Table of Content

16 April 2013, Volume 17 Issue 16 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Autologous periodontal ligament stem cells combined with composites repair periodontal bone defects in miniature pigs Yang Si-hui, Zhong Liang-jun, Zhang Peng-tao,Zhang Yuan,Zhang Yuan-ming,Ma Lu-ping 2013, 17 (16):  2851-2858.  doi: 10.3969/j.issn.2095-4344.2013.16.001 Abstract ( 426 )   PDF (1048KB) ( 791 )   Save BACKGROUND: Studies have proved that autologous periodontal ligament stem cells combined with scaffold materials can achieve better effect on the repair of periodontal bone defects. OBJECTIVE: To observe the effect of miniature pig autologous periodontal ligament stem cells combined with hydroxyapatite bioceramic composites in the repair of category Ⅱ periodontal bone defects. METHODS: Six Guizhou miniature pigs were included, and were used to establish the miniature pig models of upper and lower jaw category Ⅱ periodontal bone defects. The bone defects were located between the third premolar and fourth premolar, and the near root of fourth premolar was exposed. The defects in the experimental group were repaired with periodontal ligament stem cells obtained from the autologous maxillary and mandibular canine and combined with hydroxyapatite bioceramic; the defects in the control group were repaired with hydroxyapatite bioceramic simplely; and the model group did not repaired. The defects in each group were covered with oral biofilm. At 12 weeks after modeling, the defect periodontal tissues were obtained from each group, and then the osteogenesis healing of the periodontal bone tissue was observed through clinical observation, heal spiral CT scanning, three-dimensional reconstruction and hematoxylin-eosin staining. RESULTS AND CONCLUSION: Clinical observation showed that healing of periodontal defects in the experimental group was the best. Head spiral CT scanning showed that there was no significant difference of bone mineral density between bone defect area and surrounding normal alveolar bone, and the defects in the control group and the model group were still clear visible in the incomplete healing state. Hematoxylineosin staining showed that the defect area in the experimental group was filled with newborn alveolar bone completely, and the normal calcified bone structure was established; in the control group, the defect area was filled with newborn alveolar bone incompletely with low calcification degree; a large number of collagen fibers could be seen in the model group, and the defect area was not filled and no new bone formed. It is visible that periodontal ligament stem cells combined with guided tissue regeneration can effectively promote healing of category II periodontal bone defects and periodontal regeneration in miniature pigs, thus repairing periodontal bone defects. Figures and Tables | References | Related Articles | Metrics

Select

Bone marrow mesenchymal stem cells seeded into an allogeneic scaffold repair critical-sized iliac defects in sheep Yang Nan, He Hui-yu, Hu Yang, Yang Chuan-bo 2013, 17 (16):  2859-2868.  doi: 10.3969/j.issn.2095-4344.2013.16.002 Abstract ( 318 )   PDF (611KB) ( 640 )   Save BACKGROUND: Nowadays, the research regarding tissue engineering bone materials and construction methods continues to heat up, and how to repair large bone defects is still difficult in the clinic. OBJECTIVE: To compare the effects of tissue engineering bones with allogeneic scaffold material, β-tricalcium phosphate material and basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cells on the repairing of critical-sized iliac defects in sheep. METHODS: The sheep bone marrow mesenchymal stem cells were in vitro induced and cultured to the third generation, and then the basic fibroblast growth factor lentiviral vector was constructed. The allogeneic scaffold materials with transfected and untransfected bone marrow mesenchymal stem cells, simple allogeneic scaffold materials and β-tricalcium phosphate materials were implanted into the sheep model of critical-sized iliac defects   (15 mm×10 mm×10 mm). Imaging, histology, and scanning electron microscopy observations were performed at 4, 8 and 12 weeks after repairing. RESULTS AND CONCLUSION: The repairing effect of allogeneic scaffold materials with basic fibroblast growth factor lentiviral vector transfected bone marrow mesenchymal stem cells on sheep critical-sized iliac defects was better than that of untransfected allogeneic scaffold materials, simple allogeneic scaffold materials and β-tricalcium phosphate materials; the effect of untransfected allogeneic scaffold materials was better than that of simple allogeneic scaffold materials and β-tricalcium phosphate materials; the degradation rate of simple allogeneic scaffold materials was higher than that of β-tricalcium phosphate materials. The tissue engineering bone constructed using allogeneic scaffold materials with basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cells can meet the requirement of bone repairing, and it can guide the new bone formation with good biocompatibility and cannot interfere with bone tissue reconstruction and modeling. Figures and Tables | References | Related Articles | Metrics

Select

Allogenic deproteinized bone combined with fibrin glue constructs tissue engineered bone in vivo Miao Xu-dong, Pei Guo-xian, Yan Qiao-sheng, Jia Jing, Xu You 2013, 17 (16):  2869-2873.  doi: 10.3969/j.issn.2095-4344.2013.16.003 Abstract ( 344 )   PDF (379KB) ( 463 )   Save BACKGROUND: Some experiments have demonstrated that allogenic deproteinized bone combined fibrin glue is more suitable for seed cells growth and proliferation in vitro. OBJECTIVE: To observe the difference between allogenic deproteinized bone combined fibrin glue and allogenic deproteinized bone alone as scaffold materials for bone tissue engineering.  METHODS: Twenty-eight New Zealand white rabbits were randomly divided into three groups: allogenic deproteinized bone combined fibrin glue composited with mesenchymal stem cells was implanted in experiment group, allogenic deproteinized bone composited with mesenchymal stem cells implanted in control group, and allogenic deproteinized bone alone implanted in blank group. RESULTS AND CONCLUSION: With the duration of implantation, the alkaline phosphatase activity of eachgroup was increased gradually. The experiment group was higher than control group and blank group in the alkaline phosphatase activity (P < 0.01). New bone tissue formed in both the experiment group and control group at 4 and 8 weeks after implantation, and there were more new bone tissues formed in the experiment group (P < 0.01). In the blank group, there was no new bone formed. Allogenic deproteinized bone combined fibrin glue is more suitable for mesenchymal stem cells than allogenic deproteinized bone alone, and it can be used as a scaffold for bone tissue engineering. Figures and Tables | References | Related Articles | Metrics

