Osteogenic ability of adipose stem cells in diabetic osteoporosis mice

doi:10.12307/2022.135

Abstract

Abstract: BACKGROUND: Long noncoding RNA (LncRNA)-DANCR can regulate multiple biological processes, including bone metabolism. Our gene microarray results showed that LncRNA-DANCR was significantly overexpressed in diabetic osteoporosis mice.
OBJECTIVE: To investigate the effect of LncRNA-DANCR on the osteogenic ability of adipose stem cells in diabetic osteoporosis through Wnt/β-catenin signaling pathway.
METHODS: Diabetic osteoporosis adipose-derived stem cells and control adipose-derived stem cells were isolated and cultured. After 3 days of osteogenic induction, real-time fluorescence quantitative PCR and western blot assay were used to examine the expression of LncRNA-DANCR, Wnt signaling molecules β-catenin and osteogenic transcription factors (RUNX2 and OPN). Diabetic osteoporosis adipose-derived stem cells transfected with lncRNA-DANCR-specific siRNA were designed. After 3 days of osteogenic induction, real-time fluorescence quantitative PCR, western blot assay and alkaline phosphatase staining were used to examine the expression of Wnt signaling molecules β-catenin, osteogenic transcription factors RUNX2 and OPN and osteogenic ability changes.
RESULTS AND CONCLUSION: (1) After 3 days of osteogenic induction, the expression level of LncRNA-DANCR in diabetic osteoporosis adipose-derived stem cells group was significantly higher than that in control group. The expression levels of Wnt signaling molecule β-catenin and osteogenic transcription factors RUNX2 and OPN were significantly lower than those in control adipose-derived stem cells. (2) After 3 days of osteogenic induction, β-catenin and RUNX2 and OPN were highly expressed in the siRNA silent group, and the ability of bone formation was enhanced. (3) The results confirmed that LncRNA-DANCR decreased the osteogenic capacity of diabetic osteoporosis adipose-derived stem cells by inhibiting the Wnt/β-catenin signaling pathway. Silencing of LncRNA-DANCR can restore the reduced osteogenic capacity.

Key words: long noncoding RNA, Wnt signaling pathway, diabetes, osteoporosis, osteogenic differentiation, bone repair

CLC Number:

Gao Yujin, Peng Shuanglin, Ma Zhichao, Lu Shi, Cao Huayue, Wang Lang, Xiao Jingang. Osteogenic ability of adipose stem cells in diabetic osteoporosis mice[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(7): 999-1004.

Figures/Tables 9

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