Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (22): 3540-3544.doi: 10.3969/j.issn.2095-4344.3234

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Hydrophilicity and cell adhesion of hydroxyapatite bioceramics after the coating of polydopamine

He Lin1, Wu Xi2, He Song2, Yang Sen1   

  1. 1Dental Center of Suining Central Hospital, Suining 629000, Sichuan Province, China; 2Stomatological Hospital Affiliated to Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2020-07-10 Revised:2020-07-11 Accepted:2020-08-11 Online:2021-08-08 Published:2021-01-20
  • Contact: Yang Sen, MD, Master’s supervisor, Associate chief physician, Dental Center of Suining Central Hospital, Suining 629000, Sichuan Province, China
  • About author:He Lin, Master, Physician, Dental Center of Suining Central Hospital, Suining 629000, Sichuan Province, China
  • Supported by:
    the Clinical Research Fund of Stomatology in Western China of Chinese Stomatological Association, No. CSA-W-2016-01 (to YS); the Sichuan Huimin Oral Health Promotion Center, No. SCHMKQ-KF-002 (to YS)

Abstract: BACKGROUND: Bone graft materials are the key points and difficulties in current research. Hydroxyapatite has good biocompatibility and degradation. However, the mechanical properties of synthetic hydroxyapatite are poor and lack of osteoinductive properties. It is more close to clinical application to combine the more easily obtained apical papilla stem cells with hydroxyapatite bioceramics, which is widely used in clinical bone repair.           
OBJECTIVE: To observe the physicochemical properties and cytotoxicity of hydroxyapatite bioceramics coated with polydopamine.           
METHODS: Polydopamine-hydroxyapatite bioceramics were prepared by placing hydroxyapatite bioceramics in dopamine Tris buffer solution. Uncoated hydroxyapatite bioceramics were used as control. The average pore diameter, porosity and contact angle of water droplets of polydopamine coated hydroxyapatite bioceramics and uncoated hydroxyapatite bioceramics were measured. The third generation of human apical papilla stem cells was inoculated on the two kinds of ceramic surfaces respectively. After 15 minutes of culture, the cell adhesion was observed under inverted microscope and laser confocal microscope. After 1, 3, 5 and 7 days of culture, the cytotoxicity was detected by CCK-8 assay.
RESULTS AND CONCLUSION: (1) There was no significant difference in the average pore size and porosity of hydroxyapatite bioceramics before and after coating (P > 0.05). The particles on the surface of polydopamine coated hydroxyapatite bioceramics were larger and the surface of polydopamine coated hydroxyapatite bioceramics was more flat. (2) The contact angle of polydopamine coated hydroxyapatite bioceramics was lower than that of uncoated hydroxyapatite bioceramics (P < 0.001). (3) Inverted microscope showed that human apical papilla stem cells were scattered around the uncoated cell slides, but not around the coated cell slides; and uniformly adhered human root tip papilla stem cells were observed on the surface of polydopamine coated cell slides. Laser confocal microscopy showed that compared with uncoated cell slides, the volume of human apical papilla stem cells on the surface of polydopamine coated cell climbing tablets was larger, more fully extended, and cell antennae were more obvious. (4) The relative proliferation rates of human apical papilla stem cells cultured at different time points on the two scaffolds were above 75%, and the cytotoxicities were 0-1 grade. (5) The results showed that compared with uncoated hydroxyapatite bioceramics, polydopamine coated hydroxyapatite bioceramics had better hydrophilicity and cell adhesion.

Key words: materials, oral cavity, polydopamine, hydroxyapatite, bioceramics, human apical papilla stem cells, cytotoxicity, hydrophilicity, adhesion

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