Select

Safety of true bone ceramics Liu BY, Guo MF, Xing YX, Ma CG 2013, 17 (16):  2874-2882.  doi: 10.3969/j.issn.2095-4344.2013.16.004 Abstract ( 381 )   PDF (390KB) ( 607 )   Save BACKGROUND: True bone ceramic treated with high temperature has continuous microporous structure, good biological compatibility and degradation, which are analogous to nature bone. OBJECTIVE: To observe the biocompatibility, cellular compatibility and acute toxicity of implanted composite made from periosteal-derived osteoblasts and sintered bovine bone (true bone ceramics). METHODS: Experiment of cellular compatibility: bovine true bone ceramics were compound cultured with induced passage 3 bone marrow mesenchymal stem cells of Wistar rats. Hemolysis test: the true bone ceramic water extract, normal saline and double-distilled water were added into rabbit blood. Blood coagulation test: the true bone ceramics were added into rabbit blood plasma. Systemic acute toxicity test: true bone ceramic water extract and normal saline were injected into the Kunming mice via the tail vein respectively. Micronucleus test: true bone ceramic water extract, normal saline and cyclophosphamide were given into the mice through intraperitoneal injection respectively. Local irritation reaction: true bone ceramic water extract and normal saline were injected at the two sides of the spine respectively. Pyrogen test: true bone ceramic water extract was injected via the ear vein. The subcutaneous implantation test: the true bone ceramic materials were implanted dorsal subcutaneously. RESULTS AND CONCLUSION: True bone ceramics had no cell toxicity, but had good cellular and blood compatibility; had no stimulation to muscle and skin; had no toxicity on heart, liver and kidney; had no stimulation to the surrounding tissues after subcutaneous implantation, and part of the materials were degraded and replaced by newly developed fibrous tissue; had no pyrogenic action and had no influence on coagulation; had no inhibition and toxic effect on bone marrow cells. Figures and Tables | References | Related Articles | Metrics

Select

Subcutaneous osteogenesis by bone marrow stromal cells combined with sintered bone Yang Chuan-bo, He Hui-yu, Cui Jie, Ma Wen-yuan, Yang Nan 2013, 17 (16):  2883-2890.  doi: 10.3969/j.issn.2095-4344.2013.16.005 Abstract ( 382 )   PDF (458KB) ( 506 )   Save BACKGROUND: It has proved that the bone marrow stromal cells and sintered bone support material combination can form tissue-engineered bone, but relevant studies on its biocompatibility in animals and subcutaneous osteogenesis ability are less reported in China. OBJECTIVE: To observe the osteogenesis capacity of marrow stromal stem cells combined with sintered bone implanted into Balb/c nude mouse back subcutaneously and to explore the feasibility of sintered bone as a scaffold for tissue-engineered bone. METHODS: Sintered bone scaffold materials were prepared using defatted and deproteinized processing and high-temperature calcinations. Then the sintered bone was combined with passage 3 sheep bone marrow stromal stem cells cultured using density gradient centrifugation method to be implanted subcutaneously into the back of BALB/c nude mice. Simple sintered bone that was subcutaneously implanted into the contralateral back of BALB/c nude mice served as control group. RESULTS AND CONCLUSION: After calcinations, sintered cancellous bone block colored chalks and the surface displayed a honeycomb porous structure, maintaining the porous structure of the natural cancellous bone. Bone trabecular structure was of integrity, and pores were mutually interconnected. After bone marrow stromal stem cells were inoculated to the calcined bone, there were a lot of cells adherent to the scaffold within 24 hours, and a large amount of extracellular matrix at 7 days. The boundary between cells and extracellular matrix was unclear, and the cells could grow and proliferate well on the scaffold, suggesting that the cell viability was not influenced by the scaffold. Four weeks after implantation, a few of fragments were visible at the edge of sintered bone in the two groups. Bone cells could be seen around the pores of sintered bone in the combination group, while fibrous connective tissue enveloped the sintered bone in the control group. Eight weeks later, the sintered bone partially degraded into osteoid sheets surrounded by fibroblasts that arranged closely and was diverse in shape. In the combination group, osteoblasts arranged in line on the surface of sintered bone and infiltrated lymphocytes were scattered among the pores. In the control group, a great amount of connective tissues grew into the pores, but there was no osteogenesis. These findings indicate that the sintered cancellous bone block pose better biocompatibility and biological safety, which can be a better scaffold for bone marrow stromal stem cells. The composite of bone marrow stromal stem cells and sintered bone has a good ability to induce new bone formation and the sintered bone can be used as scaffold materials for tissue engineering repair of bone defects. Figures and Tables | References | Related Articles | Metrics

Select

Pharmacodynamics effect of nerve growth factor-loaded nanoparticles to induce PC12 cells Bao Guo-qing, Long Da-hong, Chen Yan, Liu Fei-fei, Zhang Jun-du 2013, 17 (16):  2891-2898.  doi: 10.3969/j.issn.2095-4344.2013.16.006 Abstract ( 555 )   PDF (484KB) ( 536 )   Save BACKGROUND: It is found that the surface-modified polymer nanoparticles can pass through the blood-brain barrier, and improve the drug effects on the central nervous system diseases. OBJECTIVE: To prepare nerve growth factor-loaded high encapsulation efficiency nanoparticles with a iodegradable material, polyethylene glycol-polylactic acid glycolic acid copolymer, and to explore its effect on PC12 cells. METHODS: Bovine serum albumin-loaded polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles were prepared using complex emulsion solvent diffusion method. The production process was screened by univariate analysis and orthogonal design method. The nanoparticle morphology was observed under scanning electron microscopy, and the average particle diameter and the dispersion index were measured by nanoparticle analyzer. The nanoparticle encapsulation efficiency and drug loading were analyzed by BCA method. Nanoparticle release in vitro was also investigated. After optimization of the preparation program, nerve growth factor-loaded polymer nanoparticles were prepared and applied to PC12 cells. The cells were then observed by inverted fluorescence microscope, and the induction effects, toxicity and slow-release effect of polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles loaded with nerve growth factor were evaluated. RESULTS AND CONCLUSION: Bovine serum albumin-loaded polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles were prepared by optimal method. The nanoparticles were spherical, and uniform in size, with an average particle size of (258.9±5.73) nm, and the encapsulation efficiency was (80.56±2.23)%. When the dosage within the aqueous phase was 10 mg, the drug loading was (4.24±0.12)%. The in vitro release of nanoparticles was in accordance with the Higuchi equation of. Release of the nanoparticles could be divided into two stages: initial burst release stage and late sustained release. The total cumulative release amount of bovine serum albumin and nerve growth factor in 0-56 days was 76.61% and 62.34%, respectively. The PC12 cells could be induced to differentiation by polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles loaded with nerve growth factor, and the nanoparticles exhibited good release properties and no toxic effects. It was showed that nerve growth factor-loaded polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles prepared by the optimal method had excellent physicochemical properties, good release properties in vitro and no toxic effects. Figures and Tables | References | Related Articles | Metrics

