Subcutaneous osteogenesis by bone marrow stromal cells combined with sintered bone

doi:10.3969/j.issn.2095-4344.2013.16.005

Abstract

Abstract:

BACKGROUND: It has proved that the bone marrow stromal cells and sintered bone support material combination can form tissue-engineered bone, but relevant studies on its biocompatibility in animals and subcutaneous osteogenesis ability are less reported in China.
OBJECTIVE: To observe the osteogenesis capacity of marrow stromal stem cells combined with sintered bone implanted into Balb/c nude mouse back subcutaneously and to explore the feasibility of sintered bone as a scaffold for tissue-engineered bone.
METHODS: Sintered bone scaffold materials were prepared using defatted and deproteinized processing and high-temperature calcinations. Then the sintered bone was combined with passage 3 sheep bone marrow stromal stem cells cultured using density gradient centrifugation method to be implanted subcutaneously into the back of BALB/c nude mice. Simple sintered bone that was subcutaneously implanted into the contralateral back of BALB/c nude mice served as control group.
RESULTS AND CONCLUSION: After calcinations, sintered cancellous bone block colored chalks and the surface displayed a honeycomb porous structure, maintaining the porous structure of the natural cancellous bone. Bone trabecular structure was of integrity, and pores were mutually interconnected. After bone marrow stromal stem cells were inoculated to the calcined bone, there were a lot of cells adherent to the scaffold within 24 hours, and a large amount of extracellular matrix at 7 days. The boundary between cells and extracellular matrix was unclear, and the cells could grow and proliferate well on the scaffold, suggesting that the cell viability was not influenced by the scaffold. Four weeks after implantation, a few of fragments were visible at the edge of sintered bone in the two groups. Bone cells could be seen around the pores of sintered bone in the combination group, while fibrous connective tissue enveloped the sintered bone in the control group. Eight weeks later, the sintered bone partially degraded into osteoid sheets surrounded by fibroblasts that arranged closely and was diverse in shape. In the combination group, osteoblasts arranged in line on the surface of sintered bone and infiltrated lymphocytes were scattered among the pores. In the control group, a great amount of connective tissues grew into the pores, but there was no osteogenesis. These findings indicate that the sintered cancellous bone block pose better biocompatibility and biological safety, which can be a better scaffold for bone marrow stromal stem cells. The composite of bone marrow stromal stem cells and sintered bone has a good ability to induce new bone formation and the sintered bone can be used as scaffold materials for tissue engineering repair of bone defects.

Key words: biomaterials, tissue-engineered bone materials, sintered bone, bone marrow stromal stem cells, bone defects, subcutaneous osteogenesis, implantation, Balb/c nude mice, bone marrow-derived stem cells, bone tissue engineering, National Natural Science Foundation of China

CLC Number:

R318

Yang Chuan-bo, He Hui-yu, Cui Jie, Ma Wen-yuan, Yang Nan. Subcutaneous osteogenesis by bone marrow stromal cells combined with sintered bone[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2883-2890.

Figures/Tables 6

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