A hydrogel based on acellular decalcified bone matrix/erythropoietin promotes osteogenesis and angiogenesis

doi:10.12307/2021.057

Abstract

Abstract:

BACKGROUND: In recent years, acellular decalcified bone matrix (ADBM) exhibits tremendous potential and application prospects in bone defect repair due to its favorable biocompatibility and biomechanics. Erythropoietin (EPO) can promote the formation of local blood vessels and thus indirectly regulate bone repair owing to crucial role in angiogenesis of bone repair.

OBJECTIVE: To investigate the effects of acellular decalcified bone matrix/erythropoietin (ADBM-EPO) hydrogel on osteogenesis and angiogenesis.
METHODS: ADBM hydrogel and ADBM-EPO hydrogel were constructed. Human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells were respectively seeded on the two hydrogels, and the conventionally cultured cells were used as the control group. The effects on the migration ability of two kinds of cells were detected through cell transmembrane invasion and cell scratch migration experiments. The effects of the two hydrogels on osteogenic differentiation of bone marrow mesenchymal stem cells were determined by alkaline phosphatase and alizarin red staining.
RESULTS AND CONCLUSION: (1) Compared with the control group, ADBM hydrogel and ADBM-EPO hydrogel could promote the migration of bone marrow mesenchymal stem cells or umbilical vein endothelial cells. Moreover, the ADBM-EPO hydrogel had better migration ability than that of ADBM hydrogel. (2) Compared with the control group, both ADBM and ADBM-EPO hydrogels could reduce the scratch distance of human bone marrow mesenchymal stem cells or human umbilical vein endothelial cells. Of them, ADBM-EPO hydrogel was more effective than ADBM hydrogel. (3) Compared with the control group, both ADBM and ADBM-EPO hydrogels could increase the alkaline phosphatase activity and the number of calcium nodules in bone marrow mesenchymal stem cells. Moreover, the ADBM-EPO hydrogel had better ability than that of ADBM hydrogel. (4) The above experimental results showed that the ADBM-EPO hydrogel had a bidirectional role in promoting osteogenesis and angiogenesis.

Key words: materials, hydrogel, decellularization, decalcified bone matrix, erythropoietin, cell migration, osteogenic differentiation, stem cells, endothelial cells

CLC Number:

Zhou Jianwei, Zhou Jing, Li Mao, Chi Cheng, Wang Fei. A hydrogel based on acellular decalcified bone matrix/erythropoietin promotes osteogenesis and angiogenesis[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(28): 4454-4459.

Figures/Tables 4

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