Select

Inorganic nanoparticles-embedded bioactive carbon nanofibers: Preparation and performance evaluation Chi Ming-chao, Sui Gang, Yang Qing, Duan Shun, Shi Yu-zhou, Cai Qing, Yang Xiao-ping 2013, 17 (16):  2899-2904.  doi: 10.3969/j.issn.2095-4344.2013.16.007 Abstract ( 376 )   PDF (1588KB) ( 547 )   Save BACKGROUND: It has been identified that the introduction of β-tricalcium phosphate nanoparticles, which have good osteogenetic activity, into bio-inert carbon nanofibers can significantly improve the biological activity of carbon nanofibers. Furthermore, it has also been reported that bivalent ions doped β-tricalcium phosphate compounds can promote the new bone formation. OBJECTIVE: To investigate the effect of incorporation of Zn2+, Mg2+ on the fiber morphology and the osteogenetic activity of β-tricalcium phosphate@carbon nanofibers. METHODS: To prepare Zn2+/Mg2+ doped β-tricalcium phosphate@carbon nanofibers composite nanofibers, electospinning of polyacrylonitrile solution was combined with CaP sol-gel solution by using triethyl phosphate, calcium nitrate, zinc nitrate and/or magnesium nitrate as precursors. The carbon nanofiber composite was obtained by pre-oxidizing and carbonizing the as-electrospun nanofibers. By in vitro co-culture with mouse osteoblast-like cells (MC3T3-E1), the effect of Zn2+/Mg2+ doping into β-tricalcium phosphate@carbon nanofibers on cell behaviors were evaluated via comparison of cell adhesion efficiency, proliferation and morphology. RESULTS AND CONCLUSION: The obtained Zn2+/Mg2+ doped β-tricalcium phosphate@carbon nanofibers demonstrated uniform and smooth fiber surface with no bead-in-string structure. Inorganic particles in nanoscale could be seen clearly on the fiber surface and throughout the fiber. Determined by element analysis, it was revealed that the composite fibers were mainly composed of carbon, and the calcium element. The mapping of Mg and Zn showed their distribution was even and their amounts were coincident to feed ratios. Compared to non-doping β-tricalcium phosphate@carbon nanofibers, MC3T3-E1 showed enhanced biological behaviors on Zn2+ or Mg2+ doped β-tricalcium phosphate@carbon nanofibers matrixes, like cell adhesion, spreading, and proliferation being promoted. The results suggested that ions doped β-tricalcium phosphate@carbon nanofibers with higher cytocompatibility and bioactivity might be a kind of attractive substrate for bone regeneration. Figures and Tables | References | Related Articles | Metrics

Select

A novel compound biological antibacterial dressing: Antimicrobial effectiveness, moisture absorption capacity and cytotoxicity Lin Xiao-hua, Li Zhi-chao, Yu Jin-long, Xiao Zhong, Zou Zhao-wei, Huang Zong-hai, Wang Xiao-dong, Mo Xiao-hui 2013, 17 (16):  2905-2912.  doi: 10.3969/j.issn.2095-4344.2013.16.008 Abstract ( 788 )   PDF (503KB) ( 1017 )   Save BACKGROUND: Bacterial infection is one of the main factors affecting wound healing. Wound exudate containing large amount of inflammatory cytokines, proteases and free radicals will delay wound healing. Research and development of new compound biological antimicrobial dressings have important implications for treating surgery wound infection, which is the inevitable trend of the development of wound dressings.  OBJECTIVE: To observe the antibacterial activity, moisture absorption capacity and cytotoxicity of calcium alginate dressing containing nanocrystalline silver. METHODS: Silver nanoparticles were added into calcium alginate to prepare new compound biological antimicrobial dressings. The antibacterial activity, moisture absorption capacity and cytotoxicity of calcium alginate dressing containing nanocrystalline silver were detected using the plate count method, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, electron microscope experiment, compared with silver ion calcium alginate dressing and calcium alginate dressing, in the hope of showing the strong antimicrobial activity and low cytotoxicity of new compound biological antimicrobial dressings. RESULTS AND CONCLUSION: The new compound biological nano-silver antimicrobial dressing, compared with the silver ion calcium alginate dressing and calcium alginate dressing, had stronger bacteriostasis to Staphylococcus aureus and Pseudomonas aeruginosa (P < 0.01), and exhibited lower cytotoxicity (P < 0.01). There occurred no significant differences in moisture absorption capacity among the three groups (P > 0.05). It is proved that the new compound biological nano-silver antimicrobial dressing has stronger antibacterial activity and lower cytotoxicity. Figures and Tables | References | Related Articles | Metrics

Select

Cytocompatibility between novel nerve conduit composite materials and bone marrow mesenchymal stem cells Hu Hui, Zhang Wei-cai, Huang Ji-feng, Yan Qiong-jiao, Yin Yi-xia, Li Shi-pu 2013, 17 (16):  2913-2920.  doi: 10.3969/j.issn.2095-4344.2013.16.009 Abstract ( 412 )   PDF (467KB) ( 661 )   Save BACKGROUND: Ideal nerve repair materials are characterized as good biocompatibility, biodegradability, plasticity, and certain mechanical strength. OBJECTIVE: To evaluate the cytocompatibility of rat bone marrow mesenchymal stem cells with (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composites. METHODS: Passage 3 bone marrow mesenchymal stem cells which were isolated from Sprague-Dawley rats were co-cultured with (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials or their extracts, serving as experimental control. Another bone marrow mesenchymal stem cells cultured in the culture medium containing 10% fetal bovine serum served as controls. Cell growth, survival and apoptosis on the composite materials were observed. RESULTS AND CONCLUSION: Results from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that absorbance values in the experimental group were higher than those in the control group after co-culture for days 5 and 7 (P < 0.05). Annexin V-FITC/PI double staining results showed that the apoptosis rate of the experimental group was significantly lower than that of the control group (P < 0.05). Under a scanning electron microscope, bone marrow mesenchymal stem cells grew well on the surface of (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials. Many protrusions ran out from the cells, and they were woven into a mesh, which was typical performance of neuron-like cells. These findings indicate that (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials have good cytocompatibility with bone marrow mesenchymal stem cells, which can be regarded as an excellent carrier for artificial nerve construction. Figures and Tables | References | Related Articles | Metrics

