Effect of tea polyphenols on proliferation and differentiation of osteoblasts stimulated by lipopolysaccharide

doi:10.12307/2023.685

Abstract

Abstract: BACKGROUND: Studies have shown that tea polyphenols can play anti-inflammatory and antioxidant effects in the investigation of periodontitis and other diseases.
OBJECTIVE: To investigate the effects of tea polyphenols on the proliferation, differentiation and oxidative stress of mouse osteoblasts induced by lipopolysaccharide.
METHODS: The mouse osteoblasts MC3T3-E1 were cultured in vitro, and the MC3T3-E1 cells were randomly divided into four groups, namely the control group (conventional culture), the lipopolysaccharide group (10 μg/mL lipopolysaccharide), the tea polyphenols group (1 μg/mL tea polyphenols), and the combination group (10 μg/mL lipopolysaccharide + 1 μg/mL tea polyphenols). The MTT assay was used to detect the cell proliferation. The alkaline phosphatase activity was detected by alkaline phosphatase kit. The alkaline phosphatase staining degree was detected by alkaline phosphatase azo coupling method. The calcium deposition amount of the cells was detected by calcium detection kit. The superoxide content of cells was detected by superoxide kit. The malondialdehyde content of cells was detected by malondialdehyde kit.
RESULTS AND CONCLUSION: (1) Compared with the control group, the proliferation activity, alkaline phosphatase activity and calcium deposition of osteoblasts were significantly decreased in the lipopolysaccharide group (P < 0.05); the staining degree of alkaline phosphatase was significantly decreased (P < 0.01); and the contents of superoxide and malondialdehyde were significantly increased (P < 0.05). The proliferation activity, alkaline phosphatase activity and calcium deposition of osteocytes in the tea polyphenols group were significantly increased (P < 0.05); the staining degree of alkaline phosphatase was significantly increased (P < 0.01); and the contents of superoxide and malondialdehyde were significantly decreased (P < 0.05). (2) Compared with the lipopolysaccharide group, the proliferation activity and calcium deposition of bone cells in the combination group were significantly increased (P < 0.05); the staining degree of alkaline phosphatase was significantly increased (P < 0.001); and the contents of superoxide and malondialdehyde were significantly decreased (P < 0.05). (3) The results showed that tea polyphenols can promote the proliferation and differentiation of osteoblasts, reduce the expression levels of oxidative stress factors, and reverse the adverse effects of lipopolysaccharides on osteoblasts.

Key words: osteoblast, lipopolysaccharide, tea polyphenols, proliferation, osteogenic differentiation, oxidative stress

CLC Number:

Qin Yixiong, Yuan Zijian, Xie Shanzhou, Zhu Yingwen, Chen Minghui, Han Biao, Guo Yong. Effect of tea polyphenols on proliferation and differentiation of osteoblasts stimulated by lipopolysaccharide[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(35): 5665-5669.

Figures/Tables 6

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