A novel compound biological antibacterial dressing: Antimicrobial effectiveness, moisture absorption capacity and cytotoxicity

doi:10.3969/j.issn.2095-4344.2013.16.008

Abstract

Abstract:

BACKGROUND: Bacterial infection is one of the main factors affecting wound healing. Wound exudate containing large amount of inflammatory cytokines, proteases and free radicals will delay wound healing. Research and development of new compound biological antimicrobial dressings have important implications for treating surgery wound infection, which is the inevitable trend of the development of wound dressings.
OBJECTIVE: To observe the antibacterial activity, moisture absorption capacity and cytotoxicity of calcium alginate dressing containing nanocrystalline silver.
METHODS: Silver nanoparticles were added into calcium alginate to prepare new compound biological antimicrobial dressings. The antibacterial activity, moisture absorption capacity and cytotoxicity of calcium alginate dressing containing nanocrystalline silver were detected using the plate count method, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, electron microscope experiment, compared with silver ion calcium alginate dressing and calcium alginate dressing, in the hope of showing the strong antimicrobial activity and low cytotoxicity of new compound biological antimicrobial dressings.
RESULTS AND CONCLUSION: The new compound biological nano-silver antimicrobial dressing, compared with the silver ion calcium alginate dressing and calcium alginate dressing, had stronger bacteriostasis to Staphylococcus aureus and Pseudomonas aeruginosa (P < 0.01), and exhibited lower cytotoxicity (P < 0.01). There occurred no significant differences in moisture absorption capacity among the three groups (P > 0.05). It is proved that the new compound biological nano-silver antimicrobial dressing has stronger antibacterial activity and lower cytotoxicity.

Key words: biomaterials, biomaterials and controlled drug release, novel biological dressings, nano-silver, alginate fiber, antibacterial, moisture absorption, cytotoxicity, scanning electron microscope

CLC Number:

R318

Lin Xiao-hua, Li Zhi-chao, Yu Jin-long, Xiao Zhong, Zou Zhao-wei, Huang Zong-hai, Wang Xiao-dong, Mo Xiao-hui. A novel compound biological antibacterial dressing: Antimicrobial effectiveness, moisture absorption capacity and cytotoxicity[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2905-2912.

Figures/Tables 6

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