Select

Molecular biocompatibility of a magnesium-zinc alloy implanted in animals Yuan Qing-ling, Yan Jun, Zheng Qi, Zhang Shao-xiang, Zhang Xiao-nong 2013, 17 (16):  2921-2926.  doi: 10.3969/j.issn.2095-4344.2013.16.010 Abstract ( 367 )   PDF (347KB) ( 627 )   Save BACKGROUND: Previous studies have shown that long-term implantation of magnesium-zinc alloy into animals has a good bio-security. OBJECTIVE: To observe the molecular biocompatibility of biodegradable magnesium-zinc alloy samples implanted into the rat cecum. METHODS: Seventy-two Sprague Dawley rats were randomly divided into magnesium-zinc alloy group, medical titanium group and sham-operated group, and there were 24 rats in each group. Then magnesium-zinc alloy samples were embedded in the cecum incision in the magnesium-zinc alloy group. The medical titanium was embedded in the medical titanium group, and just suture was done in the sham-operated group. Prior to and after surgery, alanine transaminase, creatinine, magnesium ion concentrations, serum platelet-derived growth factor concentration and the expression of transforming growth factor-β1 in the cecal tissue were detected by immunohistochemistry. RESULTS AND CONCLUSION: No significant difference was determined in serum aspartate aminotransferase, creatinine, and magnesium ion concentrations, serum platelet-derived growth factor concentration and the expression of transforming growth factor-β1 among the magnesium-zinc alloy group, medical titanium group and sham-operated group at the same time point following surgery (P > 0.05). However, in the magnesium-zinc alloy group, the level of serum platelet-derived growth factor was significantly increased at different time after surgery (P < 0.05). The magnesium-zinc alloy had no harmful effect on wound healing of the rat cecum, and posed a good biocompatibility with the rat cecum. Figures and Tables | References | Related Articles | Metrics

Select

Cytocompatibility of hyaluronic acid hydrogels with concentration variations Li Kai, Lü Shao-yi, Zhou Jian-ye, Shang Qing-qing, Shao Zi-qiang, Hu Sheng-shou 2013, 17 (16):  2927-2934.  doi: 10.3969/j.issn.2095-4344.2013.16.011 Abstract ( 803 )   PDF (513KB) ( 1062 )   Save BACKGROUND: Hydrogel, which is a kind of semi-solid materials, can be used for three-dimensional culture for mesenchymal stem cells. So it would improve efficiency of cultured cells by eliminating “contact inhibition”, which is common in two-dimensional culture. Hyaluronic acid hydrogel as a three-dimensional culture matrix has been reported in the field of stem cells and tissue engineering research. OBJECTIVE: To investigate the morphology and proliferation of rat bone marrow mesenchymal stem cells cultured in the three-dimensional hyaluronic acid hydrogels with different properties. METHODS: Three concentrations of hyaluronic acid hydrogels, 4, 8, and 12 g/L, were prepared and evaluated with their properties including gelation time, swelling ratio, degradation rate and viscoelasticity. Rat bone marrow mesenchymal stem cells were encapsulated and cultured in those hyaluronic acid hydrogels. Another rat bone marrow mesenchymal stem cells cultured in the two-dimensional medium served as control. Morphology of the encapsulated bone marrow mesenchymal stem cells was observed by laser scanning confocal microscope. Growth curves of bone marrow mesenchymal stem cells cultured in the hyaluronic acid hydrogels with different concentrations were depicted using cell counting method. RESULTS AND CONCLUSION: The higher concentration of hyaluronic acid hydrogels leaded to the shorter gelation time, lower swelling ratio, slower degradation rate and higher strength (P < 0.05). Bone marrow mesenchymal stem cells cultured in the three-dimensional hyaluronic acid hydrogels showed a spherical shape and higher proliferation compared with those in normal culture. Meanwhile, rat bone marrow mesenchymal stem cells cultured in 8 g/L hyaluronic acid hydrogels showed higher proliferation than those in 4 and 12 g/L hyaluronic acid hydrogels (P < 0.05). These findings indicate that concentration variations of hyaluronic acid hydrogels lead to changes in their mechanical properties. Hyaluronic acid hydrogel with a concentration of 8 g/L is more appropriate for three-dimensional culture of rat bone marrow mesenchymal stem cells. Figures and Tables | References | Related Articles | Metrics

Select

Controlled drug release capability of paclitaxel-loaded poly(butylcyanoacrylate)-pluronic P123/F68 polymeric micells Zhang Yang-de, Xu Hong-juan, Liang Jian, Wang Ji-wei, Pan Yi-feng, Deng Xin 2013, 17 (16):  2935-2942.  doi: 10.3969/j.issn.2095-4344.2013.16.012 Abstract ( 534 )   PDF (466KB) ( 818 )   Save BACKGROUND: Paclitaxel-loaded poly(butylcyanoacrylate)-pluronic P123 polymeric micells can effectively prolong drug circulation time, and change the paclitaxel targets. However, the stability in solution and drug loading capacity of this kind of paclitaxel polymer micelles need to be improved. OBJECTIVE: To investigate the pharmaceutical characteristics and antitumor ability of paclitaxel-loaded poly(butylcyanoacrylate)-pluronic P123/F68 polymeric micells. METHODS: We prepared the paclitaxel-loaded poly(butylcyanoacrylate)-pluronic P123/F68 polymeric micells using the method of film dispersion and studied their characteristics, such as morphology, size, zeta potential, encapsulation efficiency, drug loading, the critical micelle concentration, drug release, micelle stability and cytotoxity in vitro. RESULTS AND CONCLUSION: The morphology of the prepared micells was spherical with the mean size of 100 nm and the zeta potential of -10 mV. The mean encapsulation efficiency and drug loading were (93.3±2.15)% and (1.82±0.04)%, respectively. The critical micelle concentration was 0.067 g/L. The in vitro release and stability experiments showed that the micells exhibited controlled release ability and good stability. The paclitaxel-loaded poly(butylcyanoacrylate)-pluronic P123/F68 polymeric micells were apparently more potent in killing MCF-7 cells than the free drug. Therefore, paclitaxel-loaded poly(butylcyanoacrylate)-pluronic P123/F68 polymeric micells with good controlled release ability and stability may serve as nanoscopic and long-circulating carriers for poorly water-soluble anticancer drugs. Figures and Tables | References | Related Articles | Metrics

