Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (34): 5498-5503.doi: 10.12307/2022.462
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Sun Xirao, Bao Jiaxin, Wang Chengyue
Received:
2021-05-07
Accepted:
2021-07-10
Online:
2022-12-08
Published:
2022-04-15
Contact:
Wang Chengyue, Chief physician, Second Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, China
About author:
Sun Xirao, Master, Attending physician, Second Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, China
Supported by:
CLC Number:
Sun Xirao, Bao Jiaxin, Wang Chengyue. Construction of chitosan/mineralized collagen porous scaffold, osteogenic differentiation in vitro and biocompatibility[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(34): 5498-5503.
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3组多孔支架中含有典型的壳聚糖的X射线衍射峰,在10°和20°左右出现宽峰,峰的强度随着壳聚糖含量的减少而降低。在傅里叶红外光谱中,所有样品在3 440 cm-1处的吸收峰归属于羟基和氨基,来自壳聚糖和Ⅰ型胶原;对于单纯的纯壳聚糖多孔支架,在3 293 cm-1处的振动被分配为羟基拉伸,该振动可能与氨基拉伸在90°时重叠;与矿化胶原粉体相比,所有CS/nHAC材料在2 930,2 870 cm-1处都出现了吸收峰,这是由于C-H极性共价键在壳聚糖中的振动吸收峰出现了伸缩;所有样品的特征峰大约为1 657 cm-1,与存在于壳聚糖和Ⅰ型胶原中的羰基(amide Ⅰ)有反应;除单纯壳聚糖多孔支架外,其他样品在1 033,604,565 cm-1处出现吸收带,与PO43-离子对应,表明存在羟基磷灰石,与X射线衍射图谱结果一致。上述结果表明,矿化胶原和壳聚糖已成功地结合[8-9]。 2.2 多孔支架材料表面的细胞黏附 骨组织工程支架必须对细胞无毒副作用,实验采用直接共培养实验分析不同材料与细胞直接接触时的黏附状态。 图3为在不同多孔支架材料上培养1 d的小鼠成骨前体细胞扫描电镜观察结果。单纯壳聚糖支架表面的细胞拉伸面积增大,细胞完全伸展;2CS/1nHAC和1CS/1nHAC多孔支架表面的细胞密度高于单纯壳聚糖支架,细胞丝足相互连接,材料表面的小鼠成骨前体细胞具有良好的黏附性,并伸出大量的伪足,细胞在材料表面的孔隙中相互连接并向内生长。此外,壳聚糖和矿化胶原作为生物相容性优良的材料,也为细胞的黏附和生长提供了良好的周边环境。"
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