and 28 days after implantation, other organ weight and coefficient were not significantly different between the experimental 1, 2, and control groups and sham-operated group (P > 0.05). (3) IgM concentration was lower in the experimental 2 group than that in the sham-operated group at 14 days (P < 0.05). IgG concentration was higher in the experimental 1 group than that in the sham-operated group at 28 days (P < 0.05). (4) At 14 and 28 days after implantation, the proportion of B cells and T cells in spleen lymphocytes was not significantly different between the experimental 1 and 2 groups and control group (P > 0.05). (5) At 14 and 28 days, hematoxylin-eosin staining showed slight chronic inflammation in the experimental 1, 2, and control groups. The degree of inflammatory reaction was experimental 2 group < experimental 1 group < control group. (6) These findings verify that absorbable macroporous polysaccharides composite hemostatic material has a low immunotoxicity. 

Key words: hemostasis, absorbable, hemostatic material, polysaccharide, carboxymethyl chitosan, immunotoxicity, degradation in vivo

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