Select

Silk-poly(lactic-co-glycolic acid) copolymer composite scaffold: Mechanical properties and cytocompatibility Zhang Wen-yuan, Yang Ya-dong, Li Ying, Zhang Ke-ji, Fang Guo-jian, Tang Liang, Li Yue-zhong, 2013, 17 (16):  2943-2949.  doi: 10.3969/j.issn.2095-4344.2013.16.013 Abstract ( 382 )   PDF (492KB) ( 451 )   Save BACKGROUND: Compared with the usual synthetic fibers, silk has better mechanical properties with a certain degree of ductility, which is a good scaffold material for construction of tissue engineered ligament/tendon. The degradation speed of silk fibroin fibers, however, is too low to correspond to tissue regeneration rate. OBJECTIVE: To evaluate the mechanical property of a rope-shaped scaffold braided by silk-poly(lactic-co-glycolic acid) and its cytocompatibility with rabbit bone marrow mesenchymal stem cells. METHODS: Firstly, the hybrid rope-shaped silk-poly(lactic-co-glycolic acid) scaffold was prepared by twisting weave using a mixture of silkworm silk fibroin fiber and PLGA filament, and then modified by fibronectin. Secondly, rabbit bone marrow mesenchymal stem cells were isolated and proliferated in vitro. Then, they were seeded on the surface of the scaffold. The situation of cell growth, matrix formation, as well as the combination of cells and scaffold was observed under an inverted phase contrast microscope and scanning electron microscopy. RESULTS AND CONCLUSION: The appearance of the scaffold braided by silk-poly(lactic-co-glycolic acid) was milky white, spiral rope-like, and homogeneous with a strong toughness. The diameter of the scaffold was 2.3 mm. The maximum load, tensile strength, breakpoints elongation rates, and elastic modulus of the scaffold were (315.06±30.77) N, (75.83±7.46) MPa, (61.39±7.26)%, (213.58±23.45) MPa, respectively. Scanning electron microscopy observation revealed that rabbit bone marrow mesenchymal stem cells grew and adhered well on the scaffold surface. Most of the bone marrow mesenchymal stem cells showed spindle-shaped with good stretch, and they grew in three dimensions. Moreover, the bone marrow mesenchymal stem cells secreted rich cell matrix on the surface of the scaffold braided by silk-poly(lactic-co-glycolic acid). In short, the scaffold braided by silk-poly(lactic-co-glycolic acid) has good biomechanical properties and cytocompatibility.  Figures and Tables | References | Related Articles | Metrics

Select

Reconstructing the perfused-decellularized full-thickness small intestine extracellular matrix Hou Nan, Zhu Li, Wen Ke 2013, 17 (16):  2950-2955.  doi: 10.3969/j.issn.2095-4344.2013.16.014 Abstract ( 408 )   PDF (344KB) ( 486 )   Save BACKGROUND: Immersing method is difficult to construct the ideal acellular matrix for the complex organ. OBJECTIVE: To investigate the effect of preparing the acellular full-thickness small intestine scaffold by perfusion method. METHODS: All the small intestines were excised in a sterile fashion in adult male New Zealand rabbits and the acellular small intestine scaffolds were obtained by perfusing ionic detergents through the superior mesenteric arteries. Relative growth rates were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide method after bone marrow mesenchymal stem cells from 1-month-old NewZealand rabbits were co-cultured with decellularized full-thickness small intestine extracellular matrix (experimental group) or Dulbecco’s modified Eagle’s medium containing 20% fetal bovine serum (control group). RESULTS AND CONCLUSION: The macroscopic view showed that the perfused-decellularized small intestine artery became translucent after 30 minutes of perfusion, and the small intestine segment became translucent after 2 hours of perfusion. The vessels were seen distinctly. Histological view and scanning electron microscope results indicated that the perfused-decellularized full-thickness small intestine showed perfect acellular effect. Many pores and collagen fibers were retained and the porosity was (86.72±2.98)%. 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide assay showed that the relative growth rate of the experimental group overtopped 1. The absorbance values in the experimental group were significantly higher than those in the control group at 2, 4 and 7 days of coculture (P < 0.05). These findings suggest that the perfusion method is a better way to construct acellular full-thickness small intestine scaffold because it can achieve better acellular effect and retain scaffold vitality at the greatest extent in the acellular small intestine scaffold that can promote the growth of bone marrow mesenchymal stem cells and not add toxicity to the cells. Figures and Tables | References | Related Articles | Metrics

Select

Stress relaxation properties of fetal umbilical cord vein grafts Xu Dong-hui, Li Dong-yuan, Li Xin-ying, Ding Yue-hong 2013, 17 (16):  2956-2960.  doi: 10.3969/j.issn.2095-4344.2013.16.015 Abstract ( 546 )   PDF (290KB) ( 522 )   Save BACKGROUND: Human umbilical cord vein can serve as a good substitute for arterial grafts. Compliance of graft materials is a key problem during the transplantation expect for the matching between the umbilical   cord vein and host’s artery. OBJECTIVE: To detect the stress relaxation properties of fetal umbilical vein samples proximal and distal to the placenta. METHODS: Fetal umbilical vein samples at the gestational age of 38-40 were taken from spontaneous delivery parturient women aged 22-28 years. Ten proximal placenta segments and 10 distal placenta segments were taken and placed on the electronic universal testing machine to do the stress relaxation test at (36.5±0.5) ℃. The strain was applied to the samples at a strain increases speed of 0.1%/s, and the setting time was 7 200 seconds. Totally 100 data were collected; and then, the normalized analysis method was employed to calculate the normalized stress relaxation equation. RESULTS AND CONCLUSION: The stress at 7 200 seconds was decreased by 0.275 MPa in the proximal placenta segment group and 0.203 MPa in the distal placenta segment group. There was a significant difference between the two segment samples (P < 0.05). The stress relaxation curves of the two groups were changed with logarithmic relationship, and the proximal placenta segment and the distal placenta segment of fetal umbilical cord vein had different stress relaxation properties. Figures and Tables | References | Related Articles | Metrics

Select

Collagen scaffold facilitates the three-dimensional culture of myocardial cells from adult rats in vitro Wang Hui-ling, Li Qiong, Guo Zhi-kun, Sun Yong-kun, Kuboki Y 2013, 17 (16):  2961-2967.  doi: 10.3969/j.issn.2095-4344.2013.16.016 Abstract ( 534 )   PDF (391KB) ( 506 )   Save BACKGROUND: Three-dimensional contractible myocardial cell block of neonatal rats in the Atelocollagen scaffold has been cultured in the previous study. OBJECTIVE: To perform three-dimensional culture of myocardial cells from adult rats on the Atelocollagen scaffold. METHODS: After being cultured and purified, myocardial cells from adult Sprague-Dawley rats were implanted onto the Atelocollagen scaffold for three-dimensional cultures. The growth of myocardial cells on the Atelocollagen scaffold was dynamically observed by inverted microscopy. Myocardial cell blocks were separately tested by hematoxylin-eosin staining, immunohistochemistry and immunofluorecence of troponin after frozen section. RESULTS AND CONCLUSION: After being implanted, myocardial cells from adult Sprague-Dawley rats began to grow on the Atelocollagen scaffold in 12 hours, showed mutual integration with the scaffold and the integration became more closely 24 hours later. However, the myocardial cells did not have autorhythmiciting beats at all, and among the cells there were some cell debris. After replaced with the same fresh medium 48 hours later, the cell debris was washed away and most of the rest cells grew on the edge of the Atelocollagen scaffold. After 48 hours, the cells formed a complex with the scaffold tested by hematoxylin-eosin staining, and the cells which grew well and were coalesced with the scaffold meshes were mainly myocardial cells, identified by immunohistochemistry and immunofluorecence. Figures and Tables | References | Related Articles | Metrics

