Chitosan/alginate composite scaffold combined with hawthorn leaf flavonoids for spinal cord injury repair

doi:10.12307/2022.299

Abstract

Abstract: BACKGROUND: Studies have shown that hawthorn leaf total flavonoids have a certain therapeutic effect on spinal cord injury, but the method of administration is limited to intraperitoneal injection, and there are rare reports of direct application to the site of spinal cord injury.
OBJECTIVE: To compare the effects of chitosan alginate composite scaffold loaded with hawthorn leaf flavonoids sustained-release microspheres and intraperitoneal injection of hawthorn leaf flavonoids combined with chitosan alginate composite scaffold in the repair of spinal cord injury.
METHODS: Chitosan alginate composite scaffolds, hawthorn leaf flavonoids sustained-release microspheres and chitosan alginate composite scaffolds loaded with hawthorn leaf total flavonoids sustained-release microspheres were prepared. Forty Sprague-Dawley rats were used to establish a model of complete spinal cord injury and were randomly divided into four groups. Injury control group received intraperitoneal injection of saline after surgery. Rats in the stent group were implanted with a chitosan alginate composite stent, and injected intraperitoneally with physiological saline after the operation. Rats in the stent + drug injection group were implanted with chitosan alginate composite stent, and injected intraperitoneally with 20 mg/(kg·d) hawthorn leaf total flavonoids after surgery. Rats in the drug loaded stent group were implanted with chitosan alginate composite stent loaded with sustained-release microspheres, and injected intraperitoneally with physiological saline after the operation. BBB score and inclined plate test were used to evaluate the motor function of rats within 8 weeks after operation. The pathological morphology and western blot assay of spinal cord tissue were observed at 8 weeks after operation.
RESULTS AND CONCLUSION: (1) Since 2 weeks after operation, BBB score and inclined plate test angle of three groups of stent implantation were higher than those of injury control group (P < 0.05), and those of stent + drug injection group and drug loaded stent group were higher than those of stent group (P < 0.05), and that of drug loaded stent group was higher than that of stent + drug injection group (P < 0.05). (2) Hematoxylin-eosin staining showed that in the injury control group, no spinal cord tissue was found in the injured area, and only thin filamentous connection was found. The stent implanted in the other three groups was closely connected with the injured spinal cord end, and tissue and cells grew into the scaffold material. There were more tissues in the stent + drug injection group and drug loaded stent group. (3) Western blot assay demonstrated that the expression of glial fibrillary acidic protein in three groups of stent implantation was lower than that in injury control group (P < 0.05). The expression levels of neurofilament protein 200, myelin basic protein, and growth-associated protein 43 were higher in the three groups of stent implantation than those in the injury control group (P < 0.05). The expression of glial fibrillary acidic protein in drug loaded stent group was lower than that in stent group and stent + drug injection group (P < 0.05), and that in stent + drug injection group was lower than that in stent group (P < 0.05). The expression levels of neurofilament protein 200, myelin basic protein, and growth-associated protein 43 were higher in drug loaded stent group than that in stent group and stent + drug injection group (P < 0.05). The expression levels of neurofilament protein 200, myelin basic protein, and growth-associated protein 43 were higher in stent + drug injection group than that in stent group (P < 0.05). (4) The results have showed that intraperitoneal injection of total flavonoids from hawthorn leaves or local application of sustained-release microspheres combined with chitosan alginate composite scaffolds can promote the repair of spinal cord injury, and the effect of local application of sustained-release microspheres is better.

Key words: chitosan, alginate, hawthorn leaf flavonoids, spinal cord injury, composite stent, sustained release microspheres

CLC Number:

Liu Ming, Wang Kai. Chitosan/alginate composite scaffold combined with hawthorn leaf flavonoids for spinal cord injury repair[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(28): 4466-4471.

Figures/Tables 6

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