Select

Acellular tendon of mini-pig inbred-lines is used for Achilles tendon repair in a rabbit model Jin Xian-hui, Zhang Qing-sheng, Yang Jian-bo, Liu Gui-hua, Wei Wei, Gao Chun-guang, Lin Yue-qiu 2013, 17 (16):  2968-2972.  doi: 10.3969/j.issn.2095-4344.2013.16.017 Abstract ( 390 )   PDF (551KB) ( 493 )   Save BACKGROUND: Allogeneic tendon graft is an ideal method to repair tendon defects. But post-transplantation rejections limit the use of allogeneic tendons. OBJECTIVE: To investigate the effect of acellular tendon of Banna mini-pig inbred-lines on repair of rabbit Achiles tendon, and then to evaluate the feasibility and validity of porcine-derived xenogeneic graft. METHODS: Forty Japanese white rabbits were enrolled to prepare Achilles tendon defect models of the both hind legs, and then were randomly divided into two groups: acellular porcine tendon group treated with acellular tendon from Banna mini-pig inbred-lines and autologous tendon group treated with rabbit autologous tendon. End-to-end anastomosis was performed with a 3-0 tendon thread using improved HEMI-KESSLER method. RESULTS AND CONCLUSION: Within 2 weeks post transplantation, there were no significant differences in white blood cell count and C-reactive protein level between the two groups (P > 0.05). Local reactions in the two groups after transplantation were few, the wound healed at stage Ⅰ, and flexor ankle function returned to normal. Histological results exhibited no lymphocytic infiltration, but interconnected collagen fibers at tendon suture site. These findings indicate that acellular tendons from Banna mini-pig inbred-lines can successfully reconstruct defected rabbit Achilles tendon with good histocompatibility and little rejection. Figures and Tables | References | Related Articles | Metrics

Select

Layer-by-layer self-assembly multifunctional heparin surface modification Wei Wei, Li Guo-rong 2013, 17 (16):  2973-2980.  doi: 10.3969/j.issn.2095-4344.2013.16.018 Abstract ( 456 )   PDF (540KB) ( 753 )   Save BACKGROUND: Surface coating can improve material surface properties and hemocompatibility without changing the original material, and thus reduce complications resulting from implantable devices. OBJECTIVE: To observe the hemocompatibility and anticoagulation properties of layer-by-layer self-assembly multifunctional heparin coating. METHODS: Layer-by-layer self-assembly was used to form the self-assembled multilayer coating with two function layers on the surface and the bottom. Surface coating was heparin/Fe 3+ multi-layer self-assembly coating and the bottom coating was chitosan, both of which were fastened to the surface of titanium using a covalent bond. There were five layers of heparin. The heparin/chitosan layer-by-layer self-assembly coating with the same number of layers was considered as the multi-layer control group, and the single layer coating of heparin and chitosan blending solution was considered as the single-layer control group; the bare titanium pieces were regarded as the blank control group. RESULTS AND CONCLUSION: Examination of hemocompatibility showed that the hemolysis rate of the self-assembled multilayer coating was lower than that of the multi-layer control group; toluidine blue staining showed that the total amount of heparin and the heparin dissolved and released amount at 4, 24 and 48 hours of the self-assembled multilayer coating were significantly higher than those of multi-layer control group and single- layer control group; dynamic clotting experimental results showed the self-assembled multilayer coating had the best anticoagulation properties. The results indicate that layer-by-layer self-assembly multifunctional heparin coating has the excellent hemocompatibility and coagulation properties. Figures and Tables | References | Related Articles | Metrics

Select

Inflammatory factor release from leukocyte- and platelet-rich plasma gel Wen Tian-yang, Wang Ai-hong, Xu Zhang-rong 2013, 17 (16):  2981-2988.  doi: 10.3969/j.issn.2095-4344.2013.16.019 Abstract ( 595 )   PDF (421KB) ( 507 )   Save BACKGROUND: Current studies about leukocyte- and platelet-rich plasma gel mainly focus on growth factor release and the mechanism underlying promoting tissue growth and wound healing. OBJECTIVE: To investigate the mechanism of leukocyte- and platelet-rich plasma gel by measuring the levels of interleukin-1, interleukin-4, interleukin-6, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor released from leukocyte- and platelet-rich plasma gel and observing the ultrastructure of leukocyte- and platelet-rich plasma gel. METHODS: Blood samples were collected from 12 healthy volunteers. Leukocyte- and platelet-rich plasma was produced by two-step centrifugation, and leukocyte- and platelet-rich plasma gel was obtained by combining the leukocyte- and platelet-rich plasma with thrombin and calcium gluconate (10:1). The leukocyte- and platelet-rich plasma gel was preserved in Dulbecco’s modified Eagle’s medium, and the Dulbecco’s modified Eagle’s medium including exudates of leukocyte- and platelet-rich plasma gel was collected at days 1, 3, 7, 14, 21. RESULTS AND CONCLUSION: Enzyme linked immunoabsorbent assay showed that the leukocyte formula in the leukocyte- and platelet-rich plasma gel was different from that in the whole blood, and lymphocytes shared the largest proportion. Interleukin-1 level peaked at 0-1 day of culture (P < 0.05), and then gradually decreased. Interleukin-4 level showed no changes at different periods. Interleukin-6 maintained a higher release at 0-1, 2-3, and 4-7 days, but dramatically reduced at 8-14 and 15-21 days of culture (P < 0.05). The release amount of platelet-derived growth factor-BB, transforming growth factor-β1 reached the peak at 1 day of culture (P < 0.05), and then exhibited a raped decline. The release amount of vascular endothelial growth factor gradually increased at 7 days of culture, and then gradually declined at 8-21 days of culture. Under the scanning electron microscope, vast majority of leukocytes in the leukocyte- and platelet-rich plasma gel were confirmed as lymphocytes. Results showed that besides the expression of growth factors such as platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor, the concentrations and components change of lymphocytes and expression of inflammatory factors such as interleukin-1, interleukin-4, and interleukin-6 might be one of the mechanisms by which leukocyte- and platelet-rich plasma gel can promote wound healing. Figures and Tables | References | Related Articles | Metrics

Select

Preparation and modification of nano-hydroxyapatite Pang Gui-hua, Cheng Zhi-qiang, Li Jun-feng 2013, 17 (16):  2989-2993.  doi: 10.3969/j.issn.2095-4344.2013.16.020 Abstract ( 1305 )   PDF (362KB) ( 1206 )   Save BACKGROUND: The prepared hydroxyapatite is likely agglomerated due to its specific surface area. OBJECTIVE: To investigate the agglomeration problem of hydroxyapatite and to improve the dispersity of hydroxyapatite. METHODS: The nano-hydroxyapatite was prepared using solution coprecipitation method from calcium nitrate and ammonium di-hydrogen phosphate and further modified by combination of polyethylene glycol (2%, 4%, 5%, 6%, 8%), polyvinyl alcohol (2%, 4%, 5%, 6%, 8%), and stearic acid (2%, 4%, 5%, 6%, 8%). RESULTS AND CONCLUSION: Nano-hydroxyapatite was successfully obtained through X-ray diffraction analysis. The type of surface modifying agent and active agent concentration could affect the particle size of the hydroxyapatite when cladded by them. After considering the influential factors in processes of synthesis and modification, the following conclusions can be determined: Polyethylene glycol is the best modifying agent for hydroxyapatite in all three modifying agents which involve polyethylene glycol, polyvinyl alcohol and stearic acid. Furthermore, the dispersity of the hydroxyapatite is excellent after modification by 5% polyethylene glycol. Figures and Tables | References | Related Articles | Metrics

Select

Preparation and characterization of hepatocyte-specific macroporous microcarrier Guo Wei, Gong Du-hui, Hu Zhi-wei, Cheng Yuan, Gao Yi, Pan Ming-xin 2013, 17 (16):  2994-3001.  doi: 10.3969/j.issn.2095-4344.2013.16.021 Abstract ( 595 )   PDF (728KB) ( 652 )   Save BACKGROUND: Hepatocyte culture is the key to the hepatocyte transplantation and bioartificial liver study. It has turned into the first problem how to get adequate number of better activity and well-functioning hepatocytes. OBJECTIVE: To prepare a high-performance silk fibroin/galactosylated chitosan macroporous microcarrier with hepatocyte-specific characteristics, for hepatocyte adhesion culture in the three-dimensional environment in vitro. METHODS: Silk fibroin, chitosan, and lactose acid served as raw materials to prepare a hepatocyte-specific silk fibroin/galactosylated chitosan macroporous microcarrier using comprehensive application of emulsified-chemical crosslinking, polarity solution and freeze-drying. Optical microscope, scanning electron microscope, proton nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and cell biocompatibility test were used to characterize its morphology, physical and chemical properties. RESULTS AND CONCLUSION: The microcarrier was successfully prepared in the project. Furthermore, the pores were occupied inside and outside of the microcarrier and interconnected, which presented hepatocyte-specific characteristics. Under the scanning electron microscope, the macroporous microcarrier exhibited a trumpet-shaped porous structure with the pore size of 40-80 µm and even pore distribution, providing an appropriate environment for high-density culture of hepatocytes. The galactosyl was successfully compounded into the scaffold material which was confirmed by the proton nuclear magnetic resonance spectroscopy and Fourier transform infrared spectroscopy. Cell biocompatibility test showed a hepatocyte-specific affinity to the silk fibroin/galactosylated chitosan macroporous microcarrier, and the cells grew and aggregated around the microcarrier exhibiting a good activity. These findings indicate that combination of emulsified-chemical crosslinking, polarity solution and freeze-drying can prepare a silk fibroin/galactosylated chitosan macroporous microcarrier with hepatocyte-specific characteristics. Figures and Tables | References | Related Articles | Metrics

Select

Antimicrobial composite resin: Antibacterial ability Che Xiao-qiang, Zhang Song-ying, Chen Yun-ping, Liu Bin 2013, 17 (16):  3002-3009.  doi: 10.3969/j.issn.2095-4344.2013.16.022 Abstract ( 311 )   PDF (482KB) ( 749 )   Save BACKGROUND: Antibacterial agents with good antibacterial effects, such as nano silver, amine salt, and calcium phosphate, have a growth inhibition on common pathogenic bacteria in the mouth. Recently, it is a hot issue to make antimicrobial composite resins by adding the antibacterial agents to the composite resin. OBJECTIVE: To summarize recent studies on antimicrobial composite resin. METHODS: Databases of CNKI, PubMed, Wanfang, VIP, Web of Science, and Nature from January 1995 to May 2012 were searched by computer with key words of “composite resin, antibacterial agent, silver ion, amine salt, calcium phosphate” both in Chinese and English. Literatures concerning antibacterial agents and antimicrobial composite resin were included, and repetitive research was excluded. RESULTS AND CONCLUSION: Antibacterial agents are classified into natural, organic, inorganic, polymer agents. Currently, antimicrobial composite resins carrying nano sliver, amine salt, and calcium phosphate are often reported. The vivo and vitro experiments show that the composite resin with antibacterial agents has good antibacterial properties, which can inhibit the growth of bacteria, reduce the occurrence of recurrent caries, and prolong the in-service life of the prosthesis, and its physical and chemical properties have no apparent changes. In the latest study, the composite resin has better antibacterial properties and mechanical functions by addition with all kinds of antibacterial agents, which will be the main direction of studying the novel antimicrobial composite resin in the future. Figures and Tables | References | Related Articles | Metrics

Select

Synthetic nerve conduit versus autogenous nerve transplantation for repair of peripheral nerve defects Luo Peng, Peng Qiu-liang, Xiang Jian-ping, Qi Jian 2013, 17 (16):  3010-3017.  doi: 10.3969/j.issn.2095-4344.2013.16.023 Abstract ( 345 )   PDF (503KB) ( 929 )   Save BACKGROUND: As the biodegradable materials produced nerve conduit can be degraded in vivo and can avoid the nerve entrapment, it has attracted more and more attentions. OBJECTIVE: To compare the effect of autogenous nerve transplantation and three kinds of synthetic biodegradable materials produced nerve conduit for the repair of peripheral nerve defects. METHODS: The effect of commonly used collagen nerve conduit, DL-lactic acid-ε-caprolactone nerve conduit, polyglycolic acid nerve conduit and autogenous nerve transplantation in the repair of peripheral nerve defects was evaluated with electrophysiological detection and morphological observation. RESULTS AND CONCLUSION: Theoretically, the nerve conduit has some advantages when compared with autogenous nerve transplantation, but there was significant difference in neural functional recovery between different synthetic materials produced nerve conduits. The repair effect of DL-lactic acid-ε-caprolactone nerve conduit was similar to that of autogenous nerve transplantation, and was considered as the ideal nerve conduit material; due to the disadvantages of polyglycolic acid nerve conduit which can inhibit its degradation, polyglycolic acid nerve conduit showed least effect in repairing peripheral nerve defects among three kinds of nerve conduits; collagen nerve conduit could improve the mechanical properties with the help of crosslinker, so the effect in repairing peripheral nerve defects was lower than DL-lactic acid-ε-caprolactone nerve conduit and higher than polyglycolic acid nerve conduit. These three kinds of nerve conduits have their potential shortcomings in nerve function regeneration, so that can not completely replace autogenous nerve transplantation. There still lacks of large sample long-term randomized controlled experiments to identify the performance price ratio between three kinds of nerve conduits. Further experimental observations are needed. Figures and Tables | References | Related Articles | Metrics

Select

Stress and strain of anterior region cantilever fixed partial denture and bone tissue: Three-dimensional finite element analysis Wang Fei-fei, Chen Zu-xian 2013, 17 (16):  3018-3025.  doi: 10.3969/j.issn.2095-4344.2013.16.024 Abstract ( 578 )   PDF (490KB) ( 501 )   Save BACKGROUND: There are still some controversies for the number, position as well as the force of the implants and the bridge during the clinical repairing of dentition defects with cantilever fixed partial denture.   OBJECTIVE: To analyze the force conditions of anterior region cantilever fixed partial denture with three-dimensional finite element. METHODS: The computer was used to simulate the three-dimensional finite element method to analyze the implant-supported fixed dentures, as well as the stress and strain status of implants and surrounding bone tissues with different numbers and different positions of implants located on the cantilever fixed partial denture, in order to provide guiding significance for the clinical application. RESULTS AND CONCLUSION: When implanted with the same load force and load angle, the maximum equivalent stress of the implants and the surrounding bone was gradually increased with the reducing of the implant number and the increasing of the bridge length, and the minimum safety coefficient was decreased gradually. When the load angle was 22.5°, implants and the surrounding bone showed the lowest maximum stress, and the minimum safety coefficient was highest. With the increasing of the load angle, the maximum stress of the implants and the surrounding bone was increased gradually, and the minimum safety coefficient was decreased. The distribution of the stress was uneven before and after repaired with cantilever fixed partial denture, and stress distribution in supporting bone of abutment mainly concentrated in neck. For the three units of edentulous area, the success rate of repairing was positively correlated with the number of abutment. Reasonable choice of cantilever fixed partial denture and rigidly fixed bridge is the key for the successful of the treatment. Figures and Tables | References | Related Articles | Metrics

Select

Application of clearance flush vacuum-sealing drainage based on wound dressing in chronic wounds Xu Hai-dong, Zhao Jian-ning, Lu Jun-hao, Chen Yong 2013, 17 (16):  3026-3032.  doi: 10.3969/j.issn.2095-4344.2013.16.025 Abstract ( 685 )   PDF (260KB) ( 998 )   Save BACKGROUND: Preliminary studies have found that local wound gory, tissue secretions or bacteria growth can result in tube jam after treatment with vacuum-sealing drainage based on biomaterial wound surface dressing, and we cannot get satisfied results in the repair of soft tissues below the blocked tube area. OBJECTIVE: To study the curative effect of clearance flush vacuum-sealing drainage based on biomaterial dressing in the treatment of chronic wounds. METHODS: Thirty-six patients with chronic wounds were divided into three groups which were cured by clearance flush vacuum-sealing drainage based on biomaterial dressing, vacuum-sealing drainage based on synthetic dressing and conventional dressings by their willingness. RESULTS AND CONCLUSION: The bacteria detection rate of clearance flush vacuum-sealing drainage based on biomaterial wound surface dressing group was inferior to the synthetic dressing group and conventional dressing group (P < 0.05). And the healing time was shorter in the former one group (P < 0.05). The score on the visual analogue pain in the clearance flush vacuum-sealing drainage based on biomaterial wound surface dressing group was better than that in the conventional dressing group (P < 0.05), but did not significantly differ from that in the synthetic dressing group (P > 0.05). These findings indicate that clearance flush vacuum-sealing drainage based on biomaterial wound surface dressing can promote chronic wound healing, reduce the wound infection rate, relieve the pain of patients when treatment, and have no adverse.   Figures and Tables | References | Related Articles | Metrics

Select

Long-term comparison of three bone graft materials in lumbar interbody fusion for lumbar spondylolisthesis Yuan Zhen-chao, Chen Yuan-ming, Chen Feng, Liu Wan-xiang 2013, 17 (16):  3033-3040.  doi: 10.3969/j.issn.2095-4344.2013.16.026 Abstract ( 413 )   PDF (325KB) ( 544 )   Save BACKGROUND: Interbody fusion is an important method for lumbar spondylolisthesis. The effects of different bone materials require further studies. OBJECTIVE：To compare the effects of autologous bone, allogeneic bone, bone morphogenetic protein composite bone in patients with lumbar spondylolisthesis. METHODS: Totally 119 cases of lumbar spondylolisthesis were selected and randomly divided into three groups: autologous bone group (40 cases), allogeneic bone group (41 cases), bone morphogenetic protein composite bone group (38 cases). Intervertebral height and fusion rate were compared after fusion as well as long-term clinical efficacy. RESULTS AND CONCLUSION: Compared with the allogeneic bone group, autologous bone group and bone morphogenetic protein composite bone group were better in the maintenance of intervertebral height. Postoperative fusion rates were ranked as follows: composite bone group > autologous bone group > allogeneic bone group (P < 0.05). At 9 and 12 months postoperatively, the fusion rates in the composite bone group were better than those in the autologous bone group (P < 0.05). Nakai scores in the autologous bone and composite bone groups were superior to that in the allogeneic bone group (P < 0.05). There was a significant difference in Nakai scores among the three groups (P < 0.05). These findings indicate that bone morphogenetic protein composite bone is similar to autologous bone but superior to allogeneic bone in the maintenance of intervertebral height, fusion rate and clinical efficacy. Figures and Tables | References | Related Articles | Metrics